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Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum.
Avian Dis. 1990 Oct-Dec; 34(4):861-4.AD

Abstract

Two monoclonal antibodies (MAbs) were evaluated for their ability to serotype 108 isolates of Haemophilus paragallinarum. One MAb (E5C12D10) was raised against a Page serovar A strain and the other (F2E6) against a Page serovar C strain. In both dot blot and hemagglutination-inhibition tests, MAb E5C12D10 recognized the type strains of Page serovar A and Kume serovars A-1, A-2, A-3, and A-4. MAb F2E6 recognized the type strains of Page serovar C and Kume serovars C-1, C-2, and C-3. Neither antibody recognized the type strains of Page serovar B or Kume serovars B-1 and C-4. When evaluated with 97 field isolates in a dot blot test, the MAbs serotyped 81 isolates, which was better than agglutinin typing by the Page scheme (69 isolates serotyped). The field isolates that did not react with the MAbs were either Page serovar B/Kume serovar B-1 (three isolates), Page serovar C/Kume serovar C-4 (12 isolates), or nontypable by either the Page or Kume scheme (one isolate).

Authors+Show Affiliations

Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly, Australia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2282016

Citation

Blackall, P J., et al. "Evaluation of Two Monoclonal Antibodies for Serotyping Haemophilus Paragallinarum." Avian Diseases, vol. 34, no. 4, 1990, pp. 861-4.
Blackall PJ, Yamaguchi T, Iritani Y, et al. Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum. Avian Dis. 1990;34(4):861-4.
Blackall, P. J., Yamaguchi, T., Iritani, Y., & Rogers, D. G. (1990). Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum. Avian Diseases, 34(4), 861-4.
Blackall PJ, et al. Evaluation of Two Monoclonal Antibodies for Serotyping Haemophilus Paragallinarum. Avian Dis. 1990 Oct-Dec;34(4):861-4. PubMed PMID: 2282016.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum. AU - Blackall,P J, AU - Yamaguchi,T, AU - Iritani,Y, AU - Rogers,D G, PY - 1990/10/1/pubmed PY - 1990/10/1/medline PY - 1990/10/1/entrez SP - 861 EP - 4 JF - Avian diseases JO - Avian Dis. VL - 34 IS - 4 N2 - Two monoclonal antibodies (MAbs) were evaluated for their ability to serotype 108 isolates of Haemophilus paragallinarum. One MAb (E5C12D10) was raised against a Page serovar A strain and the other (F2E6) against a Page serovar C strain. In both dot blot and hemagglutination-inhibition tests, MAb E5C12D10 recognized the type strains of Page serovar A and Kume serovars A-1, A-2, A-3, and A-4. MAb F2E6 recognized the type strains of Page serovar C and Kume serovars C-1, C-2, and C-3. Neither antibody recognized the type strains of Page serovar B or Kume serovars B-1 and C-4. When evaluated with 97 field isolates in a dot blot test, the MAbs serotyped 81 isolates, which was better than agglutinin typing by the Page scheme (69 isolates serotyped). The field isolates that did not react with the MAbs were either Page serovar B/Kume serovar B-1 (three isolates), Page serovar C/Kume serovar C-4 (12 isolates), or nontypable by either the Page or Kume scheme (one isolate). SN - 0005-2086 UR - https://www.unboundmedicine.com/medline/citation/2282016/Evaluation_of_two_monoclonal_antibodies_for_serotyping_Haemophilus_paragallinarum_ DB - PRIME DP - Unbound Medicine ER -