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Hepatoprotective and antioxidant activity of methanolic extract of flowers of Nerium oleander against CCl4-induced liver injury in rats.
Asian Pac J Trop Med. 2012 Sep; 5(9):677-85.AP

Abstract

OBJECTIVE

To investigate the antioxidant and hepatoprotective activity of methanolic flower extract of Nerium oleander against CCl(4)-induced hepatotoxicity in rats.

METHODS

In vitro antioxidant activity of methanolic extract of flowers of Nerium oleander (MENO-F) was evaluated by various assays, including reducing power, lipid peroxidation, DPPH, ABTS, superoxide anion, hydroxyl radicals and metal chelation. The hepatoprotective and in vivo antioxidant activity of MENO-F were evaluated against CCl(4)-induced hepatic damage in rats. The MENO-F at dose of 100, 200 and 400 mg/kg were administered orally once daily for seven days. Serum enzymatic levels of serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP) and total bilirubin were estimated along with estimation of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in liver tissues. Further histopathological examination of the liver sections was carried out to support the induction of hepatotoxicity and hepatoprotective efficacy.

RESULTS

The extract showed potent activities on reducing power, lipid peroxide, DPPH, ABTS, superoxide anion, hydroxyl radical and metal chelation. The substantially elevated serum enzymatic levels of AST, ALT, ALP and total bilirubin were found to be restored towards normalization significantly by the MENO-F in a dose dependent manner with maximum hepatoprotection at 400 mg/kg dose level. The histopathological observations supported the biochemical evidences of hepatoprotection. Elevated level of SOD and decreased level of MDA further strengthen the hepatoprotective observations.

CONCLUSIONS

The results of the present study strongly reveal that MENO-F has potent antioxidant activity and hepatoprotective activity against CCl(4)-induced hepatic damage in experimental animals.

Authors+Show Affiliations

Pharmacology & Toxicology Research Lab, Department of Pharmacology, ASBASJSM College of Pharmacy, Bela, Punjab, India.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22805717

Citation

Singhal, Kumar Gaurav, and Ghanshyam Das Gupta. "Hepatoprotective and Antioxidant Activity of Methanolic Extract of Flowers of Nerium Oleander Against CCl4-induced Liver Injury in Rats." Asian Pacific Journal of Tropical Medicine, vol. 5, no. 9, 2012, pp. 677-85.
Singhal KG, Gupta GD. Hepatoprotective and antioxidant activity of methanolic extract of flowers of Nerium oleander against CCl4-induced liver injury in rats. Asian Pac J Trop Med. 2012;5(9):677-85.
Singhal, K. G., & Gupta, G. D. (2012). Hepatoprotective and antioxidant activity of methanolic extract of flowers of Nerium oleander against CCl4-induced liver injury in rats. Asian Pacific Journal of Tropical Medicine, 5(9), 677-85. https://doi.org/10.1016/S1995-7645(12)60106-0
Singhal KG, Gupta GD. Hepatoprotective and Antioxidant Activity of Methanolic Extract of Flowers of Nerium Oleander Against CCl4-induced Liver Injury in Rats. Asian Pac J Trop Med. 2012;5(9):677-85. PubMed PMID: 22805717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective and antioxidant activity of methanolic extract of flowers of Nerium oleander against CCl4-induced liver injury in rats. AU - Singhal,Kumar Gaurav, AU - Gupta,Ghanshyam Das, PY - 2012/01/11/received PY - 2012/03/15/revised PY - 2012/05/15/accepted PY - 2012/7/19/entrez PY - 2012/7/19/pubmed PY - 2012/10/18/medline SP - 677 EP - 85 JF - Asian Pacific journal of tropical medicine JO - Asian Pac J Trop Med VL - 5 IS - 9 N2 - OBJECTIVE: To investigate the antioxidant and hepatoprotective activity of methanolic flower extract of Nerium oleander against CCl(4)-induced hepatotoxicity in rats. METHODS: In vitro antioxidant activity of methanolic extract of flowers of Nerium oleander (MENO-F) was evaluated by various assays, including reducing power, lipid peroxidation, DPPH, ABTS, superoxide anion, hydroxyl radicals and metal chelation. The hepatoprotective and in vivo antioxidant activity of MENO-F were evaluated against CCl(4)-induced hepatic damage in rats. The MENO-F at dose of 100, 200 and 400 mg/kg were administered orally once daily for seven days. Serum enzymatic levels of serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP) and total bilirubin were estimated along with estimation of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in liver tissues. Further histopathological examination of the liver sections was carried out to support the induction of hepatotoxicity and hepatoprotective efficacy. RESULTS: The extract showed potent activities on reducing power, lipid peroxide, DPPH, ABTS, superoxide anion, hydroxyl radical and metal chelation. The substantially elevated serum enzymatic levels of AST, ALT, ALP and total bilirubin were found to be restored towards normalization significantly by the MENO-F in a dose dependent manner with maximum hepatoprotection at 400 mg/kg dose level. The histopathological observations supported the biochemical evidences of hepatoprotection. Elevated level of SOD and decreased level of MDA further strengthen the hepatoprotective observations. CONCLUSIONS: The results of the present study strongly reveal that MENO-F has potent antioxidant activity and hepatoprotective activity against CCl(4)-induced hepatic damage in experimental animals. SN - 2352-4146 UR - https://www.unboundmedicine.com/medline/citation/22805717/Hepatoprotective_and_antioxidant_activity_of_methanolic_extract_of_flowers_of_Nerium_oleander_against_CCl4_induced_liver_injury_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1995-7645(12)60106-0 DB - PRIME DP - Unbound Medicine ER -