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In vitro anti-breast cancer activity of ethanolic extract of Wrightia tomentosa: role of pro-apoptotic effects of oleanolic acid and urosolic acid.
J Ethnopharmacol. 2012 Jun 26; 142(1):72-9.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Wrightia tomentosa Roem. & Schult. (Apocynaceae) is known in the traditional medicine for anti-cancer activity along with other broad indications like snake and scorpion bites, renal complications, menstrual disorders etc. However, the anti-cancer activity of this plant or its constituents has never been studied systematically in any cancer types so far.

AIM OF THE STUDY

To evaluate the anti-cancer activities of the ethanolic extract of W. tomentosa and identified constituent active molecule(s) against breast cancer.

MATERIAL AND METHODS

Powdered leaves of W. tomentosa were extracted with ethanol. The ethanolic extract, subsequent hexane fractions and fraction F-4 of W. tomentosa were tested for its anti-proliferative and pro-apoptotic effects in breast cancer cells MCF-7 and MDA-MB-231.

RESULTS

The ethanolic extract, subsequent hexane fractions and fraction F-4 of W. tomentosa inhibited the proliferation of human breast cancer cell lines, MCF-7 and MDA-MB-231. The fraction F-4 obtained from hexane fraction inhibited proliferation of MCF-7 and MDA-MB-231 cells in concentration and time dependent manner with IC₅₀ of 50 μg/ml and 30 μg/ml for 24 h, 28 μg/ml and 22 μg/ml for 48 h and 25 μg/ml and 20 μg/ml for 72 h respectively. The fraction F-4 induced G1 cell cycle arrest, reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential and subsequent apoptosis. Apoptosis is indicated in terms of increased Bax/Bcl-2 ratio, enhanced Annexin-V positivity, caspase 8 activation and DNA fragmentation. The active molecule isolated from fraction F-4, oleanolic acid and urosolic acid inhibited cell proliferation of MCF-7 and MDA-MB-231 cells at IC₅₀ value of 7.5 μM and 7.0 μM respectively, whereas there is devoid of significant cell inhibiting activity in non-cancer originated cells, HEK-293. In both MCF-7 and MDA-MB-231, oleanolic acid and urosolic acid induced cell cycle arrest and apoptosis as indicated by significant increase in Annexin-V positive apoptotic cell counts.

CONCLUSION

Our results suggest that W. tomentosa extracts has significant anti-cancer activity against breast cancer cells due to induction of apoptosis pathway. Olenolic and urosolic acid are important constituent molecules in the extract responsible for anti-cancer activity of W. tomentosa.

Authors+Show Affiliations

Division of Endocrinology, CSIR-Central Drug Research Institute-CDRI, U.P., Lucknow 226001, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22855944

Citation

Chakravarti, Bandana, et al. "In Vitro Anti-breast Cancer Activity of Ethanolic Extract of Wrightia Tomentosa: Role of Pro-apoptotic Effects of Oleanolic Acid and Urosolic Acid." Journal of Ethnopharmacology, vol. 142, no. 1, 2012, pp. 72-9.
Chakravarti B, Maurya R, Siddiqui JA, et al. In vitro anti-breast cancer activity of ethanolic extract of Wrightia tomentosa: role of pro-apoptotic effects of oleanolic acid and urosolic acid. J Ethnopharmacol. 2012;142(1):72-9.
Chakravarti, B., Maurya, R., Siddiqui, J. A., Bid, H. K., Rajendran, S. M., Yadav, P. P., & Konwar, R. (2012). In vitro anti-breast cancer activity of ethanolic extract of Wrightia tomentosa: role of pro-apoptotic effects of oleanolic acid and urosolic acid. Journal of Ethnopharmacology, 142(1), 72-9.
Chakravarti B, et al. In Vitro Anti-breast Cancer Activity of Ethanolic Extract of Wrightia Tomentosa: Role of Pro-apoptotic Effects of Oleanolic Acid and Urosolic Acid. J Ethnopharmacol. 2012 Jun 26;142(1):72-9. PubMed PMID: 22855944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro anti-breast cancer activity of ethanolic extract of Wrightia tomentosa: role of pro-apoptotic effects of oleanolic acid and urosolic acid. AU - Chakravarti,Bandana, AU - Maurya,Ranjani, AU - Siddiqui,Jawed Akhtar, AU - Bid,Hemant Kumar, AU - Rajendran,S M, AU - Yadav,Prem P, AU - Konwar,Rituraj, PY - 2012/8/3/entrez PY - 2012/8/3/pubmed PY - 2012/10/10/medline SP - 72 EP - 9 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 142 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Wrightia tomentosa Roem. & Schult. (Apocynaceae) is known in the traditional medicine for anti-cancer activity along with other broad indications like snake and scorpion bites, renal complications, menstrual disorders etc. However, the anti-cancer activity of this plant or its constituents has never been studied systematically in any cancer types so far. AIM OF THE STUDY: To evaluate the anti-cancer activities of the ethanolic extract of W. tomentosa and identified constituent active molecule(s) against breast cancer. MATERIAL AND METHODS: Powdered leaves of W. tomentosa were extracted with ethanol. The ethanolic extract, subsequent hexane fractions and fraction F-4 of W. tomentosa were tested for its anti-proliferative and pro-apoptotic effects in breast cancer cells MCF-7 and MDA-MB-231. RESULTS: The ethanolic extract, subsequent hexane fractions and fraction F-4 of W. tomentosa inhibited the proliferation of human breast cancer cell lines, MCF-7 and MDA-MB-231. The fraction F-4 obtained from hexane fraction inhibited proliferation of MCF-7 and MDA-MB-231 cells in concentration and time dependent manner with IC₅₀ of 50 μg/ml and 30 μg/ml for 24 h, 28 μg/ml and 22 μg/ml for 48 h and 25 μg/ml and 20 μg/ml for 72 h respectively. The fraction F-4 induced G1 cell cycle arrest, reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential and subsequent apoptosis. Apoptosis is indicated in terms of increased Bax/Bcl-2 ratio, enhanced Annexin-V positivity, caspase 8 activation and DNA fragmentation. The active molecule isolated from fraction F-4, oleanolic acid and urosolic acid inhibited cell proliferation of MCF-7 and MDA-MB-231 cells at IC₅₀ value of 7.5 μM and 7.0 μM respectively, whereas there is devoid of significant cell inhibiting activity in non-cancer originated cells, HEK-293. In both MCF-7 and MDA-MB-231, oleanolic acid and urosolic acid induced cell cycle arrest and apoptosis as indicated by significant increase in Annexin-V positive apoptotic cell counts. CONCLUSION: Our results suggest that W. tomentosa extracts has significant anti-cancer activity against breast cancer cells due to induction of apoptosis pathway. Olenolic and urosolic acid are important constituent molecules in the extract responsible for anti-cancer activity of W. tomentosa. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22855944/In_vitro_anti_breast_cancer_activity_of_ethanolic_extract_of_Wrightia_tomentosa:_role_of_pro_apoptotic_effects_of_oleanolic_acid_and_urosolic_acid_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00242-5 DB - PRIME DP - Unbound Medicine ER -