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Lectin microarrays differentiate carcinoma cells from reactive mesothelial cells in pleural effusions.
Cytotechnology. 2013 May; 65(3):355-62.C

Abstract

The aim of this study was to evaluate the diagnostic utility of lectin microarrays in pleural effusions of patients with lung cancer. A lectin microarray, LTL, PSA, LCA, UEA-1, AAL, MAL-I, MAL-II, SNA, WGA, ECL, DSA, STL, SWGA, HPA, ConA, GNA, HHL, BPL, EEL, Jacalin, WFA, ACL, MPL, DBA, SBA, was used to determine the glycoprotein profile of cells in pleural effusions from patients with lung cancer (54 cases), and with benign lung disease (54 cases). The A549 cell line, used as an experimental control, was positive for AAL, MAL-I, WGA, STL, Jacalin and ACL binding. Adenocarcinoma cells in pleural effusions were positive for ECL, DSA, AAL, MAL-I, WGA, STL, Jacalin, and ACL binding. AAL, WGA, and ACL positive binding was the most common, found in 54, 48, and 38 samples, respectively. ECL and DSA binding was positive in only 4 samples. In comparison, reactive mesothelial cells displayed positive binding for all markers in the microarray panel. SNA and AAL positive binding was detected in the majority of samples; 50/54 and 48/54 samples, respectively. Positive binding of DBA, MAL-II and EEL was present in only 2, 4 and 4 samples, respectively. SNA binding had the highest sensitivity (92.6 %), specificity (100 %), and accuracy (96.3 %). SNA may be used as a biomarker to distinguish reactive mesothelial cells from adenocarcinoma cells. The lectin microarrays proved able to distinguish carcinoma cells from reactive mesothelial cells in pleural effusions.

Authors+Show Affiliations

Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, 110001, China, shiyuqing101@sina.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22875282

Citation

Shi, Yu-Qing, et al. "Lectin Microarrays Differentiate Carcinoma Cells From Reactive Mesothelial Cells in Pleural Effusions." Cytotechnology, vol. 65, no. 3, 2013, pp. 355-62.
Shi YQ, He Q, Zhao YJ, et al. Lectin microarrays differentiate carcinoma cells from reactive mesothelial cells in pleural effusions. Cytotechnology. 2013;65(3):355-62.
Shi, Y. Q., He, Q., Zhao, Y. J., Wang, E. H., & Wu, G. P. (2013). Lectin microarrays differentiate carcinoma cells from reactive mesothelial cells in pleural effusions. Cytotechnology, 65(3), 355-62. https://doi.org/10.1007/s10616-012-9474-x
Shi YQ, et al. Lectin Microarrays Differentiate Carcinoma Cells From Reactive Mesothelial Cells in Pleural Effusions. Cytotechnology. 2013;65(3):355-62. PubMed PMID: 22875282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lectin microarrays differentiate carcinoma cells from reactive mesothelial cells in pleural effusions. AU - Shi,Yu-Qing, AU - He,Qun, AU - Zhao,Yu-Jie, AU - Wang,En-Hua, AU - Wu,Guang-Ping, Y1 - 2012/08/09/ PY - 2012/01/05/received PY - 2012/06/05/accepted PY - 2012/8/10/entrez PY - 2012/8/10/pubmed PY - 2012/8/10/medline SP - 355 EP - 62 JF - Cytotechnology JO - Cytotechnology VL - 65 IS - 3 N2 - The aim of this study was to evaluate the diagnostic utility of lectin microarrays in pleural effusions of patients with lung cancer. A lectin microarray, LTL, PSA, LCA, UEA-1, AAL, MAL-I, MAL-II, SNA, WGA, ECL, DSA, STL, SWGA, HPA, ConA, GNA, HHL, BPL, EEL, Jacalin, WFA, ACL, MPL, DBA, SBA, was used to determine the glycoprotein profile of cells in pleural effusions from patients with lung cancer (54 cases), and with benign lung disease (54 cases). The A549 cell line, used as an experimental control, was positive for AAL, MAL-I, WGA, STL, Jacalin and ACL binding. Adenocarcinoma cells in pleural effusions were positive for ECL, DSA, AAL, MAL-I, WGA, STL, Jacalin, and ACL binding. AAL, WGA, and ACL positive binding was the most common, found in 54, 48, and 38 samples, respectively. ECL and DSA binding was positive in only 4 samples. In comparison, reactive mesothelial cells displayed positive binding for all markers in the microarray panel. SNA and AAL positive binding was detected in the majority of samples; 50/54 and 48/54 samples, respectively. Positive binding of DBA, MAL-II and EEL was present in only 2, 4 and 4 samples, respectively. SNA binding had the highest sensitivity (92.6 %), specificity (100 %), and accuracy (96.3 %). SNA may be used as a biomarker to distinguish reactive mesothelial cells from adenocarcinoma cells. The lectin microarrays proved able to distinguish carcinoma cells from reactive mesothelial cells in pleural effusions. SN - 0920-9069 UR - https://www.unboundmedicine.com/medline/citation/22875282/Lectin_microarrays_differentiate_carcinoma_cells_from_reactive_mesothelial_cells_in_pleural_effusions_ L2 - https://dx.doi.org/10.1007/s10616-012-9474-x DB - PRIME DP - Unbound Medicine ER -
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