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Effects of alanyl-glutamine dipeptide on the expression of colon-inflammatory mediators during the recovery phase of colitis induced by dextran sulfate sodium.
Eur J Nutr. 2013 Apr; 52(3):1089-98.EJ

Abstract

PURPOSE

Glutamine (Gln) is a nutrient with immunomodulatory effects in metabolic stressed conditions. This study investigated the effects of Gln on colonic-inflammatory-mediator expression and mucosal repair in mice with dextran sulfate sodium (DSS)-induced colitis.

METHODS

C57BL/6 mice received distilled water containing 3 % DSS for 5 d to induce colitis. One of the DSS-treated groups was intraperitoneally injected with an alanyl (Ala)-Gln solution 3 days before (G-DSS) while the other group was administered Ala-Gln 3 days after colitis (DSS-G) was induced. The Ala-Gln solution provided 0.5 g Gln/kg/d. The saline-DSS group (S-DSS) received an identical amount of saline before and after colitis was induced to serve as a positive control.

RESULTS

The S-DSS group had a shorter colon length, higher plasma haptoglobin level, and more-severe colon inflammation. Also, the toll-like receptor (TLR)4 level, nuclear factor (NF)-κB activation, and inflammatory cytokine gene expression in the colon were higher than those of the normal control group. Gln administration either before or after colitis suppressed TLR4 protein levels, decreased plasma haptoglobin, and reduced colon inflammation. Histological inflammatory scores were also lowered. Compared to the post-colitis Gln group, preventive use of Gln had higher colon length, expressions of mucin 2, trefoil factor 3, and heat shock protein 72 genes were also upregulated in the colon.

CONCLUSIONS

These results suggest that Gln administered either before or after the colitis mitigated inflammation of colitis that was not observed in group without Gln injection. Prophylactic treatment with Gln had more-beneficial effects on reducing inflammatory markers and enhancing the recovery of mucosa in DSS-induced colitis.

Authors+Show Affiliations

School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu Hsin Street, Taipei, 110, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22847641

Citation

Hou, Yu-Chen, et al. "Effects of Alanyl-glutamine Dipeptide On the Expression of Colon-inflammatory Mediators During the Recovery Phase of Colitis Induced By Dextran Sulfate Sodium." European Journal of Nutrition, vol. 52, no. 3, 2013, pp. 1089-98.
Hou YC, Chu CC, Ko TL, et al. Effects of alanyl-glutamine dipeptide on the expression of colon-inflammatory mediators during the recovery phase of colitis induced by dextran sulfate sodium. Eur J Nutr. 2013;52(3):1089-98.
Hou, Y. C., Chu, C. C., Ko, T. L., Yeh, C. L., & Yeh, S. L. (2013). Effects of alanyl-glutamine dipeptide on the expression of colon-inflammatory mediators during the recovery phase of colitis induced by dextran sulfate sodium. European Journal of Nutrition, 52(3), 1089-98. https://doi.org/10.1007/s00394-012-0416-3
Hou YC, et al. Effects of Alanyl-glutamine Dipeptide On the Expression of Colon-inflammatory Mediators During the Recovery Phase of Colitis Induced By Dextran Sulfate Sodium. Eur J Nutr. 2013;52(3):1089-98. PubMed PMID: 22847641.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of alanyl-glutamine dipeptide on the expression of colon-inflammatory mediators during the recovery phase of colitis induced by dextran sulfate sodium. AU - Hou,Yu-Chen, AU - Chu,Chia-Chou, AU - Ko,Tsui-Ling, AU - Yeh,Chiu-Li, AU - Yeh,Sung-Ling, Y1 - 2012/07/31/ PY - 2012/03/20/received PY - 2012/06/27/accepted PY - 2012/8/1/entrez PY - 2012/8/1/pubmed PY - 2013/10/22/medline SP - 1089 EP - 98 JF - European journal of nutrition JO - Eur J Nutr VL - 52 IS - 3 N2 - PURPOSE: Glutamine (Gln) is a nutrient with immunomodulatory effects in metabolic stressed conditions. This study investigated the effects of Gln on colonic-inflammatory-mediator expression and mucosal repair in mice with dextran sulfate sodium (DSS)-induced colitis. METHODS: C57BL/6 mice received distilled water containing 3 % DSS for 5 d to induce colitis. One of the DSS-treated groups was intraperitoneally injected with an alanyl (Ala)-Gln solution 3 days before (G-DSS) while the other group was administered Ala-Gln 3 days after colitis (DSS-G) was induced. The Ala-Gln solution provided 0.5 g Gln/kg/d. The saline-DSS group (S-DSS) received an identical amount of saline before and after colitis was induced to serve as a positive control. RESULTS: The S-DSS group had a shorter colon length, higher plasma haptoglobin level, and more-severe colon inflammation. Also, the toll-like receptor (TLR)4 level, nuclear factor (NF)-κB activation, and inflammatory cytokine gene expression in the colon were higher than those of the normal control group. Gln administration either before or after colitis suppressed TLR4 protein levels, decreased plasma haptoglobin, and reduced colon inflammation. Histological inflammatory scores were also lowered. Compared to the post-colitis Gln group, preventive use of Gln had higher colon length, expressions of mucin 2, trefoil factor 3, and heat shock protein 72 genes were also upregulated in the colon. CONCLUSIONS: These results suggest that Gln administered either before or after the colitis mitigated inflammation of colitis that was not observed in group without Gln injection. Prophylactic treatment with Gln had more-beneficial effects on reducing inflammatory markers and enhancing the recovery of mucosa in DSS-induced colitis. SN - 1436-6215 UR - https://www.unboundmedicine.com/medline/citation/22847641/Effects_of_alanyl_glutamine_dipeptide_on_the_expression_of_colon_inflammatory_mediators_during_the_recovery_phase_of_colitis_induced_by_dextran_sulfate_sodium_ L2 - https://dx.doi.org/10.1007/s00394-012-0416-3 DB - PRIME DP - Unbound Medicine ER -