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Characterization of two new monoclonal antibodies against Haemophilus paragallinarum serovar C hemagglutinating antigen.
Avian Dis 1990 Oct-Dec; 34(4):922-7AD

Abstract

Two new monoclonal antibodies (MAbs), D6D8D5 and B3E6F9, both directed against Haemophilus paragallinarum serovar C hemagglutinating (HA) antigen, were produced, and characteristics of the MAbs were compared with those of the previously described MAb F2E6 in dot-blot and hemagglutination-inhibition (HI) tests using two representative H. paragallinarum strains each of serovars A, B, and C strains and 55 Japanese serovar C field isolates. MAb D6D8D5 and MAb F2E6 reacted with all serovar C strains and field isolates in the dot-blot test. However, MAb D6D8D5 showed various degrees of inhibition of the HA activity of field isolates. In the enzyme-linked immunosorbent assay-competition test, MAb D6D8D5 did not compete with MAb F2E6. MAb B3E6F9 reacted with strain S1, serovar C but not with strain Modesto, serovar C in both dot-blot and HI tests. Three out of 55 field isolates did not react with MAb B3E6F9. Neither MAb reacted with the serovar A and B strains.

Authors+Show Affiliations

Aburahi Laboratories, Shionogi & Co., Ltd., Shiga, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

2282021

Citation

Yamaguchi, T, et al. "Characterization of Two New Monoclonal Antibodies Against Haemophilus Paragallinarum Serovar C Hemagglutinating Antigen." Avian Diseases, vol. 34, no. 4, 1990, pp. 922-7.
Yamaguchi T, Takigami S, Iritani Y, et al. Characterization of two new monoclonal antibodies against Haemophilus paragallinarum serovar C hemagglutinating antigen. Avian Dis. 1990;34(4):922-7.
Yamaguchi, T., Takigami, S., Iritani, Y., & Hayashi, Y. (1990). Characterization of two new monoclonal antibodies against Haemophilus paragallinarum serovar C hemagglutinating antigen. Avian Diseases, 34(4), pp. 922-7.
Yamaguchi T, et al. Characterization of Two New Monoclonal Antibodies Against Haemophilus Paragallinarum Serovar C Hemagglutinating Antigen. Avian Dis. 1990;34(4):922-7. PubMed PMID: 2282021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of two new monoclonal antibodies against Haemophilus paragallinarum serovar C hemagglutinating antigen. AU - Yamaguchi,T, AU - Takigami,S, AU - Iritani,Y, AU - Hayashi,Y, PY - 1990/10/1/pubmed PY - 1990/10/1/medline PY - 1990/10/1/entrez SP - 922 EP - 7 JF - Avian diseases JO - Avian Dis. VL - 34 IS - 4 N2 - Two new monoclonal antibodies (MAbs), D6D8D5 and B3E6F9, both directed against Haemophilus paragallinarum serovar C hemagglutinating (HA) antigen, were produced, and characteristics of the MAbs were compared with those of the previously described MAb F2E6 in dot-blot and hemagglutination-inhibition (HI) tests using two representative H. paragallinarum strains each of serovars A, B, and C strains and 55 Japanese serovar C field isolates. MAb D6D8D5 and MAb F2E6 reacted with all serovar C strains and field isolates in the dot-blot test. However, MAb D6D8D5 showed various degrees of inhibition of the HA activity of field isolates. In the enzyme-linked immunosorbent assay-competition test, MAb D6D8D5 did not compete with MAb F2E6. MAb B3E6F9 reacted with strain S1, serovar C but not with strain Modesto, serovar C in both dot-blot and HI tests. Three out of 55 field isolates did not react with MAb B3E6F9. Neither MAb reacted with the serovar A and B strains. SN - 0005-2086 UR - https://www.unboundmedicine.com/medline/citation/2282021/Characterization_of_two_new_monoclonal_antibodies_against_Haemophilus_paragallinarum_serovar_C_hemagglutinating_antigen_ DB - PRIME DP - Unbound Medicine ER -