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Intraspecific variation of biological activities in venoms from wild and captive Bothrops jararaca.
J Toxicol Environ Health A. 2012; 75(16-17):1081-90.JT

Abstract

The venom of Bothrops jararaca is composed of complex mixture of molecules, mainly lectins, metalloproteinases, serinoproteinases, desintegrins, phospholipases, and peptides. This composition may vary according to the snake's age, gender, and region of origin. The aim of the was to determine individual variation in Bothrops jararaca venom in the Botucatu region, Sao Paulo State, Brazil, by means of enzymatic, biochemical, and pharmacological characterization, utilizing in vitro tests and biological assays. The activities were compared with those of Brazilian Reference Venom (BRV). Protein concentration varied between adult and juvenile groups. The electrophoretic profiles were similar, with molecular masses ranging between 25 and 50 kD, but with intraspecific variations. Reverse-phase high-performance liquid chromatography (RP-HPLC) revealed protein concentration differences. Coagulant activity did not differ significantly among adult groups, but there was a large variation between juvenile venom and BRV, which coagulated more extensively. Venoms from adults displayed greater hemorrhagic activity, especially in males recently obtained from the wild. In contrast, juveniles kept in captivity and adult males showed higher values. Edematogenic activity displayed an increase in edema in all groups. At the mean lethal dose (LD₅₀), toxicity varied significantly between groups, with venom from captive females being threefold more toxic than juvenile venom. Data illustrate the intra- and interspecific complexity that occurs in snake venoms, which may be attributed to ontogenetic, sexual, and environmental factors that affect variability in Bothrops jararaca venom. Further, it is proposed that Brazilian public health authorities document the constitution of pooled venom employed in the immunization of serum-producing animals due to this variability in venom properties. Given the large Brazilian territory, this variability requires regional monitoring and evaluation of the efficacy of bothropic antivenom in treatment of snakebite and consequent permanent sequelae observed.

Authors+Show Affiliations

Department of Tropical Diseases and Image Diagnosis, Botucatu Medical School, São Paulo State University-UNESP-Univ Estadual Paulista, Botucatu, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22852857

Citation

Saad, Eduardo, et al. "Intraspecific Variation of Biological Activities in Venoms From Wild and Captive Bothrops Jararaca." Journal of Toxicology and Environmental Health. Part A, vol. 75, no. 16-17, 2012, pp. 1081-90.
Saad E, Curtolo Barros L, Biscola N, et al. Intraspecific variation of biological activities in venoms from wild and captive Bothrops jararaca. J Toxicol Environ Health A. 2012;75(16-17):1081-90.
Saad, E., Curtolo Barros, L., Biscola, N., Pimenta, D. C., Barraviera, S. R., Barraviera, B., & Seabra Ferreira, R. (2012). Intraspecific variation of biological activities in venoms from wild and captive Bothrops jararaca. Journal of Toxicology and Environmental Health. Part A, 75(16-17), 1081-90. https://doi.org/10.1080/15287394.2012.697839
Saad E, et al. Intraspecific Variation of Biological Activities in Venoms From Wild and Captive Bothrops Jararaca. J Toxicol Environ Health A. 2012;75(16-17):1081-90. PubMed PMID: 22852857.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intraspecific variation of biological activities in venoms from wild and captive Bothrops jararaca. AU - Saad,Eduardo, AU - Curtolo Barros,Luciana, AU - Biscola,Natalia, AU - Pimenta,Daniel C, AU - Barraviera,Silvia R C S, AU - Barraviera,Benedito, AU - Seabra Ferreira,Rui,Jr PY - 2012/8/3/entrez PY - 2012/8/3/pubmed PY - 2012/10/10/medline SP - 1081 EP - 90 JF - Journal of toxicology and environmental health. Part A JO - J Toxicol Environ Health A VL - 75 IS - 16-17 N2 - The venom of Bothrops jararaca is composed of complex mixture of molecules, mainly lectins, metalloproteinases, serinoproteinases, desintegrins, phospholipases, and peptides. This composition may vary according to the snake's age, gender, and region of origin. The aim of the was to determine individual variation in Bothrops jararaca venom in the Botucatu region, Sao Paulo State, Brazil, by means of enzymatic, biochemical, and pharmacological characterization, utilizing in vitro tests and biological assays. The activities were compared with those of Brazilian Reference Venom (BRV). Protein concentration varied between adult and juvenile groups. The electrophoretic profiles were similar, with molecular masses ranging between 25 and 50 kD, but with intraspecific variations. Reverse-phase high-performance liquid chromatography (RP-HPLC) revealed protein concentration differences. Coagulant activity did not differ significantly among adult groups, but there was a large variation between juvenile venom and BRV, which coagulated more extensively. Venoms from adults displayed greater hemorrhagic activity, especially in males recently obtained from the wild. In contrast, juveniles kept in captivity and adult males showed higher values. Edematogenic activity displayed an increase in edema in all groups. At the mean lethal dose (LD₅₀), toxicity varied significantly between groups, with venom from captive females being threefold more toxic than juvenile venom. Data illustrate the intra- and interspecific complexity that occurs in snake venoms, which may be attributed to ontogenetic, sexual, and environmental factors that affect variability in Bothrops jararaca venom. Further, it is proposed that Brazilian public health authorities document the constitution of pooled venom employed in the immunization of serum-producing animals due to this variability in venom properties. Given the large Brazilian territory, this variability requires regional monitoring and evaluation of the efficacy of bothropic antivenom in treatment of snakebite and consequent permanent sequelae observed. SN - 1528-7394 UR - https://www.unboundmedicine.com/medline/citation/22852857/Intraspecific_variation_of_biological_activities_in_venoms_from_wild_and_captive_Bothrops_jararaca_ L2 - https://www.tandfonline.com/doi/full/10.1080/15287394.2012.697839 DB - PRIME DP - Unbound Medicine ER -