Tags

Type your tag names separated by a space and hit enter

The antioxidant and anticancer effects of wild carrot oil extract.
Phytother Res. 2013 May; 27(5):737-44.PR

Abstract

Daucus carota L. ssp. carota (Apiacea) is used in traditional medicine in Lebanon and in different regions throughout the world. The present study investigates the in vitro anticancer activities of Daucus carota oil extract (DCOE) on four human cancer cell lines as well as its in vitro antioxidant activity. DCOE was extracted from the dried umbels with 50:50 acetone-methanol. The oil extract was analyzed by gas chromatography-mass spectrometry and screened for its antioxidant properties in vitro using 1,1-diphenyl-2-picryl hydrazyl free radical scavenging assay (DPPH), ferrous ion chelating assay (FIC) and the ferric reducing antioxidant power assay (FRAP). The anticancer activity of the oil extract against human colon (HT-29, Caco-2) and breast (MCF-7, MDA-MB-231) cancer cell lines was evaluated using the trypan blue exclusion method and the WST-1 cell proliferation assay. DCOE exhibited antioxidant activity in all assays used. The FRAP value was 164 ± 5.5 µmol FeSO4 /g, and the IC50 values for DPPH and FIC assays were 2.1 ± 0.03 mg/ml and 0.43 ± 0.02 mg/ml, respectively. Also, DCOE demonstrated a significant increase in cell death and decrease in cell proliferation. The effect of DCOE on the cell lines exhibited time and dose-dependent responses. The present study established that DCOE possesses both antioxidant and promising anticancer activities.

Authors+Show Affiliations

Faculty of Health and Medical Sciences, Department of Microbial and Cellular Sciences, University of Surrey, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22815230

Citation

Shebaby, Wassim Nasri, et al. "The Antioxidant and Anticancer Effects of Wild Carrot Oil Extract." Phytotherapy Research : PTR, vol. 27, no. 5, 2013, pp. 737-44.
Shebaby WN, El-Sibai M, Smith KB, et al. The antioxidant and anticancer effects of wild carrot oil extract. Phytother Res. 2013;27(5):737-44.
Shebaby, W. N., El-Sibai, M., Smith, K. B., Karam, M. C., Mroueh, M., & Daher, C. F. (2013). The antioxidant and anticancer effects of wild carrot oil extract. Phytotherapy Research : PTR, 27(5), 737-44. https://doi.org/10.1002/ptr.4776
Shebaby WN, et al. The Antioxidant and Anticancer Effects of Wild Carrot Oil Extract. Phytother Res. 2013;27(5):737-44. PubMed PMID: 22815230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The antioxidant and anticancer effects of wild carrot oil extract. AU - Shebaby,Wassim Nasri, AU - El-Sibai,Mirvat, AU - Smith,Kikki Bodman-, AU - Karam,Marc Christoph, AU - Mroueh,Mohamad, AU - Daher,Costantine F, Y1 - 2012/07/20/ PY - 2011/09/29/received PY - 2012/06/08/revised PY - 2012/06/09/accepted PY - 2012/7/21/entrez PY - 2012/7/21/pubmed PY - 2013/8/2/medline SP - 737 EP - 44 JF - Phytotherapy research : PTR JO - Phytother Res VL - 27 IS - 5 N2 - Daucus carota L. ssp. carota (Apiacea) is used in traditional medicine in Lebanon and in different regions throughout the world. The present study investigates the in vitro anticancer activities of Daucus carota oil extract (DCOE) on four human cancer cell lines as well as its in vitro antioxidant activity. DCOE was extracted from the dried umbels with 50:50 acetone-methanol. The oil extract was analyzed by gas chromatography-mass spectrometry and screened for its antioxidant properties in vitro using 1,1-diphenyl-2-picryl hydrazyl free radical scavenging assay (DPPH), ferrous ion chelating assay (FIC) and the ferric reducing antioxidant power assay (FRAP). The anticancer activity of the oil extract against human colon (HT-29, Caco-2) and breast (MCF-7, MDA-MB-231) cancer cell lines was evaluated using the trypan blue exclusion method and the WST-1 cell proliferation assay. DCOE exhibited antioxidant activity in all assays used. The FRAP value was 164 ± 5.5 µmol FeSO4 /g, and the IC50 values for DPPH and FIC assays were 2.1 ± 0.03 mg/ml and 0.43 ± 0.02 mg/ml, respectively. Also, DCOE demonstrated a significant increase in cell death and decrease in cell proliferation. The effect of DCOE on the cell lines exhibited time and dose-dependent responses. The present study established that DCOE possesses both antioxidant and promising anticancer activities. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/22815230/The_antioxidant_and_anticancer_effects_of_wild_carrot_oil_extract_ L2 - https://doi.org/10.1002/ptr.4776 DB - PRIME DP - Unbound Medicine ER -