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Preparation of a magnetic molecularly imprinted polymer with pseudo template for rapid simultaneous determination of cyromazine and melamine in bio-matrix samples.
Anal Bioanal Chem. 2012 Sep; 404(5):1555-64.AB

Abstract

A magnetic molecularly imprinted polymer (M-MIP) for cyromazine and melamine was prepared by simple suspension polymerization using a pseudo template, 2-(4,6-diamino-1,3,5-triazin-2-ylamino)ethanethiol disulfide. The M-MIP was characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, and vibrating sample magnetometry. Molecular recognition properties and binding capability to cyromazine and melamine were evaluated by adsorption testing, which showed the M-MIP had better affinity and selectivity than the magnetic non-imprinted polymer (M-NIP) for cyromazine and melamine. A method based on molecularly imprinted solid-phase extraction assisted by magnetic separation was developed for extraction of cyromazine and melamine from bio-matrix samples. Various conditions, for example desorption conditions, amount of M-MIP, extraction time, and sample pH were optimized. High-performance liquid chromatography with UV detection was used to determine cyromazine and melamine after extraction. The proposed method was successfully applied to determination of cyromazine and melamine in egg and milk samples. Recovery of standard spiked cyromazine and melamine from these samples was between 71.86 and 80.57%, with intraday and interday relative standard deviation ranging from 3.45 to 6.39% and from 3.95 to 7.84%, respectively. The results indicate that the pseudo template M-MIP can be used for preconcentration, purification, and analysis of cyromazine and melamine in bio-matrix samples.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Wang X
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
Fang Q
No affiliation info available
Liu S
No affiliation info available
Chen L
No affiliation info available

MeSH

AdsorptionAnimalsChromatography, High Pressure LiquidEggsInsecticidesMagnetsMilkMolecular ImprintingPolymerizationPolymersSensitivity and SpecificitySolid Phase ExtractionTriazines

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22820903

Citation

Wang, Xianhua, et al. "Preparation of a Magnetic Molecularly Imprinted Polymer With Pseudo Template for Rapid Simultaneous Determination of Cyromazine and Melamine in Bio-matrix Samples." Analytical and Bioanalytical Chemistry, vol. 404, no. 5, 2012, pp. 1555-64.
Wang X, Fang Q, Liu S, et al. Preparation of a magnetic molecularly imprinted polymer with pseudo template for rapid simultaneous determination of cyromazine and melamine in bio-matrix samples. Anal Bioanal Chem. 2012;404(5):1555-64.
Wang, X., Fang, Q., Liu, S., & Chen, L. (2012). Preparation of a magnetic molecularly imprinted polymer with pseudo template for rapid simultaneous determination of cyromazine and melamine in bio-matrix samples. Analytical and Bioanalytical Chemistry, 404(5), 1555-64. https://doi.org/10.1007/s00216-012-6200-7
Wang X, et al. Preparation of a Magnetic Molecularly Imprinted Polymer With Pseudo Template for Rapid Simultaneous Determination of Cyromazine and Melamine in Bio-matrix Samples. Anal Bioanal Chem. 2012;404(5):1555-64. PubMed PMID: 22820903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation of a magnetic molecularly imprinted polymer with pseudo template for rapid simultaneous determination of cyromazine and melamine in bio-matrix samples. AU - Wang,Xianhua, AU - Fang,Qiuxue, AU - Liu,Shipeng, AU - Chen,Lei, Y1 - 2012/07/21/ PY - 2012/02/24/received PY - 2012/06/14/accepted PY - 2012/06/13/revised PY - 2012/7/24/entrez PY - 2012/7/24/pubmed PY - 2013/3/23/medline SP - 1555 EP - 64 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 404 IS - 5 N2 - A magnetic molecularly imprinted polymer (M-MIP) for cyromazine and melamine was prepared by simple suspension polymerization using a pseudo template, 2-(4,6-diamino-1,3,5-triazin-2-ylamino)ethanethiol disulfide. The M-MIP was characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, and vibrating sample magnetometry. Molecular recognition properties and binding capability to cyromazine and melamine were evaluated by adsorption testing, which showed the M-MIP had better affinity and selectivity than the magnetic non-imprinted polymer (M-NIP) for cyromazine and melamine. A method based on molecularly imprinted solid-phase extraction assisted by magnetic separation was developed for extraction of cyromazine and melamine from bio-matrix samples. Various conditions, for example desorption conditions, amount of M-MIP, extraction time, and sample pH were optimized. High-performance liquid chromatography with UV detection was used to determine cyromazine and melamine after extraction. The proposed method was successfully applied to determination of cyromazine and melamine in egg and milk samples. Recovery of standard spiked cyromazine and melamine from these samples was between 71.86 and 80.57%, with intraday and interday relative standard deviation ranging from 3.45 to 6.39% and from 3.95 to 7.84%, respectively. The results indicate that the pseudo template M-MIP can be used for preconcentration, purification, and analysis of cyromazine and melamine in bio-matrix samples. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/22820903/Preparation_of_a_magnetic_molecularly_imprinted_polymer_with_pseudo_template_for_rapid_simultaneous_determination_of_cyromazine_and_melamine_in_bio_matrix_samples_ L2 - https://dx.doi.org/10.1007/s00216-012-6200-7 DB - PRIME DP - Unbound Medicine ER -
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