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Influence of oxidative and nitrosative stress on accumulation of diphosphate intermediates of the non-mevalonate pathway of isoprenoid biosynthesis in corynebacteria and mycobacteria.
Biochemistry (Mosc). 2012 Apr; 77(4):362-71.B

Abstract

Artificial generation of oxygen superoxide radicals in actively growing cultures of Mycobacterium tuberculosis, Myc. smegmatis, and Corynebacterium ammoniagenes is followed by accumulation in the bacterial cells of substantial amounts of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate (MEcDP) - an intermediate of the non-mevalonate pathway of isoprenoid biosynthesis (MEP) - most possibly due to the interaction of the oxygen radicals with the 4Fe-4S group in the active center and inhibition of the enzyme (E)-4-oxy-3-methylbut-2-enyl diphosphate synthase (IspG). Cadmium ions known to inhibit IspG enzyme in chloroplasts (Rivasseau, C., Seemann, M., Boisson, A. M., Streb, P., Gout, E., Douce, R., Rohmer, M., and Bligny, R. (2009) Plant Cell Environ., 32, 82-92), when added to culture of Myc. smegmatis, substantially increase accumulation of MEcDP induced by oxidative stress with no accumulation of other organic phosphate intermediates in the cell. Corynebacterium ammoniagenes'', well-known for its ability to synthesize large amounts of MEcDP, was also shown to accumulate this unique cyclodiphosphate in actively growing culture when NO at low concentration is artificially generated in the medium. A possible role of the MEP-pathway of isoprenoid biosynthesis and a role of its central intermediate MEcDP in bacterial response to nitrosative and oxidative stress is discussed.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Artsatbanov VY
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, 119071 Moscow, Russia.
Vostroknutova GN
No affiliation info available
Shleeva MO
No affiliation info available
Goncharenko AV
No affiliation info available
Zinin AI
No affiliation info available
Ostrovsky DN
No affiliation info available
Kapreliants AS
No affiliation info available

MeSH

Biosynthetic PathwaysCorynebacteriumDiphosphatesMevalonic AcidMycobacterium smegmatisMycobacterium tuberculosisOxidative StressReactive Nitrogen SpeciesSuperoxidesTerpenes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22809155

Citation

Artsatbanov, V Yu, et al. "Influence of Oxidative and Nitrosative Stress On Accumulation of Diphosphate Intermediates of the Non-mevalonate Pathway of Isoprenoid Biosynthesis in Corynebacteria and Mycobacteria." Biochemistry. Biokhimiia, vol. 77, no. 4, 2012, pp. 362-71.
Artsatbanov VY, Vostroknutova GN, Shleeva MO, et al. Influence of oxidative and nitrosative stress on accumulation of diphosphate intermediates of the non-mevalonate pathway of isoprenoid biosynthesis in corynebacteria and mycobacteria. Biochemistry (Mosc). 2012;77(4):362-71.
Artsatbanov, V. Y., Vostroknutova, G. N., Shleeva, M. O., Goncharenko, A. V., Zinin, A. I., Ostrovsky, D. N., & Kapreliants, A. S. (2012). Influence of oxidative and nitrosative stress on accumulation of diphosphate intermediates of the non-mevalonate pathway of isoprenoid biosynthesis in corynebacteria and mycobacteria. Biochemistry. Biokhimiia, 77(4), 362-71. https://doi.org/10.1134/S0006297912040074
Artsatbanov VY, et al. Influence of Oxidative and Nitrosative Stress On Accumulation of Diphosphate Intermediates of the Non-mevalonate Pathway of Isoprenoid Biosynthesis in Corynebacteria and Mycobacteria. Biochemistry (Mosc). 2012;77(4):362-71. PubMed PMID: 22809155.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of oxidative and nitrosative stress on accumulation of diphosphate intermediates of the non-mevalonate pathway of isoprenoid biosynthesis in corynebacteria and mycobacteria. AU - Artsatbanov,V Yu, AU - Vostroknutova,G N, AU - Shleeva,M O, AU - Goncharenko,A V, AU - Zinin,A I, AU - Ostrovsky,D N, AU - Kapreliants,A S, PY - 2012/7/20/entrez PY - 2012/7/20/pubmed PY - 2012/12/10/medline SP - 362 EP - 71 JF - Biochemistry. Biokhimiia JO - Biochemistry (Mosc) VL - 77 IS - 4 N2 - Artificial generation of oxygen superoxide radicals in actively growing cultures of Mycobacterium tuberculosis, Myc. smegmatis, and Corynebacterium ammoniagenes is followed by accumulation in the bacterial cells of substantial amounts of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate (MEcDP) - an intermediate of the non-mevalonate pathway of isoprenoid biosynthesis (MEP) - most possibly due to the interaction of the oxygen radicals with the 4Fe-4S group in the active center and inhibition of the enzyme (E)-4-oxy-3-methylbut-2-enyl diphosphate synthase (IspG). Cadmium ions known to inhibit IspG enzyme in chloroplasts (Rivasseau, C., Seemann, M., Boisson, A. M., Streb, P., Gout, E., Douce, R., Rohmer, M., and Bligny, R. (2009) Plant Cell Environ., 32, 82-92), when added to culture of Myc. smegmatis, substantially increase accumulation of MEcDP induced by oxidative stress with no accumulation of other organic phosphate intermediates in the cell. Corynebacterium ammoniagenes'', well-known for its ability to synthesize large amounts of MEcDP, was also shown to accumulate this unique cyclodiphosphate in actively growing culture when NO at low concentration is artificially generated in the medium. A possible role of the MEP-pathway of isoprenoid biosynthesis and a role of its central intermediate MEcDP in bacterial response to nitrosative and oxidative stress is discussed. SN - 1608-3040 UR - https://www.unboundmedicine.com/medline/citation/22809155/Influence_of_oxidative_and_nitrosative_stress_on_accumulation_of_diphosphate_intermediates_of_the_non_mevalonate_pathway_of_isoprenoid_biosynthesis_in_corynebacteria_and_mycobacteria_ DB - PRIME DP - Unbound Medicine ER -
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