Tags

Type your tag names separated by a space and hit enter

Talin couples the actomyosin cortex to the plasma membrane during rear retraction and cytokinesis.
Proc Natl Acad Sci U S A 2012; 109(32):12992-7PN

Abstract

Contraction of the cortical actin cytoskeleton underlies both rear retraction in directed cell migration and cytokinesis. Here, we show that talin, a central component of focal adhesions, has a major role in these processes. We found that Dictyostelium talin A colocalized with myosin II in the rear of migrating cells and the cleavage furrow. During directed cell migration, talin A-null cells displayed a long thin tail devoid of actin filaments, whereas additional depletion of SibA, a transmembrane adhesion molecule that binds to talin A, reverted this phenotype, suggesting a requirement of the link between actomyosin and SibA by talin A for rear retraction. Disruptions of talin A also resulted in detachment of the actomyosin contractile ring from the cell membrane and concomitant regression of the cleavage furrow under certain conditions. The C-terminal actin-binding domain (ABD) of talin A exhibited a localization pattern identical to that of full-length talin A. The N-terminal FERM domain was found to bind phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] and phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P3] in vitro. In vivo, however, PtdIns(4,5)P2, which is known to activate talin, is believed to be enriched in the rear of migrating cells and the cleavage furrow in Dictyostelium. From these results, we propose that talin A activated by PtdIns(4,5)P2 in the cell posterior or cleavage furrow links actomyosin cytoskeleton to adhesion molecules or other membrane proteins, and that the force is transmitted through these links to retract the tail during cell migration or to cause efficient ingression of the equator during cytokinesis.

Authors+Show Affiliations

Electron Microscope Laboratory, Center for Developmental Biology, RIKEN, Chuo-ku, Kobe 650-0047, Japan. m-tsujioka@fbs.osaka-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22826231

Citation

Tsujioka, Masatsune, et al. "Talin Couples the Actomyosin Cortex to the Plasma Membrane During Rear Retraction and Cytokinesis." Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 32, 2012, pp. 12992-7.
Tsujioka M, Yumura S, Inouye K, et al. Talin couples the actomyosin cortex to the plasma membrane during rear retraction and cytokinesis. Proc Natl Acad Sci USA. 2012;109(32):12992-7.
Tsujioka, M., Yumura, S., Inouye, K., Patel, H., Ueda, M., & Yonemura, S. (2012). Talin couples the actomyosin cortex to the plasma membrane during rear retraction and cytokinesis. Proceedings of the National Academy of Sciences of the United States of America, 109(32), pp. 12992-7. doi:10.1073/pnas.1208296109.
Tsujioka M, et al. Talin Couples the Actomyosin Cortex to the Plasma Membrane During Rear Retraction and Cytokinesis. Proc Natl Acad Sci USA. 2012 Aug 7;109(32):12992-7. PubMed PMID: 22826231.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Talin couples the actomyosin cortex to the plasma membrane during rear retraction and cytokinesis. AU - Tsujioka,Masatsune, AU - Yumura,Shigehiko, AU - Inouye,Kei, AU - Patel,Hitesh, AU - Ueda,Masahiro, AU - Yonemura,Shigenobu, Y1 - 2012/07/23/ PY - 2012/7/25/entrez PY - 2012/7/25/pubmed PY - 2012/10/16/medline SP - 12992 EP - 7 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 109 IS - 32 N2 - Contraction of the cortical actin cytoskeleton underlies both rear retraction in directed cell migration and cytokinesis. Here, we show that talin, a central component of focal adhesions, has a major role in these processes. We found that Dictyostelium talin A colocalized with myosin II in the rear of migrating cells and the cleavage furrow. During directed cell migration, talin A-null cells displayed a long thin tail devoid of actin filaments, whereas additional depletion of SibA, a transmembrane adhesion molecule that binds to talin A, reverted this phenotype, suggesting a requirement of the link between actomyosin and SibA by talin A for rear retraction. Disruptions of talin A also resulted in detachment of the actomyosin contractile ring from the cell membrane and concomitant regression of the cleavage furrow under certain conditions. The C-terminal actin-binding domain (ABD) of talin A exhibited a localization pattern identical to that of full-length talin A. The N-terminal FERM domain was found to bind phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] and phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P3] in vitro. In vivo, however, PtdIns(4,5)P2, which is known to activate talin, is believed to be enriched in the rear of migrating cells and the cleavage furrow in Dictyostelium. From these results, we propose that talin A activated by PtdIns(4,5)P2 in the cell posterior or cleavage furrow links actomyosin cytoskeleton to adhesion molecules or other membrane proteins, and that the force is transmitted through these links to retract the tail during cell migration or to cause efficient ingression of the equator during cytokinesis. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/22826231/Talin_couples_the_actomyosin_cortex_to_the_plasma_membrane_during_rear_retraction_and_cytokinesis_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=22826231 DB - PRIME DP - Unbound Medicine ER -