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How malaria parasites reduce the deformability of infected red blood cells.
Biophys J 2012; 103(1):1-10BJ

Abstract

The pathogenesis of malaria is largely due to stiffening of the infected red blood cells (RBCs). Contemporary understanding ascribes the loss of RBC deformability to a 10-fold increase in membrane stiffness caused by extra cross-linking in the spectrin network. Local measurements by micropipette aspiration, however, have reported only an increase of ∼3-fold in the shear modulus. We believe the discrepancy stems from the rigid parasite particles inside infected cells, and have carried out numerical simulations to demonstrate this mechanism. The cell membrane is represented by a set of discrete particles connected by linearly elastic springs. The cytosol is modeled as a homogeneous Newtonian fluid, and discretized by particles as in standard smoothed particle hydrodynamics. The malaria parasite is modeled as an aggregate of particles constrained to rigid-body motion. We simulate RBC stretching tests by optical tweezers in three dimensions. The results demonstrate that the presence of a sizeable parasite greatly reduces the ability of RBCs to deform under stretching. With the solid inclusion, the observed loss of deformability can be predicted quantitatively using the local membrane elasticity measured by micropipettes.

Authors+Show Affiliations

Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, British Columbia, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22828326

Citation

Hosseini, S Majid, and James J. Feng. "How Malaria Parasites Reduce the Deformability of Infected Red Blood Cells." Biophysical Journal, vol. 103, no. 1, 2012, pp. 1-10.
Hosseini SM, Feng JJ. How malaria parasites reduce the deformability of infected red blood cells. Biophys J. 2012;103(1):1-10.
Hosseini, S. M., & Feng, J. J. (2012). How malaria parasites reduce the deformability of infected red blood cells. Biophysical Journal, 103(1), pp. 1-10. doi:10.1016/j.bpj.2012.05.026.
Hosseini SM, Feng JJ. How Malaria Parasites Reduce the Deformability of Infected Red Blood Cells. Biophys J. 2012 Jul 3;103(1):1-10. PubMed PMID: 22828326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - How malaria parasites reduce the deformability of infected red blood cells. AU - Hosseini,S Majid, AU - Feng,James J, PY - 2012/03/01/received PY - 2012/04/23/revised PY - 2012/05/15/accepted PY - 2012/7/26/entrez PY - 2012/7/26/pubmed PY - 2012/12/12/medline SP - 1 EP - 10 JF - Biophysical journal JO - Biophys. J. VL - 103 IS - 1 N2 - The pathogenesis of malaria is largely due to stiffening of the infected red blood cells (RBCs). Contemporary understanding ascribes the loss of RBC deformability to a 10-fold increase in membrane stiffness caused by extra cross-linking in the spectrin network. Local measurements by micropipette aspiration, however, have reported only an increase of ∼3-fold in the shear modulus. We believe the discrepancy stems from the rigid parasite particles inside infected cells, and have carried out numerical simulations to demonstrate this mechanism. The cell membrane is represented by a set of discrete particles connected by linearly elastic springs. The cytosol is modeled as a homogeneous Newtonian fluid, and discretized by particles as in standard smoothed particle hydrodynamics. The malaria parasite is modeled as an aggregate of particles constrained to rigid-body motion. We simulate RBC stretching tests by optical tweezers in three dimensions. The results demonstrate that the presence of a sizeable parasite greatly reduces the ability of RBCs to deform under stretching. With the solid inclusion, the observed loss of deformability can be predicted quantitatively using the local membrane elasticity measured by micropipettes. SN - 1542-0086 UR - https://www.unboundmedicine.com/medline/citation/22828326/How_malaria_parasites_reduce_the_deformability_of_infected_red_blood_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-3495(12)00579-6 DB - PRIME DP - Unbound Medicine ER -