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Emergence of NDM-1- and IMP-14a-producing Enterobacteriaceae in Thailand.
J Antimicrob Chemother. 2012 Nov; 67(11):2626-30.JA

Abstract

OBJECTIVES

To detect carbapenemases in clinical isolates of Enterobacteriaceae collected from patients in a university hospital in Thailand between October 2010 and August 2011.

METHODS

A total of 4818 Enterobacteriaceae isolates were screened for the presence of carbapenemases by ertapenem and imipenem disc diffusion tests. All positive screening isolates were subjected to modified Hodge test, phenylboronic acid- and EDTA-carbapenem combined disc tests and two multiplex PCRs of bla(IMP), bla(VIM), bla(SPM), bla(SIM) and bla(GIM), and of bla(KPC), bla(NDM) and bla(OXA-48). Carbapenemase-producing isolates were typed by PFGE and then characterized by antimicrobial susceptibility tests. Conjugation was performed using a broth culture mating method.

RESULTS

Two isolates each of Escherichia coli, Klebsiella pneumoniae and Citrobacter freundii produced NDM-1, whereas two other isolates of K. pneumoniae produced IMP-14a. DNA fingerprints revealed that the metallo-β-lactamase (MBL)-producing isolates were of different strains except for clonal strains of C. freundii. In vitro transfer of carbapenem resistance was successful for the eight MBL-producing isolates. All MBL producers were susceptible to colistin and tigecycline. The six NDM-producing isolates were recovered from the urine of three patients, who had no history of travel outside Thailand. Interestingly, one patient had chronic urinary tract infections caused by a K. pneumoniae strain and two strains of E. coli producing NDM-1.

CONCLUSIONS

Surveillance of carbapenemases, particularly NDM-1, in Enterobacteriaceae is urgently needed to control and prevent the spread of these resistance determinants in our country.

Authors+Show Affiliations

Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22796889

Citation

Rimrang, Benchamas, et al. "Emergence of NDM-1- and IMP-14a-producing Enterobacteriaceae in Thailand." The Journal of Antimicrobial Chemotherapy, vol. 67, no. 11, 2012, pp. 2626-30.
Rimrang B, Chanawong A, Lulitanond A, et al. Emergence of NDM-1- and IMP-14a-producing Enterobacteriaceae in Thailand. J Antimicrob Chemother. 2012;67(11):2626-30.
Rimrang, B., Chanawong, A., Lulitanond, A., Wilailuckana, C., Charoensri, N., Sribenjalux, P., Phumsrikaew, W., Wonglakorn, L., Kerdsin, A., & Chetchotisakd, P. (2012). Emergence of NDM-1- and IMP-14a-producing Enterobacteriaceae in Thailand. The Journal of Antimicrobial Chemotherapy, 67(11), 2626-30. https://doi.org/10.1093/jac/dks267
Rimrang B, et al. Emergence of NDM-1- and IMP-14a-producing Enterobacteriaceae in Thailand. J Antimicrob Chemother. 2012;67(11):2626-30. PubMed PMID: 22796889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Emergence of NDM-1- and IMP-14a-producing Enterobacteriaceae in Thailand. AU - Rimrang,Benchamas, AU - Chanawong,Aroonwadee, AU - Lulitanond,Aroonlug, AU - Wilailuckana,Chotechana, AU - Charoensri,Nicha, AU - Sribenjalux,Pipat, AU - Phumsrikaew,Waewta, AU - Wonglakorn,Lumyai, AU - Kerdsin,Anusak, AU - Chetchotisakd,Ploenchan, Y1 - 2012/07/13/ PY - 2012/7/17/entrez PY - 2012/7/17/pubmed PY - 2013/2/28/medline SP - 2626 EP - 30 JF - The Journal of antimicrobial chemotherapy JO - J. Antimicrob. Chemother. VL - 67 IS - 11 N2 - OBJECTIVES: To detect carbapenemases in clinical isolates of Enterobacteriaceae collected from patients in a university hospital in Thailand between October 2010 and August 2011. METHODS: A total of 4818 Enterobacteriaceae isolates were screened for the presence of carbapenemases by ertapenem and imipenem disc diffusion tests. All positive screening isolates were subjected to modified Hodge test, phenylboronic acid- and EDTA-carbapenem combined disc tests and two multiplex PCRs of bla(IMP), bla(VIM), bla(SPM), bla(SIM) and bla(GIM), and of bla(KPC), bla(NDM) and bla(OXA-48). Carbapenemase-producing isolates were typed by PFGE and then characterized by antimicrobial susceptibility tests. Conjugation was performed using a broth culture mating method. RESULTS: Two isolates each of Escherichia coli, Klebsiella pneumoniae and Citrobacter freundii produced NDM-1, whereas two other isolates of K. pneumoniae produced IMP-14a. DNA fingerprints revealed that the metallo-β-lactamase (MBL)-producing isolates were of different strains except for clonal strains of C. freundii. In vitro transfer of carbapenem resistance was successful for the eight MBL-producing isolates. All MBL producers were susceptible to colistin and tigecycline. The six NDM-producing isolates were recovered from the urine of three patients, who had no history of travel outside Thailand. Interestingly, one patient had chronic urinary tract infections caused by a K. pneumoniae strain and two strains of E. coli producing NDM-1. CONCLUSIONS: Surveillance of carbapenemases, particularly NDM-1, in Enterobacteriaceae is urgently needed to control and prevent the spread of these resistance determinants in our country. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/22796889/Emergence_of_NDM_1__and_IMP_14a_producing_Enterobacteriaceae_in_Thailand_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dks267 DB - PRIME DP - Unbound Medicine ER -