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Different transcriptional response to Xanthomonas citri subsp. citri between kumquat and sweet orange with contrasting canker tolerance.
PLoS One. 2012; 7(7):e41790.Plos

Abstract

Citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating biotic stresses affecting the citrus industry. Meiwa kumquat (Fortunella crassifolia) is canker-resistant, while Newhall navel orange (Citrus sinensis Osbeck) is canker-sensitive. To understand the molecular mechanisms underlying the differences in responses to Xcc, transcriptomic profiles of these two genotypes following Xcc attack were compared by using the Affymetrix citrus genome GeneChip. A total of 794 and 1324 differentially expressed genes (DEGs) were identified as canker-responsive genes in Meiwa and Newhall, respectively. Of these, 230 genes were expressed in common between both genotypes, while 564 and 1094 genes were only significantly expressed in either Meiwa or Newhall. Gene ontology (GO) annotation and Singular Enrichment Analysis (SEA) of the DEGs showed that genes related to the cell wall and polysaccharide metabolism were induced for basic defense in both Meiwa and Newhall, such as chitinase, glucanase and thaumatin-like protein. Moreover, apart from inducing basic defense, Meiwa showed specially upregulated expression of several genes involved in the response to biotic stimulus, defense response, and cation binding as comparing with Newhall. And in Newhall, abundant photosynthesis-related genes were significantly down-regulated, which may be in order to ensure the basic defense. This study revealed different molecular responses to canker disease in Meiwa and Newhall, affording insight into the response to canker and providing valuable information for the identification of potential genes for engineering canker tolerance in the future.

Authors+Show Affiliations

Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22848606

Citation

Fu, Xing-Zheng, et al. "Different Transcriptional Response to Xanthomonas Citri Subsp. Citri Between Kumquat and Sweet Orange With Contrasting Canker Tolerance." PloS One, vol. 7, no. 7, 2012, pp. e41790.
Fu XZ, Gong XQ, Zhang YX, et al. Different transcriptional response to Xanthomonas citri subsp. citri between kumquat and sweet orange with contrasting canker tolerance. PLoS One. 2012;7(7):e41790.
Fu, X. Z., Gong, X. Q., Zhang, Y. X., Wang, Y., & Liu, J. H. (2012). Different transcriptional response to Xanthomonas citri subsp. citri between kumquat and sweet orange with contrasting canker tolerance. PloS One, 7(7), e41790. https://doi.org/10.1371/journal.pone.0041790
Fu XZ, et al. Different Transcriptional Response to Xanthomonas Citri Subsp. Citri Between Kumquat and Sweet Orange With Contrasting Canker Tolerance. PLoS One. 2012;7(7):e41790. PubMed PMID: 22848606.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Different transcriptional response to Xanthomonas citri subsp. citri between kumquat and sweet orange with contrasting canker tolerance. AU - Fu,Xing-Zheng, AU - Gong,Xiao-Qing, AU - Zhang,Yue-Xin, AU - Wang,Yin, AU - Liu,Ji-Hong, Y1 - 2012/07/26/ PY - 2012/05/04/received PY - 2012/06/25/accepted PY - 2012/8/1/entrez PY - 2012/8/1/pubmed PY - 2013/4/10/medline SP - e41790 EP - e41790 JF - PloS one JO - PLoS One VL - 7 IS - 7 N2 - Citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating biotic stresses affecting the citrus industry. Meiwa kumquat (Fortunella crassifolia) is canker-resistant, while Newhall navel orange (Citrus sinensis Osbeck) is canker-sensitive. To understand the molecular mechanisms underlying the differences in responses to Xcc, transcriptomic profiles of these two genotypes following Xcc attack were compared by using the Affymetrix citrus genome GeneChip. A total of 794 and 1324 differentially expressed genes (DEGs) were identified as canker-responsive genes in Meiwa and Newhall, respectively. Of these, 230 genes were expressed in common between both genotypes, while 564 and 1094 genes were only significantly expressed in either Meiwa or Newhall. Gene ontology (GO) annotation and Singular Enrichment Analysis (SEA) of the DEGs showed that genes related to the cell wall and polysaccharide metabolism were induced for basic defense in both Meiwa and Newhall, such as chitinase, glucanase and thaumatin-like protein. Moreover, apart from inducing basic defense, Meiwa showed specially upregulated expression of several genes involved in the response to biotic stimulus, defense response, and cation binding as comparing with Newhall. And in Newhall, abundant photosynthesis-related genes were significantly down-regulated, which may be in order to ensure the basic defense. This study revealed different molecular responses to canker disease in Meiwa and Newhall, affording insight into the response to canker and providing valuable information for the identification of potential genes for engineering canker tolerance in the future. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/22848606/Different_transcriptional_response_to_Xanthomonas_citri_subsp__citri_between_kumquat_and_sweet_orange_with_contrasting_canker_tolerance_ L2 - https://dx.plos.org/10.1371/journal.pone.0041790 DB - PRIME DP - Unbound Medicine ER -