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The polyphenol-rich extracts from black chokeberry and grape seeds impair changes in the platelet adhesion and aggregation induced by a model of hyperhomocysteinemia.
Eur J Nutr. 2013 Apr; 52(3):1049-57.EJ

Abstract

OBJECTIVE

The mechanism action of the polyphenol-rich extracts from berries of Aronia melanocarpa (black chokeberry) and from grape seeds in the defence against homocysteine (Hcy) and its derivatives action in blood platelets is still unknown. In this study, the influence of the aronia extract and grape seeds extract (GSE) on the platelet adhesion to collagen and fibrinogen and the platelet aggregation during a model of hyperhomocysteinemia was investigated. The aim of our study in vitro was also to investigate superoxide anion radicals (O₂⁻•) production after incubation of platelets with Hcy, HTL and the aronia extract and GSE during a model of hyperhomocysteinemia (induced by reduced form of homocysteine at final dose of 100 μM) and the most reactive form of Hcy--its cyclic thioester, homocysteine thiolactone (HTL, 1 μM). Moreover, the additional aim of our study was also to establish and compare the influence of the aronia extract, GSE and resveratrol (3,4',5-trihydroxystilben), a phenolic compound, which has been supposed to be beneficial for the prevention of cardiovascular events, on selected steps of platelet activation.

METHODS

The effects of tested extracts on adhesion of blood platelets to collagen and fibrinogen were determined according to Tuszynski and Murphy. The platelet aggregation was determined by turbidimetry method using a Chrono-log Lumi-aggregometer.

RESULTS

We have observed that HTL, like its precursor-Hcy stimulated the generation of O₂⁻• (measured by the superoxide dismutase-inhibitable reduction of cytochrome c) in platelets and caused an augmentation of the platelet adhesion and aggregation induced by the strong physiological agonist-thrombin. Our present results in vitro also demonstrated that the aronia extract and grape seeds extract reduced the toxicity action of Hcy and HTL on blood platelet adhesion to collagen and fibrinogen, the platelet aggregation and superoxide anion radicals production in platelets, suggesting its potential protective effects on hemostasis during hyperhomocysteinemia.

CONCLUSION

In the comparative studies, the aronia extract was found to be more effective antiplatelet factors, than GSE or resveratrol during a model of hyperhomocysteinemia. It gives hopes for development of diet supplements, which may be important during hyperhomocysteinemia.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Malinowska J
Department of General Biochemistry, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland. malinowskaj@hotmail.com
Oleszek W
No affiliation info available
Stochmal A
No affiliation info available
Olas B
No affiliation info available

MeSH

AdultAntioxidantsBlood PlateletsCardiovascular DiseasesCollagenDietary SupplementsFibrinogenFruitGrape Seed ExtractHomocysteineHumansHyperhomocysteinemiaPhotiniaPlant ExtractsPlatelet ActivationPlatelet Aggregation InhibitorsPolyphenolsSeedsSuperoxidesVitisYoung Adult

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22810463

Citation

Malinowska, Joanna, et al. "The Polyphenol-rich Extracts From Black Chokeberry and Grape Seeds Impair Changes in the Platelet Adhesion and Aggregation Induced By a Model of Hyperhomocysteinemia." European Journal of Nutrition, vol. 52, no. 3, 2013, pp. 1049-57.
Malinowska J, Oleszek W, Stochmal A, et al. The polyphenol-rich extracts from black chokeberry and grape seeds impair changes in the platelet adhesion and aggregation induced by a model of hyperhomocysteinemia. Eur J Nutr. 2013;52(3):1049-57.
Malinowska, J., Oleszek, W., Stochmal, A., & Olas, B. (2013). The polyphenol-rich extracts from black chokeberry and grape seeds impair changes in the platelet adhesion and aggregation induced by a model of hyperhomocysteinemia. European Journal of Nutrition, 52(3), 1049-57. https://doi.org/10.1007/s00394-012-0411-8
Malinowska J, et al. The Polyphenol-rich Extracts From Black Chokeberry and Grape Seeds Impair Changes in the Platelet Adhesion and Aggregation Induced By a Model of Hyperhomocysteinemia. Eur J Nutr. 2013;52(3):1049-57. PubMed PMID: 22810463.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The polyphenol-rich extracts from black chokeberry and grape seeds impair changes in the platelet adhesion and aggregation induced by a model of hyperhomocysteinemia. AU - Malinowska,Joanna, AU - Oleszek,Wieslaw, AU - Stochmal,Anna, AU - Olas,Beata, Y1 - 2012/07/19/ PY - 2012/04/04/received PY - 2012/06/22/accepted PY - 2012/7/20/entrez PY - 2012/7/20/pubmed PY - 2013/10/22/medline SP - 1049 EP - 57 JF - European journal of nutrition JO - Eur J Nutr VL - 52 IS - 3 N2 - OBJECTIVE: The mechanism action of the polyphenol-rich extracts from berries of Aronia melanocarpa (black chokeberry) and from grape seeds in the defence against homocysteine (Hcy) and its derivatives action in blood platelets is still unknown. In this study, the influence of the aronia extract and grape seeds extract (GSE) on the platelet adhesion to collagen and fibrinogen and the platelet aggregation during a model of hyperhomocysteinemia was investigated. The aim of our study in vitro was also to investigate superoxide anion radicals (O₂⁻•) production after incubation of platelets with Hcy, HTL and the aronia extract and GSE during a model of hyperhomocysteinemia (induced by reduced form of homocysteine at final dose of 100 μM) and the most reactive form of Hcy--its cyclic thioester, homocysteine thiolactone (HTL, 1 μM). Moreover, the additional aim of our study was also to establish and compare the influence of the aronia extract, GSE and resveratrol (3,4',5-trihydroxystilben), a phenolic compound, which has been supposed to be beneficial for the prevention of cardiovascular events, on selected steps of platelet activation. METHODS: The effects of tested extracts on adhesion of blood platelets to collagen and fibrinogen were determined according to Tuszynski and Murphy. The platelet aggregation was determined by turbidimetry method using a Chrono-log Lumi-aggregometer. RESULTS: We have observed that HTL, like its precursor-Hcy stimulated the generation of O₂⁻• (measured by the superoxide dismutase-inhibitable reduction of cytochrome c) in platelets and caused an augmentation of the platelet adhesion and aggregation induced by the strong physiological agonist-thrombin. Our present results in vitro also demonstrated that the aronia extract and grape seeds extract reduced the toxicity action of Hcy and HTL on blood platelet adhesion to collagen and fibrinogen, the platelet aggregation and superoxide anion radicals production in platelets, suggesting its potential protective effects on hemostasis during hyperhomocysteinemia. CONCLUSION: In the comparative studies, the aronia extract was found to be more effective antiplatelet factors, than GSE or resveratrol during a model of hyperhomocysteinemia. It gives hopes for development of diet supplements, which may be important during hyperhomocysteinemia. SN - 1436-6215 UR - https://www.unboundmedicine.com/medline/citation/22810463/The_polyphenol_rich_extracts_from_black_chokeberry_and_grape_seeds_impair_changes_in_the_platelet_adhesion_and_aggregation_induced_by_a_model_of_hyperhomocysteinemia_ L2 - https://dx.doi.org/10.1007/s00394-012-0411-8 DB - PRIME DP - Unbound Medicine ER -