Tags

Type your tag names separated by a space and hit enter

Rapid and sensitive detection of auxins and flavonoids in plant samples by high-performance liquid chromatography coupled with tandem mass spectrometry.
J Sep Sci. 2012 Oct; 35(19):2559-66.JS

Abstract

Simultaneous determination of indole-3-acetic acid and methyl indole-3-acetic acid ester in small amounts of plant tissue is essential for elucidating their mutual transformation mechanism and the in vivo function of methyl indole-3-acetic acid ester. Rapid quantification of flavonoids in the same sample is important for clarifying their roles in the transport of auxins and other phytohormones. Herein, we describe a simple method for the simultaneous determination of indole-3-acetic acid and its methyl ester in the roots of the Arabidopsis thaliana seedlings and a protocol for the rapid extraction and quantification of quercetin and kaempferol in these seedlings. High-performance liquid chromatography coupled with electrospray ionization time-of-flight tandem mass spectrometry was used for the detection of all the compounds. Negative data for indole-3-acetic acid and positive data for methyl indole-3-acetic acid ester were collected in two successive files with a single injection of the extracted sample. Under optimized conditions, the limit of detection for the four compounds was 2 ng/mL for indole-3-acetic acid, 0.5 ng/mL for methyl indole-3-acetic acid ester, 5 ng/mL for quercetin, and 1 ng/mL for kaempferol, respectively. Because of the high sensitivity of the assay, only 2-10 mg of the plant material was required to obtain quantitative results.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Liang Y
Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
Zhu X
No affiliation info available
Wu T
No affiliation info available
Zhao M
No affiliation info available
Liu H
No affiliation info available

MeSH

ArabidopsisChromatography, High Pressure LiquidIndoleacetic AcidsPlant ExtractsPlant Growth RegulatorsQuercetinSensitivity and SpecificityTandem Mass Spectrometry

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22815051

Citation

Liang, Yuan, et al. "Rapid and Sensitive Detection of Auxins and Flavonoids in Plant Samples By High-performance Liquid Chromatography Coupled With Tandem Mass Spectrometry." Journal of Separation Science, vol. 35, no. 19, 2012, pp. 2559-66.
Liang Y, Zhu X, Wu T, et al. Rapid and sensitive detection of auxins and flavonoids in plant samples by high-performance liquid chromatography coupled with tandem mass spectrometry. J Sep Sci. 2012;35(19):2559-66.
Liang, Y., Zhu, X., Wu, T., Zhao, M., & Liu, H. (2012). Rapid and sensitive detection of auxins and flavonoids in plant samples by high-performance liquid chromatography coupled with tandem mass spectrometry. Journal of Separation Science, 35(19), 2559-66. https://doi.org/10.1002/jssc.201200418
Liang Y, et al. Rapid and Sensitive Detection of Auxins and Flavonoids in Plant Samples By High-performance Liquid Chromatography Coupled With Tandem Mass Spectrometry. J Sep Sci. 2012;35(19):2559-66. PubMed PMID: 22815051.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and sensitive detection of auxins and flavonoids in plant samples by high-performance liquid chromatography coupled with tandem mass spectrometry. AU - Liang,Yuan, AU - Zhu,Xiaocui, AU - Wu,Tongbo, AU - Zhao,Meiping, AU - Liu,Huwei, Y1 - 2012/07/19/ PY - 2012/04/27/received PY - 2012/05/19/revised PY - 2012/05/23/accepted PY - 2012/7/21/entrez PY - 2012/7/21/pubmed PY - 2013/2/8/medline SP - 2559 EP - 66 JF - Journal of separation science JO - J Sep Sci VL - 35 IS - 19 N2 - Simultaneous determination of indole-3-acetic acid and methyl indole-3-acetic acid ester in small amounts of plant tissue is essential for elucidating their mutual transformation mechanism and the in vivo function of methyl indole-3-acetic acid ester. Rapid quantification of flavonoids in the same sample is important for clarifying their roles in the transport of auxins and other phytohormones. Herein, we describe a simple method for the simultaneous determination of indole-3-acetic acid and its methyl ester in the roots of the Arabidopsis thaliana seedlings and a protocol for the rapid extraction and quantification of quercetin and kaempferol in these seedlings. High-performance liquid chromatography coupled with electrospray ionization time-of-flight tandem mass spectrometry was used for the detection of all the compounds. Negative data for indole-3-acetic acid and positive data for methyl indole-3-acetic acid ester were collected in two successive files with a single injection of the extracted sample. Under optimized conditions, the limit of detection for the four compounds was 2 ng/mL for indole-3-acetic acid, 0.5 ng/mL for methyl indole-3-acetic acid ester, 5 ng/mL for quercetin, and 1 ng/mL for kaempferol, respectively. Because of the high sensitivity of the assay, only 2-10 mg of the plant material was required to obtain quantitative results. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/22815051/Rapid_and_sensitive_detection_of_auxins_and_flavonoids_in_plant_samples_by_high_performance_liquid_chromatography_coupled_with_tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/jssc.201200418 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓4]

Related Citations

More