Tags

Type your tag names separated by a space and hit enter

Metabolic profiling of the methylerythritol phosphate pathway reveals the source of post-illumination isoprene burst from leaves.
Plant Cell Environ. 2013 Feb; 36(2):429-37.PC

Abstract

The methylerythritol phosphate (MEP) pathway in plants produces the prenyl precursors for all plastidic isoprenoids, including carotenoids and quinones. The MEP pathway is also responsible for synthesis of approximately 600 Tg of isoprene per year, the largest non-methane hydrocarbon flux into the atmosphere. There have been few studies of the regulation of the MEP pathway in plants under physiological conditions. In this study, we combined gas exchange techniques and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) and measured the profile of MEP pathway metabolites under different conditions. We report that in the MEP pathway, metabolites immediately preceding steps requiring reducing power were in high concentration. Inhibition of the MEP pathway by fosmidomycin caused deoxyxylulose phosphate accumulation in leaves as expected. Evidence is presented that accumulation of MEP pathway intermediates, primarily methylerythritol cyclodiphosphate, is responsible for the post-illumination isoprene burst phenomenon. Pools of intermediate metabolites stayed at approximately the same level 10 min after light was turned off, but declined eventually under prolonged darkness. In contrast, a strong inhibition of the second-to-last step of the MEP pathway caused suppression of isoprene emission in pure N(2). Our study suggests that reducing equivalents may be a key regulator of the MEP pathway and therefore isoprene emission from leaves.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Li Z
Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
Sharkey TD
No affiliation info available

MeSH

AcclimatizationButadienesChromatography, High Pressure LiquidDarknessErythritolFosfomycinHemiterpenesLightMass SpectrometryMetabolic Networks and PathwaysMetabolomeNitrogenPentanesPlant ExtractsPlant LeavesPopulusReference StandardsTime Factors

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22831282

Citation

Li, Ziru, and Thomas D. Sharkey. "Metabolic Profiling of the Methylerythritol Phosphate Pathway Reveals the Source of Post-illumination Isoprene Burst From Leaves." Plant, Cell & Environment, vol. 36, no. 2, 2013, pp. 429-37.
Li Z, Sharkey TD. Metabolic profiling of the methylerythritol phosphate pathway reveals the source of post-illumination isoprene burst from leaves. Plant Cell Environ. 2013;36(2):429-37.
Li, Z., & Sharkey, T. D. (2013). Metabolic profiling of the methylerythritol phosphate pathway reveals the source of post-illumination isoprene burst from leaves. Plant, Cell & Environment, 36(2), 429-37. https://doi.org/10.1111/j.1365-3040.2012.02584.x
Li Z, Sharkey TD. Metabolic Profiling of the Methylerythritol Phosphate Pathway Reveals the Source of Post-illumination Isoprene Burst From Leaves. Plant Cell Environ. 2013;36(2):429-37. PubMed PMID: 22831282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic profiling of the methylerythritol phosphate pathway reveals the source of post-illumination isoprene burst from leaves. AU - Li,Ziru, AU - Sharkey,Thomas D, Y1 - 2012/08/14/ PY - 2012/7/27/entrez PY - 2012/7/27/pubmed PY - 2013/6/12/medline SP - 429 EP - 37 JF - Plant, cell & environment JO - Plant Cell Environ VL - 36 IS - 2 N2 - The methylerythritol phosphate (MEP) pathway in plants produces the prenyl precursors for all plastidic isoprenoids, including carotenoids and quinones. The MEP pathway is also responsible for synthesis of approximately 600 Tg of isoprene per year, the largest non-methane hydrocarbon flux into the atmosphere. There have been few studies of the regulation of the MEP pathway in plants under physiological conditions. In this study, we combined gas exchange techniques and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) and measured the profile of MEP pathway metabolites under different conditions. We report that in the MEP pathway, metabolites immediately preceding steps requiring reducing power were in high concentration. Inhibition of the MEP pathway by fosmidomycin caused deoxyxylulose phosphate accumulation in leaves as expected. Evidence is presented that accumulation of MEP pathway intermediates, primarily methylerythritol cyclodiphosphate, is responsible for the post-illumination isoprene burst phenomenon. Pools of intermediate metabolites stayed at approximately the same level 10 min after light was turned off, but declined eventually under prolonged darkness. In contrast, a strong inhibition of the second-to-last step of the MEP pathway caused suppression of isoprene emission in pure N(2). Our study suggests that reducing equivalents may be a key regulator of the MEP pathway and therefore isoprene emission from leaves. SN - 1365-3040 UR - https://www.unboundmedicine.com/medline/citation/22831282/Metabolic_profiling_of_the_methylerythritol_phosphate_pathway_reveals_the_source_of_post_illumination_isoprene_burst_from_leaves_ L2 - https://doi.org/10.1111/j.1365-3040.2012.02584.x DB - PRIME DP - Unbound Medicine ER -