Tags

Type your tag names separated by a space and hit enter

Pathogenicity and transmissibility of North American triple reassortant swine influenza A viruses in ferrets.
PLoS Pathog. 2012; 8(7):e1002791.PP

Abstract

North American triple reassortant swine (TRS) influenza A viruses have caused sporadic human infections since 2005, but human-to-human transmission has not been documented. These viruses have six gene segments (PB2, PB1, PA, HA, NP, and NS) closely related to those of the 2009 H1N1 pandemic viruses. Therefore, understanding of these viruses' pathogenicity and transmissibility may help to identify determinants of virulence of the 2009 H1N1 pandemic viruses and to elucidate potential human health threats posed by the TRS viruses. Here we evaluated in a ferret model the pathogenicity and transmissibility of three groups of North American TRS viruses containing swine-like and/or human-like HA and NA gene segments. The study was designed only to detect informative and significant patterns in the transmissibility and pathogenicity of these three groups of viruses. We observed that irrespective of their HA and NA lineages, the TRS viruses were moderately pathogenic in ferrets and grew efficiently in both the upper and lower respiratory tracts. All North American TRS viruses studied were transmitted between ferrets via direct contact. However, their transmissibility by respiratory droplets was related to their HA and NA lineages: TRS viruses with human-like HA and NA were transmitted most efficiently, those with swine-like HA and NA were transmitted minimally or not transmitted, and those with swine-like HA and human-like NA (N2) showed intermediate transmissibility. We conclude that the lineages of HA and NA may play a crucial role in the respiratory droplet transmissibility of these viruses. These findings have important implications for pandemic planning and warrant confirmation.

Authors+Show Affiliations

Division of Virology, Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, United States of America.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22829764

Citation

Barman, Subrata, et al. "Pathogenicity and Transmissibility of North American Triple Reassortant Swine Influenza a Viruses in Ferrets." PLoS Pathogens, vol. 8, no. 7, 2012, pp. e1002791.
Barman S, Krylov PS, Fabrizio TP, et al. Pathogenicity and transmissibility of North American triple reassortant swine influenza A viruses in ferrets. PLoS Pathog. 2012;8(7):e1002791.
Barman, S., Krylov, P. S., Fabrizio, T. P., Franks, J., Turner, J. C., Seiler, P., Wang, D., Rehg, J. E., Erickson, G. A., Gramer, M., Webster, R. G., & Webby, R. J. (2012). Pathogenicity and transmissibility of North American triple reassortant swine influenza A viruses in ferrets. PLoS Pathogens, 8(7), e1002791. https://doi.org/10.1371/journal.ppat.1002791
Barman S, et al. Pathogenicity and Transmissibility of North American Triple Reassortant Swine Influenza a Viruses in Ferrets. PLoS Pathog. 2012;8(7):e1002791. PubMed PMID: 22829764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pathogenicity and transmissibility of North American triple reassortant swine influenza A viruses in ferrets. AU - Barman,Subrata, AU - Krylov,Petr S, AU - Fabrizio,Thomas P, AU - Franks,John, AU - Turner,Jasmine C, AU - Seiler,Patrick, AU - Wang,David, AU - Rehg,Jerold E, AU - Erickson,Gene A, AU - Gramer,Marie, AU - Webster,Robert G, AU - Webby,Richard J, Y1 - 2012/07/19/ PY - 2011/12/08/received PY - 2012/05/22/accepted PY - 2012/7/26/entrez PY - 2012/7/26/pubmed PY - 2013/1/30/medline SP - e1002791 EP - e1002791 JF - PLoS pathogens JO - PLoS Pathog. VL - 8 IS - 7 N2 - North American triple reassortant swine (TRS) influenza A viruses have caused sporadic human infections since 2005, but human-to-human transmission has not been documented. These viruses have six gene segments (PB2, PB1, PA, HA, NP, and NS) closely related to those of the 2009 H1N1 pandemic viruses. Therefore, understanding of these viruses' pathogenicity and transmissibility may help to identify determinants of virulence of the 2009 H1N1 pandemic viruses and to elucidate potential human health threats posed by the TRS viruses. Here we evaluated in a ferret model the pathogenicity and transmissibility of three groups of North American TRS viruses containing swine-like and/or human-like HA and NA gene segments. The study was designed only to detect informative and significant patterns in the transmissibility and pathogenicity of these three groups of viruses. We observed that irrespective of their HA and NA lineages, the TRS viruses were moderately pathogenic in ferrets and grew efficiently in both the upper and lower respiratory tracts. All North American TRS viruses studied were transmitted between ferrets via direct contact. However, their transmissibility by respiratory droplets was related to their HA and NA lineages: TRS viruses with human-like HA and NA were transmitted most efficiently, those with swine-like HA and NA were transmitted minimally or not transmitted, and those with swine-like HA and human-like NA (N2) showed intermediate transmissibility. We conclude that the lineages of HA and NA may play a crucial role in the respiratory droplet transmissibility of these viruses. These findings have important implications for pandemic planning and warrant confirmation. SN - 1553-7374 UR - https://www.unboundmedicine.com/medline/citation/22829764/Pathogenicity_and_transmissibility_of_North_American_triple_reassortant_swine_influenza_A_viruses_in_ferrets_ L2 - http://dx.plos.org/10.1371/journal.ppat.1002791 DB - PRIME DP - Unbound Medicine ER -