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[The effects of Nrf2 gene expression induced by RU486 at different doses on A549 cell damage induced by paraquat].

Abstract

OBJECTIVE

To observe the effects of Nrf2 gene expression induced by RU486 at different doses on A549 cell damage induced by paraquat (PQ).

METHODS

After A549 cells transfected with Ad-RUNrf2 were treated by RU486 at the doses of 10(-10), 10(-9), 10(-8) and 10(-7) mol/L for 6 h, A549 cell cultures were exposed to 10(-3) mol/L of PQ for 48 h. Then qRT-PCR and EMSA assays were used to detect the expression of Nrf2 gene, and qRT-PCR and ELISA assays were utilized to measure the effects of Nrf2 gene on the expression of the inflammatory cytokines IL-6, IL-10 and TNF-α, apoptotic factors Caspase-3, Caspase-9 and Cytochrome C. The oxidation factors (CAT and MDA protein contents) were observed by Chemical Colorimetric Analysis.

RESULTS

Nrf2 gene relative expression and protein contents increased with RU486 concentrations, and the above expression was the highest when the concentration of RU486 was 10(-7) mol/L, which was significantly higher than those in control and PQ exposure groups (P < 0.01 or P < 0.05). The relative gene expression and protein expression of IL-6 and TNF-α enhanced with the reduced concentrations of RU486, which were the lowest when RU486 concentration was 10(-7) mol/L, as compared with control and PQ exposure groups (P < 0.01 or P < 0.05), while the change of IL-10 content was the opposite. The relative expression of Caspase3, Caspase9 and Cytochrome C genes also increased with the reduced concentrations of RU486, which were the lowest when RU486 concentration was 10(-7) mol/L, as compared with control and PQ exposure groups (P < 0.01 or P < 0.05). The content of CAT enhanced with RU486 concentration, which was the highest when RU486 concentration was 10(-7) mol/L, as compared with control and PQ exposure groups (P < 0.05). But the change of MDA content was the contrary.

CONCLUSION

Nrf2 expression induced by RU486 can promote the balance of oxidation-antioxidation system in A549 cells and inhibit the inflammation and apoptosis factors, which has a protective effect on A549 cell injury induced by PQ.

Authors+Show Affiliations

Emergency Medical Department, First Affiliated Hospital, Wenzhou Medcial College, Wenzhou, Zhejiang Province 325000, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

chi

PubMed ID

22804935

Citation

Jiang, Xiao-zhen, et al. "[The Effects of Nrf2 Gene Expression Induced By RU486 at Different Doses On A549 Cell Damage Induced By Paraquat]." Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, vol. 30, no. 4, 2012, pp. 268-72.
Jiang XZ, Song Q, Xu XP, et al. [The effects of Nrf2 gene expression induced by RU486 at different doses on A549 cell damage induced by paraquat]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2012;30(4):268-72.
Jiang, X. Z., Song, Q., Xu, X. P., Cai, Q. Q., Hong, G. L., Liang, H., & Lu, Z. Q. (2012). [The effects of Nrf2 gene expression induced by RU486 at different doses on A549 cell damage induced by paraquat]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, 30(4), 268-72.
Jiang XZ, et al. [The Effects of Nrf2 Gene Expression Induced By RU486 at Different Doses On A549 Cell Damage Induced By Paraquat]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2012;30(4):268-72. PubMed PMID: 22804935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The effects of Nrf2 gene expression induced by RU486 at different doses on A549 cell damage induced by paraquat]. AU - Jiang,Xiao-zhen, AU - Song,Qing, AU - Xu,Xiu-ping, AU - Cai,Qi-qi, AU - Hong,Guang-liang, AU - Liang,Huan, AU - Lu,Zhong-qiu, PY - 2012/7/19/entrez PY - 2012/7/19/pubmed PY - 2013/4/23/medline SP - 268 EP - 72 JF - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi VL - 30 IS - 4 N2 - OBJECTIVE: To observe the effects of Nrf2 gene expression induced by RU486 at different doses on A549 cell damage induced by paraquat (PQ). METHODS: After A549 cells transfected with Ad-RUNrf2 were treated by RU486 at the doses of 10(-10), 10(-9), 10(-8) and 10(-7) mol/L for 6 h, A549 cell cultures were exposed to 10(-3) mol/L of PQ for 48 h. Then qRT-PCR and EMSA assays were used to detect the expression of Nrf2 gene, and qRT-PCR and ELISA assays were utilized to measure the effects of Nrf2 gene on the expression of the inflammatory cytokines IL-6, IL-10 and TNF-α, apoptotic factors Caspase-3, Caspase-9 and Cytochrome C. The oxidation factors (CAT and MDA protein contents) were observed by Chemical Colorimetric Analysis. RESULTS: Nrf2 gene relative expression and protein contents increased with RU486 concentrations, and the above expression was the highest when the concentration of RU486 was 10(-7) mol/L, which was significantly higher than those in control and PQ exposure groups (P < 0.01 or P < 0.05). The relative gene expression and protein expression of IL-6 and TNF-α enhanced with the reduced concentrations of RU486, which were the lowest when RU486 concentration was 10(-7) mol/L, as compared with control and PQ exposure groups (P < 0.01 or P < 0.05), while the change of IL-10 content was the opposite. The relative expression of Caspase3, Caspase9 and Cytochrome C genes also increased with the reduced concentrations of RU486, which were the lowest when RU486 concentration was 10(-7) mol/L, as compared with control and PQ exposure groups (P < 0.01 or P < 0.05). The content of CAT enhanced with RU486 concentration, which was the highest when RU486 concentration was 10(-7) mol/L, as compared with control and PQ exposure groups (P < 0.05). But the change of MDA content was the contrary. CONCLUSION: Nrf2 expression induced by RU486 can promote the balance of oxidation-antioxidation system in A549 cells and inhibit the inflammation and apoptosis factors, which has a protective effect on A549 cell injury induced by PQ. SN - 1001-9391 UR - https://www.unboundmedicine.com/medline/citation/22804935/[The_effects_of_Nrf2_gene_expression_induced_by_RU486_at_different_doses_on_A549_cell_damage_induced_by_paraquat]_ L2 - https://antibodies.cancer.gov/detail/CPTC-IL6-1 DB - PRIME DP - Unbound Medicine ER -