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Imbalanced shift of cytokine expression between T helper 1 and T helper 2 (Th1/Th2) in intestinal mucosa of patients with post-infectious irritable bowel syndrome.
BMC Gastroenterol. 2012 Jul 20; 12:91.BG

Abstract

BACKGROUND

Irritable bowel syndrome (IBS) is a common functional bowel disorder. The post-infectious IBS (PI-IBS) occurs in IBS patients with a history of intestinal infection preceding the onset of symptoms. However, the underlying cause of PI-IBS is not fully understood, and the purpose of this study was to investigate the immune regulatory mechanism of PI-IBS.

METHODS

Participants enrolled in this study were divided into three groups including PI-IBS patients (n = 20), IBS patients without a history of infection (non-PI-IBS, n = 18), and healthy controls (n = 20). The expression levels of the Th1-derived cytokines IFN-γ and IL-12, and the Th2-derived cytokines IL-4 and IL-10 in the mucosal specimens, and in the ascending colon, the descending colon, and the rectal segments were measured by RT-PCR and western blot.

RESULTS

The IFN-γ mRNA levels in the intestinal mucosa were significantly higher in the PI-IBS group than in the non-PI-IBS or control group (both P < 0.05), but there was no difference between the non-PI-IBS and control groups. A trend toward IFN-γ protein upregulation was found in the PI-IBS group, while the IL-12 and IL-4 mRNA and protein levels were not different between any groups. The IL-10 mRNA and protein levels in the PI-IBS group were both significantly lower than in the non-PI-IBS or control groups (P < 0.05, respectively), but there was no difference between the non-PI-IBS and control groups. There were no differences in the cytokine mRNA and protein levels among the ascending colon, the descending colon, and the rectum of all groups.

CONCLUSIONS

An increase in IFN-γ levels and a decrease in IL-10 levels were found in the intestinal mucosa of PI-IBS patients, suggesting that the infection may affect the Th1/Th2 balance. Thus, the dysregulation of the immune response is likely an important cause of IBS.

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Authors+Show Affiliations

Chen J
National Hepatobiliary & Enteric Surgery Research Center, Ministry of Health, Central South University, Changsha 410008, China. chenji4728@163.com
Zhang Y
No affiliation info available
Deng Z
No affiliation info available

MeSH

AdultCase-Control StudiesColonCytokinesFemaleHumansImmune SystemInterferon-gammaInterleukin-10Interleukin-12Interleukin-4Intestinal DiseasesIntestinal MucosaIrritable Bowel SyndromeMaleMiddle AgedRNA, MessengerTh1 CellsTh2 Cells

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22816602

Citation

Chen, Ji, et al. "Imbalanced Shift of Cytokine Expression Between T Helper 1 and T Helper 2 (Th1/Th2) in Intestinal Mucosa of Patients With Post-infectious Irritable Bowel Syndrome." BMC Gastroenterology, vol. 12, 2012, p. 91.
Chen J, Zhang Y, Deng Z. Imbalanced shift of cytokine expression between T helper 1 and T helper 2 (Th1/Th2) in intestinal mucosa of patients with post-infectious irritable bowel syndrome. BMC Gastroenterol. 2012;12:91.
Chen, J., Zhang, Y., & Deng, Z. (2012). Imbalanced shift of cytokine expression between T helper 1 and T helper 2 (Th1/Th2) in intestinal mucosa of patients with post-infectious irritable bowel syndrome. BMC Gastroenterology, 12, 91. https://doi.org/10.1186/1471-230X-12-91
Chen J, Zhang Y, Deng Z. Imbalanced Shift of Cytokine Expression Between T Helper 1 and T Helper 2 (Th1/Th2) in Intestinal Mucosa of Patients With Post-infectious Irritable Bowel Syndrome. BMC Gastroenterol. 2012 Jul 20;12:91. PubMed PMID: 22816602.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Imbalanced shift of cytokine expression between T helper 1 and T helper 2 (Th1/Th2) in intestinal mucosa of patients with post-infectious irritable bowel syndrome. AU - Chen,Ji, AU - Zhang,Yangde, AU - Deng,Zhansheng, Y1 - 2012/07/20/ PY - 2012/02/29/received PY - 2012/06/29/accepted PY - 2012/7/24/entrez PY - 2012/7/24/pubmed PY - 2013/4/12/medline SP - 91 EP - 91 JF - BMC gastroenterology JO - BMC Gastroenterol VL - 12 N2 - BACKGROUND: Irritable bowel syndrome (IBS) is a common functional bowel disorder. The post-infectious IBS (PI-IBS) occurs in IBS patients with a history of intestinal infection preceding the onset of symptoms. However, the underlying cause of PI-IBS is not fully understood, and the purpose of this study was to investigate the immune regulatory mechanism of PI-IBS. METHODS: Participants enrolled in this study were divided into three groups including PI-IBS patients (n = 20), IBS patients without a history of infection (non-PI-IBS, n = 18), and healthy controls (n = 20). The expression levels of the Th1-derived cytokines IFN-γ and IL-12, and the Th2-derived cytokines IL-4 and IL-10 in the mucosal specimens, and in the ascending colon, the descending colon, and the rectal segments were measured by RT-PCR and western blot. RESULTS: The IFN-γ mRNA levels in the intestinal mucosa were significantly higher in the PI-IBS group than in the non-PI-IBS or control group (both P < 0.05), but there was no difference between the non-PI-IBS and control groups. A trend toward IFN-γ protein upregulation was found in the PI-IBS group, while the IL-12 and IL-4 mRNA and protein levels were not different between any groups. The IL-10 mRNA and protein levels in the PI-IBS group were both significantly lower than in the non-PI-IBS or control groups (P < 0.05, respectively), but there was no difference between the non-PI-IBS and control groups. There were no differences in the cytokine mRNA and protein levels among the ascending colon, the descending colon, and the rectum of all groups. CONCLUSIONS: An increase in IFN-γ levels and a decrease in IL-10 levels were found in the intestinal mucosa of PI-IBS patients, suggesting that the infection may affect the Th1/Th2 balance. Thus, the dysregulation of the immune response is likely an important cause of IBS. SN - 1471-230X UR - https://www.unboundmedicine.com/medline/citation/22816602/Imbalanced_shift_of_cytokine_expression_between_T_helper_1_and_T_helper_2__Th1/Th2__in_intestinal_mucosa_of_patients_with_post_infectious_irritable_bowel_syndrome_ L2 - https://bmcgastroenterol.biomedcentral.com/articles/10.1186/1471-230X-12-91 DB - PRIME DP - Unbound Medicine ER -