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Effects of LPS on P2X3 receptors of trigeminal sensory neurons and macrophages from mice expressing the R192Q Cacna1a gene mutation of familial hemiplegic migraine-1.
Purinergic Signal 2013; 9(1):7-13PS

Abstract

A knockin (KI) mouse model with the R192Q missense mutation in the Cacna1a gene commonly detected in familial hemiplegic migraine was used to study whether trigeminal ganglia showed a basal inflammatory profile that could be further enhanced by the lipopolysaccharide (LPS) toxin. Adenosine-5'-triphosphate (ATP)-gated purinergic ionotropic receptor 3 (P2X3) currents expressed by the large majority of trigeminal sensory neurons were taken as functional readout. Cultured R192Q KI trigeminal ganglia showed higher number of active macrophages, basal release of tumor necrosis factor alpha (TNFα), and larger P2X3 receptor currents with respect to wild type (WT) cells. After 5 h application of LPS in vitro, both WT and R192Q KI cultures demonstrated significant increase in macrophage activation, very large rise in TNFα mRNA content, and ambient protein levels together with fall in TNFα precursor, suggesting potent release of this inflammatory mediator. Notwithstanding the unchanged expression of P2X3 receptor protein in WT or R192Q KI cultures, LPS evoked a large rise in WT neuronal currents that recovered faster from desensitization. Basal R192Q KI currents were larger than WT ones and could not be further augmented by LPS. These data suggest that KI cultures had a basal neuroinflammatory profile that might facilitate the release of endogenous mediators (including ATP) to activate constitutively hyperfunctional P2X3 receptors and amplify nociceptive signaling by trigeminal sensory neurons.

Authors+Show Affiliations

Department of Neuroscience and Italian Institute of Technology Unit, International School for Advanced Studies (SISSA), Via Bonomea 265, 34136, Trieste, Italy. alessia.franceschini@sissa.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22836594

Citation

Franceschini, Alessia, et al. "Effects of LPS On P2X3 Receptors of Trigeminal Sensory Neurons and Macrophages From Mice Expressing the R192Q Cacna1a Gene Mutation of Familial Hemiplegic Migraine-1." Purinergic Signalling, vol. 9, no. 1, 2013, pp. 7-13.
Franceschini A, Hullugundi SK, van den Maagdenberg AM, et al. Effects of LPS on P2X3 receptors of trigeminal sensory neurons and macrophages from mice expressing the R192Q Cacna1a gene mutation of familial hemiplegic migraine-1. Purinergic Signal. 2013;9(1):7-13.
Franceschini, A., Hullugundi, S. K., van den Maagdenberg, A. M., Nistri, A., & Fabbretti, E. (2013). Effects of LPS on P2X3 receptors of trigeminal sensory neurons and macrophages from mice expressing the R192Q Cacna1a gene mutation of familial hemiplegic migraine-1. Purinergic Signalling, 9(1), pp. 7-13. doi:10.1007/s11302-012-9328-1.
Franceschini A, et al. Effects of LPS On P2X3 Receptors of Trigeminal Sensory Neurons and Macrophages From Mice Expressing the R192Q Cacna1a Gene Mutation of Familial Hemiplegic Migraine-1. Purinergic Signal. 2013;9(1):7-13. PubMed PMID: 22836594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of LPS on P2X3 receptors of trigeminal sensory neurons and macrophages from mice expressing the R192Q Cacna1a gene mutation of familial hemiplegic migraine-1. AU - Franceschini,Alessia, AU - Hullugundi,Swathi K, AU - van den Maagdenberg,Arn M J M, AU - Nistri,Andrea, AU - Fabbretti,Elsa, Y1 - 2012/07/27/ PY - 2012/04/19/received PY - 2012/07/06/accepted PY - 2012/7/28/entrez PY - 2012/7/28/pubmed PY - 2013/7/28/medline SP - 7 EP - 13 JF - Purinergic signalling JO - Purinergic Signal. VL - 9 IS - 1 N2 - A knockin (KI) mouse model with the R192Q missense mutation in the Cacna1a gene commonly detected in familial hemiplegic migraine was used to study whether trigeminal ganglia showed a basal inflammatory profile that could be further enhanced by the lipopolysaccharide (LPS) toxin. Adenosine-5'-triphosphate (ATP)-gated purinergic ionotropic receptor 3 (P2X3) currents expressed by the large majority of trigeminal sensory neurons were taken as functional readout. Cultured R192Q KI trigeminal ganglia showed higher number of active macrophages, basal release of tumor necrosis factor alpha (TNFα), and larger P2X3 receptor currents with respect to wild type (WT) cells. After 5 h application of LPS in vitro, both WT and R192Q KI cultures demonstrated significant increase in macrophage activation, very large rise in TNFα mRNA content, and ambient protein levels together with fall in TNFα precursor, suggesting potent release of this inflammatory mediator. Notwithstanding the unchanged expression of P2X3 receptor protein in WT or R192Q KI cultures, LPS evoked a large rise in WT neuronal currents that recovered faster from desensitization. Basal R192Q KI currents were larger than WT ones and could not be further augmented by LPS. These data suggest that KI cultures had a basal neuroinflammatory profile that might facilitate the release of endogenous mediators (including ATP) to activate constitutively hyperfunctional P2X3 receptors and amplify nociceptive signaling by trigeminal sensory neurons. SN - 1573-9546 UR - https://www.unboundmedicine.com/medline/citation/22836594/Effects_of_LPS_on_P2X3_receptors_of_trigeminal_sensory_neurons_and_macrophages_from_mice_expressing_the_R192Q_Cacna1a_gene_mutation_of_familial_hemiplegic_migraine_1_ L2 - https://dx.doi.org/10.1007/s11302-012-9328-1 DB - PRIME DP - Unbound Medicine ER -