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Antidiabetic and antioxidant activities of Toddalia asiatica (L.) Lam. leaves in streptozotocin induced diabetic rats.
J Ethnopharmacol. 2012 Sep 28; 143(2):515-23.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The leaves of Toddalia asiatica (L.) Lam. have been utilized traditionally for the cure of diabetes.

AIM OF THE STUDY

The present study was aimed to assess the antidiabetic and antioxidant effects of T. asiatica leaves in Streptozotocin (STZ) induced diabetic rats.

MATERIALS AND METHODS

The phytochemical screening, total phenolic content, HPLC analysis, acute toxicity study and oral glucose tolerance test were carried out. Glucose lowering effect of the hexane, ethyl acetate and methanol extracts of T. asiatica leaves was studied in STZ-induced diabetic rats. The antidiabetic and antioxidant activities were studied for the ethyl acetate extract. The effects of extracts on blood glucose, body weight, plasma insulin, total protein, liver glycogen, plasma enzymes (SGOT, SGPT and ALP) and activities of SOD, CAT and GPx were analyzed.

RESULTS

T. asiatica leaves ethyl acetate extract (TALEe) showed highly significant blood glucose lowering effect. Phytochemical evaluation of TALEe showed the presence of alkaloids, terpenoids, cumarins, flavonoids and phenolic compounds. The total phenolic content of TALEe was 126 mg of gallic acid equivalents/g extract. HPLC analysis revealed the presence of flindersine and ulopterol. Acute toxicity study of TALEe revealed no death or toxicity. The oral glucose tolerance test showed lowered area under curve (AUC(glucose)) values in TALEe treated rats. After treatment with TALEe (250 and 500 mg/kg) for 28 days there was a significant decrease in blood glucose, plasma enzymes (SGOT, SGPT and ALP) and significant increase in body weight, total protein, serum insulin and liver glycogen levels in treated diabetic rats. The activities of antioxidant enzymes SOD, CAT and GPx were reversed to near normal in treated diabetic rats. Histopathology of pancreas in TALEe treated groups showed regeneration of β-cells.

CONCLUSION

The results of the experiments showed that TALEe exerted significant antidiabetic and antioxidant effects in STZ-induced diabetic rats justifying its traditional use.

Authors+Show Affiliations

Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600034, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22842651

Citation

Stephen Irudayaraj, Santiagu, et al. "Antidiabetic and Antioxidant Activities of Toddalia Asiatica (L.) Lam. Leaves in Streptozotocin Induced Diabetic Rats." Journal of Ethnopharmacology, vol. 143, no. 2, 2012, pp. 515-23.
Stephen Irudayaraj S, Sunil C, Duraipandiyan V, et al. Antidiabetic and antioxidant activities of Toddalia asiatica (L.) Lam. leaves in streptozotocin induced diabetic rats. J Ethnopharmacol. 2012;143(2):515-23.
Stephen Irudayaraj, S., Sunil, C., Duraipandiyan, V., & Ignacimuthu, S. (2012). Antidiabetic and antioxidant activities of Toddalia asiatica (L.) Lam. leaves in streptozotocin induced diabetic rats. Journal of Ethnopharmacology, 143(2), 515-23. https://doi.org/10.1016/j.jep.2012.07.006
Stephen Irudayaraj S, et al. Antidiabetic and Antioxidant Activities of Toddalia Asiatica (L.) Lam. Leaves in Streptozotocin Induced Diabetic Rats. J Ethnopharmacol. 2012 Sep 28;143(2):515-23. PubMed PMID: 22842651.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antidiabetic and antioxidant activities of Toddalia asiatica (L.) Lam. leaves in streptozotocin induced diabetic rats. AU - Stephen Irudayaraj,Santiagu, AU - Sunil,Christudas, AU - Duraipandiyan,Veeramuthu, AU - Ignacimuthu,Savarimuthu, Y1 - 2012/07/27/ PY - 2012/02/24/received PY - 2012/06/26/revised PY - 2012/07/09/accepted PY - 2012/7/31/entrez PY - 2012/7/31/pubmed PY - 2013/1/30/medline SP - 515 EP - 23 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 143 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The leaves of Toddalia asiatica (L.) Lam. have been utilized traditionally for the cure of diabetes. AIM OF THE STUDY: The present study was aimed to assess the antidiabetic and antioxidant effects of T. asiatica leaves in Streptozotocin (STZ) induced diabetic rats. MATERIALS AND METHODS: The phytochemical screening, total phenolic content, HPLC analysis, acute toxicity study and oral glucose tolerance test were carried out. Glucose lowering effect of the hexane, ethyl acetate and methanol extracts of T. asiatica leaves was studied in STZ-induced diabetic rats. The antidiabetic and antioxidant activities were studied for the ethyl acetate extract. The effects of extracts on blood glucose, body weight, plasma insulin, total protein, liver glycogen, plasma enzymes (SGOT, SGPT and ALP) and activities of SOD, CAT and GPx were analyzed. RESULTS: T. asiatica leaves ethyl acetate extract (TALEe) showed highly significant blood glucose lowering effect. Phytochemical evaluation of TALEe showed the presence of alkaloids, terpenoids, cumarins, flavonoids and phenolic compounds. The total phenolic content of TALEe was 126 mg of gallic acid equivalents/g extract. HPLC analysis revealed the presence of flindersine and ulopterol. Acute toxicity study of TALEe revealed no death or toxicity. The oral glucose tolerance test showed lowered area under curve (AUC(glucose)) values in TALEe treated rats. After treatment with TALEe (250 and 500 mg/kg) for 28 days there was a significant decrease in blood glucose, plasma enzymes (SGOT, SGPT and ALP) and significant increase in body weight, total protein, serum insulin and liver glycogen levels in treated diabetic rats. The activities of antioxidant enzymes SOD, CAT and GPx were reversed to near normal in treated diabetic rats. Histopathology of pancreas in TALEe treated groups showed regeneration of β-cells. CONCLUSION: The results of the experiments showed that TALEe exerted significant antidiabetic and antioxidant effects in STZ-induced diabetic rats justifying its traditional use. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22842651/Antidiabetic_and_antioxidant_activities_of_Toddalia_asiatica__L___Lam__leaves_in_streptozotocin_induced_diabetic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00475-8 DB - PRIME DP - Unbound Medicine ER -