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The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacle Balanus (=Amphibalanus) amphitrite.
J Exp Biol. 2012 Nov 01; 215(Pt 21):3813-22.JE

Abstract

The barnacle Balanus amphitrite is among the most dominant fouling species on intertidal rocky shores in tropical and subtropical areas and is thus a target organism in antifouling research. After being released from adults, the swimming nauplius undertakes six molting cycles and then transforms into a cyprid. Using paired antennules, a competent cyprid actively explores and selects a suitable substratum for attachment and metamorphosis (collectively known as settlement). This selection process involves the reception of exogenous signals and subsequent endogenous signal transduction. To investigate the involvement of nitric oxide (NO) and cyclic GMP (cGMP) during larval settlement of B. amphitrite, we examined the effects of an NO donor and an NO scavenger, two nitric oxide synthase (NOS) inhibitors and a soluble guanylyl cyclase (sGC) inhibitor on settling cyprids. We found that the NO donor sodium nitroprusside (SNP) inhibited larval settlement in a dose-dependent manner. In contrast, both the NO scavenger carboxy-PTIO and the NOS inhibitors aminoguanidine hemisulfate (AGH) and S-methylisothiourea sulfate (SMIS) significantly accelerated larval settlement. Suppression of the downstream guanylyl cyclase (GC) activity using a GC-selective inhibitor ODQ could also significantly accelerate larval settlement. Interestingly, the settlement inhibition effects of SNP could be attenuated by ODQ at all concentrations tested. In the developmental expression profiling of NOS and sGC, the lowest expression of both genes was detected in the cyprid stage, a crucial stage for the larval decision to attach and metamorphose. In summary, we concluded that NO regulates larval settlement via mediating downstream cGMP signaling.

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Authors+Show Affiliations

Zhang Y
KAUST Global Collaborative Research Program, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, China.
He LS
No affiliation info available
Zhang G
No affiliation info available
Xu Y
No affiliation info available
Lee OO
No affiliation info available
Matsumura K
No affiliation info available
Qian PY
No affiliation info available

MeSH

AnimalsBenzoatesCyclic GMPGuanidinesGuanylate CyclaseImidazolesIsothiuroniumLarvaMetamorphosis, BiologicalNitric OxideNitric Oxide SynthaseNitroprussideOxadiazolesQuinoxalinesSignal TransductionThoracica

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22855617

Citation

Zhang, Yu, et al. "The Regulatory Role of the NO/cGMP Signal Transduction Cascade During Larval Attachment and Metamorphosis of the Barnacle Balanus (=Amphibalanus) Amphitrite." The Journal of Experimental Biology, vol. 215, no. Pt 21, 2012, pp. 3813-22.
Zhang Y, He LS, Zhang G, et al. The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacle Balanus (=Amphibalanus) amphitrite. J Exp Biol. 2012;215(Pt 21):3813-22.
Zhang, Y., He, L. S., Zhang, G., Xu, Y., Lee, O. O., Matsumura, K., & Qian, P. Y. (2012). The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacle Balanus (=Amphibalanus) amphitrite. The Journal of Experimental Biology, 215(Pt 21), 3813-22. https://doi.org/10.1242/jeb.070235
Zhang Y, et al. The Regulatory Role of the NO/cGMP Signal Transduction Cascade During Larval Attachment and Metamorphosis of the Barnacle Balanus (=Amphibalanus) Amphitrite. J Exp Biol. 2012 Nov 1;215(Pt 21):3813-22. PubMed PMID: 22855617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacle Balanus (=Amphibalanus) amphitrite. AU - Zhang,Yu, AU - He,Li-Sheng, AU - Zhang,Gen, AU - Xu,Ying, AU - Lee,On-On, AU - Matsumura,Kiyotaka, AU - Qian,Pei-Yuan, Y1 - 2012/08/01/ PY - 2012/8/3/entrez PY - 2012/8/3/pubmed PY - 2013/4/16/medline SP - 3813 EP - 22 JF - The Journal of experimental biology JO - J Exp Biol VL - 215 IS - Pt 21 N2 - The barnacle Balanus amphitrite is among the most dominant fouling species on intertidal rocky shores in tropical and subtropical areas and is thus a target organism in antifouling research. After being released from adults, the swimming nauplius undertakes six molting cycles and then transforms into a cyprid. Using paired antennules, a competent cyprid actively explores and selects a suitable substratum for attachment and metamorphosis (collectively known as settlement). This selection process involves the reception of exogenous signals and subsequent endogenous signal transduction. To investigate the involvement of nitric oxide (NO) and cyclic GMP (cGMP) during larval settlement of B. amphitrite, we examined the effects of an NO donor and an NO scavenger, two nitric oxide synthase (NOS) inhibitors and a soluble guanylyl cyclase (sGC) inhibitor on settling cyprids. We found that the NO donor sodium nitroprusside (SNP) inhibited larval settlement in a dose-dependent manner. In contrast, both the NO scavenger carboxy-PTIO and the NOS inhibitors aminoguanidine hemisulfate (AGH) and S-methylisothiourea sulfate (SMIS) significantly accelerated larval settlement. Suppression of the downstream guanylyl cyclase (GC) activity using a GC-selective inhibitor ODQ could also significantly accelerate larval settlement. Interestingly, the settlement inhibition effects of SNP could be attenuated by ODQ at all concentrations tested. In the developmental expression profiling of NOS and sGC, the lowest expression of both genes was detected in the cyprid stage, a crucial stage for the larval decision to attach and metamorphose. In summary, we concluded that NO regulates larval settlement via mediating downstream cGMP signaling. SN - 1477-9145 UR - https://www.unboundmedicine.com/medline/citation/22855617/The_regulatory_role_of_the_NO/cGMP_signal_transduction_cascade_during_larval_attachment_and_metamorphosis_of_the_barnacle_Balanus__=Amphibalanus__amphitrite_ L2 - http://jeb.biologists.org/cgi/pmidlookup?view=long&pmid=22855617 DB - PRIME DP - Unbound Medicine ER -