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An evaluation of the antinociceptive effects of Phα1β, a neurotoxin from the spider Phoneutria nigriventer, and ω-conotoxin MVIIA, a cone snail Conus magus toxin, in rat model of inflammatory and neuropathic pain.
Cell Mol Neurobiol. 2013 Jan; 33(1):59-67.CM

Abstract

Voltage-sensitive calcium channels (VSCCs) underlie cell excitability and are involved in the mechanisms that generate and maintain neuropathic and inflammatory pain. We evaluated in rats the effects of two VSCC blockers, ω-conotoxin MVIIA and Phα1β, in models of inflammatory and neuropathic pain induced with complete Freund's adjuvant (CFA) and chronic constrictive injury (CCI), respectively. We also evaluated the effects of the toxins on capsaicin-induced Ca(2+) influx in dorsal root ganglion (DRG) neurons obtained from rats exposed to both models of pain. A single intrathecal injection of Phα1β reversibly inhibits CFA and CCI-induced mechanical hyperalgesia longer than a single injection of ω-conotoxin MVIIA. Phα1β and MVIIA also inhibited capsaicin-induced Ca(2+) influx in DRG neurons. The inhibitory effect of Phα1β on capsaicin-induced calcium transients in DRG neurons was greater in the CFA model of pain, while the inhibitory effect of ω-conotoxin MVIIA was greater in the CCI model. The management of chronic inflammatory and neuropathic pain is still a major challenge for clinicians. Phα1β, a reversible inhibitor of VSCCs with a preference for N-type Ca(2+) channels, has potential as a novel therapeutic agent for inflammatory and neuropathic pain. Clinical studies are necessary to establish the role of Phα1β in the treatment of chronic pain.

Authors+Show Affiliations

Programa de Pós-Graduação em Medicina Molecular, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22869352

Citation

de Souza, Alessandra Hubner, et al. "An Evaluation of the Antinociceptive Effects of Phα1β, a Neurotoxin From the Spider Phoneutria Nigriventer, and Ω-conotoxin MVIIA, a Cone Snail Conus Magus Toxin, in Rat Model of Inflammatory and Neuropathic Pain." Cellular and Molecular Neurobiology, vol. 33, no. 1, 2013, pp. 59-67.
de Souza AH, Castro CJ, Rigo FK, et al. An evaluation of the antinociceptive effects of Phα1β, a neurotoxin from the spider Phoneutria nigriventer, and ω-conotoxin MVIIA, a cone snail Conus magus toxin, in rat model of inflammatory and neuropathic pain. Cell Mol Neurobiol. 2013;33(1):59-67.
de Souza, A. H., Castro, C. J., Rigo, F. K., de Oliveira, S. M., Gomez, R. S., Diniz, D. M., Borges, M. H., Cordeiro, M. N., Silva, M. A., Ferreira, J., & Gomez, M. V. (2013). An evaluation of the antinociceptive effects of Phα1β, a neurotoxin from the spider Phoneutria nigriventer, and ω-conotoxin MVIIA, a cone snail Conus magus toxin, in rat model of inflammatory and neuropathic pain. Cellular and Molecular Neurobiology, 33(1), 59-67. https://doi.org/10.1007/s10571-012-9871-x
de Souza AH, et al. An Evaluation of the Antinociceptive Effects of Phα1β, a Neurotoxin From the Spider Phoneutria Nigriventer, and Ω-conotoxin MVIIA, a Cone Snail Conus Magus Toxin, in Rat Model of Inflammatory and Neuropathic Pain. Cell Mol Neurobiol. 2013;33(1):59-67. PubMed PMID: 22869352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An evaluation of the antinociceptive effects of Phα1β, a neurotoxin from the spider Phoneutria nigriventer, and ω-conotoxin MVIIA, a cone snail Conus magus toxin, in rat model of inflammatory and neuropathic pain. AU - de Souza,Alessandra Hubner, AU - Castro,Célio J,Jr AU - Rigo,Flavia Karine, AU - de Oliveira,Sara Marchesan, AU - Gomez,Renato Santiago, AU - Diniz,Danuza Montijo, AU - Borges,Marcia Helena, AU - Cordeiro,Marta Nascimento, AU - Silva,Marco Aurélio Romano, AU - Ferreira,Juliano, AU - Gomez,Marcus Vinicius, Y1 - 2012/08/07/ PY - 2012/03/09/received PY - 2012/07/25/accepted PY - 2012/8/8/entrez PY - 2012/8/8/pubmed PY - 2013/10/22/medline SP - 59 EP - 67 JF - Cellular and molecular neurobiology JO - Cell Mol Neurobiol VL - 33 IS - 1 N2 - Voltage-sensitive calcium channels (VSCCs) underlie cell excitability and are involved in the mechanisms that generate and maintain neuropathic and inflammatory pain. We evaluated in rats the effects of two VSCC blockers, ω-conotoxin MVIIA and Phα1β, in models of inflammatory and neuropathic pain induced with complete Freund's adjuvant (CFA) and chronic constrictive injury (CCI), respectively. We also evaluated the effects of the toxins on capsaicin-induced Ca(2+) influx in dorsal root ganglion (DRG) neurons obtained from rats exposed to both models of pain. A single intrathecal injection of Phα1β reversibly inhibits CFA and CCI-induced mechanical hyperalgesia longer than a single injection of ω-conotoxin MVIIA. Phα1β and MVIIA also inhibited capsaicin-induced Ca(2+) influx in DRG neurons. The inhibitory effect of Phα1β on capsaicin-induced calcium transients in DRG neurons was greater in the CFA model of pain, while the inhibitory effect of ω-conotoxin MVIIA was greater in the CCI model. The management of chronic inflammatory and neuropathic pain is still a major challenge for clinicians. Phα1β, a reversible inhibitor of VSCCs with a preference for N-type Ca(2+) channels, has potential as a novel therapeutic agent for inflammatory and neuropathic pain. Clinical studies are necessary to establish the role of Phα1β in the treatment of chronic pain. SN - 1573-6830 UR - https://www.unboundmedicine.com/medline/citation/22869352/An_evaluation_of_the_antinociceptive_effects_of_Phα1β_a_neurotoxin_from_the_spider_Phoneutria_nigriventer_and_ω_conotoxin_MVIIA_a_cone_snail_Conus_magus_toxin_in_rat_model_of_inflammatory_and_neuropathic_pain_ L2 - https://doi.org/10.1007/s10571-012-9871-x DB - PRIME DP - Unbound Medicine ER -