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Effect of inositol hexaphosphate-loaded red blood cells (RBCs) on the rheology of sickle RBCs.
Transfusion. 2013 Mar; 53(3):627-36.T

Abstract

BACKGROUND

The recent in vitro demonstration that inositol hexaphosphate-loaded red blood cells (IHP-RBCs) may reduce the risks of sickling of sickle RBCs (SS RBCs) exposed to hypoxia make these modified RBCs potentially useful in transfused sickle cell anemia (SCA) patients.

STUDY DESIGN AND METHODS

Hemorheologic properties of IHP-RBCs, normal RBCs (AA RBCs), SS RBCs, SS RBCs plus AA RBCs, and SS RBCs plus IHP-RBCs were compared under normoxia and/or after hypoxic challenges.

RESULTS

Although IHP-RBCs have reduced deformability compared with SS RBCs or AA RBCs, IHP-RBCs exhibited lower aggregability than AA RBCs and SS RBCs and, when mixed with SS RBCs, the aggregation level was below the one of SS RBCs alone or SS RBCs plus AA RBCs. Blood viscosity of SS RBC plus IHP-RBC suspension was lower than the viscosity of SS RBCs alone and greater than viscosity of SS RBCs plus AA RBCs. The hypoxic challenge was detrimental for deformability and viscosity of SS RBCs alone or SS plus AA RBC suspension but not for SS plus IHP-RBC suspension.

CONCLUSION

Our results support the fact that IHP-RBCs could be useful in SCA by decreasing RBC aggregation and blunting the adverse effects of hypoxia on RBC deformability and blood viscosity.

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Authors+Show Affiliations

Lamarre Y
UMR Inserm U665, Pointe-à-Pitre, Guadeloupe, France.
Bourgeaux V
No affiliation info available
Pichon A
No affiliation info available
Hardeman MR
No affiliation info available
Campion Y
No affiliation info available
Hardeman-Zijp M
No affiliation info available
Martin C
No affiliation info available
Richalet JP
No affiliation info available
Bernaudin F
No affiliation info available
Driss F
No affiliation info available
Godfrin Y
No affiliation info available
Connes P
No affiliation info available

MeSH

Anemia, Sickle CellBlood ViscosityErythrocyte AggregationErythrocytesErythrocytes, AbnormalHemorheologyHumansOsmotic FragilityPhytic AcidShear StrengthStress, Mechanical

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

22804873

Citation

Lamarre, Yann, et al. "Effect of Inositol Hexaphosphate-loaded Red Blood Cells (RBCs) On the Rheology of Sickle RBCs." Transfusion, vol. 53, no. 3, 2013, pp. 627-36.
Lamarre Y, Bourgeaux V, Pichon A, et al. Effect of inositol hexaphosphate-loaded red blood cells (RBCs) on the rheology of sickle RBCs. Transfusion. 2013;53(3):627-36.
Lamarre, Y., Bourgeaux, V., Pichon, A., Hardeman, M. R., Campion, Y., Hardeman-Zijp, M., Martin, C., Richalet, J. P., Bernaudin, F., Driss, F., Godfrin, Y., & Connes, P. (2013). Effect of inositol hexaphosphate-loaded red blood cells (RBCs) on the rheology of sickle RBCs. Transfusion, 53(3), 627-36. https://doi.org/10.1111/j.1537-2995.2012.03779.x
Lamarre Y, et al. Effect of Inositol Hexaphosphate-loaded Red Blood Cells (RBCs) On the Rheology of Sickle RBCs. Transfusion. 2013;53(3):627-36. PubMed PMID: 22804873.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of inositol hexaphosphate-loaded red blood cells (RBCs) on the rheology of sickle RBCs. AU - Lamarre,Yann, AU - Bourgeaux,Vanessa, AU - Pichon,Aurélien, AU - Hardeman,Max R, AU - Campion,Yannick, AU - Hardeman-Zijp,Marise, AU - Martin,Cyril, AU - Richalet,Jean-Paul, AU - Bernaudin,Françoise, AU - Driss,Françoise, AU - Godfrin,Yann, AU - Connes,Philippe, Y1 - 2012/07/15/ PY - 2012/7/19/entrez PY - 2012/7/19/pubmed PY - 2013/5/1/medline SP - 627 EP - 36 JF - Transfusion JO - Transfusion VL - 53 IS - 3 N2 - BACKGROUND: The recent in vitro demonstration that inositol hexaphosphate-loaded red blood cells (IHP-RBCs) may reduce the risks of sickling of sickle RBCs (SS RBCs) exposed to hypoxia make these modified RBCs potentially useful in transfused sickle cell anemia (SCA) patients. STUDY DESIGN AND METHODS: Hemorheologic properties of IHP-RBCs, normal RBCs (AA RBCs), SS RBCs, SS RBCs plus AA RBCs, and SS RBCs plus IHP-RBCs were compared under normoxia and/or after hypoxic challenges. RESULTS: Although IHP-RBCs have reduced deformability compared with SS RBCs or AA RBCs, IHP-RBCs exhibited lower aggregability than AA RBCs and SS RBCs and, when mixed with SS RBCs, the aggregation level was below the one of SS RBCs alone or SS RBCs plus AA RBCs. Blood viscosity of SS RBC plus IHP-RBC suspension was lower than the viscosity of SS RBCs alone and greater than viscosity of SS RBCs plus AA RBCs. The hypoxic challenge was detrimental for deformability and viscosity of SS RBCs alone or SS plus AA RBC suspension but not for SS plus IHP-RBC suspension. CONCLUSION: Our results support the fact that IHP-RBCs could be useful in SCA by decreasing RBC aggregation and blunting the adverse effects of hypoxia on RBC deformability and blood viscosity. SN - 1537-2995 UR - https://www.unboundmedicine.com/medline/citation/22804873/Effect_of_inositol_hexaphosphate_loaded_red_blood_cells__RBCs__on_the_rheology_of_sickle_RBCs_ L2 - https://doi.org/10.1111/j.1537-2995.2012.03779.x DB - PRIME DP - Unbound Medicine ER -