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Control of hydroxyproline catabolism in Sinorhizobium meliloti.
Mol Microbiol 2012; 85(6):1133-47MM

Abstract

Hydroxyproline (Hyp) in decaying organic matter is a rich source of carbon and nitrogen for microorganisms. A bacterial pathway for Hyp catabolism is known; however, genes and function relationships are not established. In the pathway, trans-4-hydroxy-L-proline (4-L-Hyp) is epimerized to cis-4-hydroxy-D-proline (4-D-Hyp), and then, in three enzymatic reactions, the D-isomer is converted via Δ-pyrroline-4-hydroxy-2-carboxylate (HPC) and α-ketoglutarate semialdehyde (KGSA) to α-ketoglutarate (KG). Here a transcriptional analysis of cells growing on 4-L-Hyp, and the regulation and functions of genes from a Hyp catabolism locus of the legume endosymbiont Sinorhizobium meliloti are reported. Fourteen hydroxyproline catabolism genes (hyp), in five transcripts hypR, hypD, hypH, hypST and hypMNPQO(RE)XYZ, were negatively regulated by hypR. hypRE was shown to encode 4-hydroxyproline 2-epimerase and a hypRE mutant grew with 4-D-Hyp but not 4-L-Hyp. hypO, hypD and hypH are predicted to encode 4-D-Hyp oxidase, HPC deaminase and α-KGSA dehydrogenase respectively. The functions for hypS, hypT, hypX, hypY and hypZ remain to be determined. The data suggest 4-Hyp is converted to the tricarboxylic acid cycle intermediate α-ketoglutarate via the pathway established biochemically for Pseudomonas. This report describes the first molecular characterization of a Hyp catabolism locus.

Authors+Show Affiliations

Center for Environmental Genomics, Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22804907

Citation

White, Catharine E., et al. "Control of Hydroxyproline Catabolism in Sinorhizobium Meliloti." Molecular Microbiology, vol. 85, no. 6, 2012, pp. 1133-47.
White CE, Gavina JM, Morton R, et al. Control of hydroxyproline catabolism in Sinorhizobium meliloti. Mol Microbiol. 2012;85(6):1133-47.
White, C. E., Gavina, J. M., Morton, R., Britz-McKibbin, P., & Finan, T. M. (2012). Control of hydroxyproline catabolism in Sinorhizobium meliloti. Molecular Microbiology, 85(6), pp. 1133-47. doi:10.1111/j.1365-2958.2012.08164.x.
White CE, et al. Control of Hydroxyproline Catabolism in Sinorhizobium Meliloti. Mol Microbiol. 2012;85(6):1133-47. PubMed PMID: 22804907.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Control of hydroxyproline catabolism in Sinorhizobium meliloti. AU - White,Catharine E, AU - Gavina,Jennilee M A, AU - Morton,Richard, AU - Britz-McKibbin,Philip, AU - Finan,Turlough M, Y1 - 2012/07/27/ PY - 2012/7/19/entrez PY - 2012/7/19/pubmed PY - 2013/1/24/medline SP - 1133 EP - 47 JF - Molecular microbiology JO - Mol. Microbiol. VL - 85 IS - 6 N2 - Hydroxyproline (Hyp) in decaying organic matter is a rich source of carbon and nitrogen for microorganisms. A bacterial pathway for Hyp catabolism is known; however, genes and function relationships are not established. In the pathway, trans-4-hydroxy-L-proline (4-L-Hyp) is epimerized to cis-4-hydroxy-D-proline (4-D-Hyp), and then, in three enzymatic reactions, the D-isomer is converted via Δ-pyrroline-4-hydroxy-2-carboxylate (HPC) and α-ketoglutarate semialdehyde (KGSA) to α-ketoglutarate (KG). Here a transcriptional analysis of cells growing on 4-L-Hyp, and the regulation and functions of genes from a Hyp catabolism locus of the legume endosymbiont Sinorhizobium meliloti are reported. Fourteen hydroxyproline catabolism genes (hyp), in five transcripts hypR, hypD, hypH, hypST and hypMNPQO(RE)XYZ, were negatively regulated by hypR. hypRE was shown to encode 4-hydroxyproline 2-epimerase and a hypRE mutant grew with 4-D-Hyp but not 4-L-Hyp. hypO, hypD and hypH are predicted to encode 4-D-Hyp oxidase, HPC deaminase and α-KGSA dehydrogenase respectively. The functions for hypS, hypT, hypX, hypY and hypZ remain to be determined. The data suggest 4-Hyp is converted to the tricarboxylic acid cycle intermediate α-ketoglutarate via the pathway established biochemically for Pseudomonas. This report describes the first molecular characterization of a Hyp catabolism locus. SN - 1365-2958 UR - https://www.unboundmedicine.com/medline/citation/22804907/Control_of_hydroxyproline_catabolism_in_Sinorhizobium_meliloti L2 - https://doi.org/10.1111/j.1365-2958.2012.08164.x DB - PRIME DP - Unbound Medicine ER -