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Enhancing the lateral-flow immunoassay for detection of proteins using an aqueous two-phase micellar system.
Anal Bioanal Chem. 2012 Oct; 404(6-7):2057-66.AB

Abstract

The lateral-flow (immuno)assay (LFA) has been widely investigated for the detection of molecular, macromolecular, and particle targets at the point-of-need due to its ease of use, rapid processing, and minimal power and laboratory equipment requirements. However, for some analytes, such as certain proteins, the detection limit of LFA is inferior to lab-based assays, such as the enzyme-linked immunosorbent assay, and needs to be improved. One solution for improving the detection limit of LFA is to concentrate the target protein in a solution prior to the detection step. In this study, a novel approach was used in the context of an aqueous two-phase micellar system comprised of the nonionic surfactant Triton X-114 to concentrate a model protein, namely transferrin, prior to LFA. Proteins have been shown to partition, or distribute, fairly evenly between the two phases of an aqueous two-phase system, which in turn results in their limited concentration in one of the two phases. Therefore, larger colloidal gold particles decorated with antibodies for transferrin were used in the concentration step to bind to transferrin and aid its partitioning into the top, micelle-poor phase. By manipulating the volume ratio of the two coexisting micellar phases and combining the concentration step with LFA, the transferrin detection limit of LFA was improved by tenfold from 0.5 to 0.05 μg/mL in a predictive manner. In addition to enhancing the sensitivity of LFA, this universal concentration method could also be used to improve other detection assays.

Authors+Show Affiliations

Department of Bioengineering, University of California, Los Angeles, CA 90095-1600, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22847478

Citation

Mashayekhi, Foad, et al. "Enhancing the Lateral-flow Immunoassay for Detection of Proteins Using an Aqueous Two-phase Micellar System." Analytical and Bioanalytical Chemistry, vol. 404, no. 6-7, 2012, pp. 2057-66.
Mashayekhi F, Le AM, Nafisi PM, et al. Enhancing the lateral-flow immunoassay for detection of proteins using an aqueous two-phase micellar system. Anal Bioanal Chem. 2012;404(6-7):2057-66.
Mashayekhi, F., Le, A. M., Nafisi, P. M., Wu, B. M., & Kamei, D. T. (2012). Enhancing the lateral-flow immunoassay for detection of proteins using an aqueous two-phase micellar system. Analytical and Bioanalytical Chemistry, 404(6-7), 2057-66. https://doi.org/10.1007/s00216-012-6278-y
Mashayekhi F, et al. Enhancing the Lateral-flow Immunoassay for Detection of Proteins Using an Aqueous Two-phase Micellar System. Anal Bioanal Chem. 2012;404(6-7):2057-66. PubMed PMID: 22847478.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhancing the lateral-flow immunoassay for detection of proteins using an aqueous two-phase micellar system. AU - Mashayekhi,Foad, AU - Le,Alexander M, AU - Nafisi,Parsa M, AU - Wu,Benjamin M, AU - Kamei,Daniel T, Y1 - 2012/07/31/ PY - 2012/05/11/received PY - 2012/07/16/accepted PY - 2012/07/13/revised PY - 2012/8/1/entrez PY - 2012/8/1/pubmed PY - 2013/3/13/medline SP - 2057 EP - 66 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 404 IS - 6-7 N2 - The lateral-flow (immuno)assay (LFA) has been widely investigated for the detection of molecular, macromolecular, and particle targets at the point-of-need due to its ease of use, rapid processing, and minimal power and laboratory equipment requirements. However, for some analytes, such as certain proteins, the detection limit of LFA is inferior to lab-based assays, such as the enzyme-linked immunosorbent assay, and needs to be improved. One solution for improving the detection limit of LFA is to concentrate the target protein in a solution prior to the detection step. In this study, a novel approach was used in the context of an aqueous two-phase micellar system comprised of the nonionic surfactant Triton X-114 to concentrate a model protein, namely transferrin, prior to LFA. Proteins have been shown to partition, or distribute, fairly evenly between the two phases of an aqueous two-phase system, which in turn results in their limited concentration in one of the two phases. Therefore, larger colloidal gold particles decorated with antibodies for transferrin were used in the concentration step to bind to transferrin and aid its partitioning into the top, micelle-poor phase. By manipulating the volume ratio of the two coexisting micellar phases and combining the concentration step with LFA, the transferrin detection limit of LFA was improved by tenfold from 0.5 to 0.05 μg/mL in a predictive manner. In addition to enhancing the sensitivity of LFA, this universal concentration method could also be used to improve other detection assays. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/22847478/Enhancing_the_lateral_flow_immunoassay_for_detection_of_proteins_using_an_aqueous_two_phase_micellar_system_ L2 - https://dx.doi.org/10.1007/s00216-012-6278-y DB - PRIME DP - Unbound Medicine ER -