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Detection of mutations in the gyrA and parC genes in Escherichia coli isolates carrying plasmid-mediated quinolone resistance genes from diseased food-producing animals.
J Med Microbiol. 2012 Nov; 61(Pt 11):1591-1599.JM

Abstract

In this study, the prevalence of plasmid-mediated quinolone resistance (PMQR) was investigated in 495 Escherichia coli isolates from diseased food-producing animals in Guangdong province, China. The quinolone resistance-determining regions (QRDRs) of the gyrA and parC genes were analysed for mutations in 55 isolates harbouring only oqxAB and all isolates harbouring other PMQR genes. Overall, 282 (57.0 %) E. coli isolates had at least one PMQR gene. oqxAB was detected in 215 isolates and predominated the PMQR genes, followed by qnrS (63 isolates), aac(6')-Ib-cr (56 isolates), qnrB (39 isolates) and qepA (18 isolates). qnrA, qnrC and qnrD were not found in any of the isolates. The rates of resistance to ciprofloxacin, enrofloxacin, levofloxacin and nalidixic acid were 75.2, 81.0, 70.5 and 97.4 %, respectively, among the 495 isolates. Eight types of mutation in gyrA were detected in 154 PMQR-positive isolates, and 147 isolates were found to have mutations in parC. PFGE analysis indicated that the PMQR-positive E. coli isolates were genetically diverse. This study demonstrated that the number of mutations in QRDRs of gyrA and/or parC was significantly associated with the MICs of quinolones (P<0.01). The rates of resistance to ciprofloxacin, enrofloxacin and nalidixic acid in PMQR-positive isolates were significantly higher than those in PMQR-negative isolates (P<0.05). In addition, the prevalence of oqxAB had significant Spearman correlation coefficients in relation to the MICs of all four tested quinolones (P<0.01).

Authors+Show Affiliations

College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, PR China.College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, PR China.College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, PR China.College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, PR China.College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, PR China.College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, PR China.College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, PR China.College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, PR China.College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, PR China.College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, PR China.College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, PR China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22878251

Citation

Liu, Bao-Tao, et al. "Detection of Mutations in the gyrA and parC Genes in Escherichia Coli Isolates Carrying Plasmid-mediated Quinolone Resistance Genes From Diseased Food-producing Animals." Journal of Medical Microbiology, vol. 61, no. Pt 11, 2012, pp. 1591-1599.
Liu BT, Liao XP, Yang SS, et al. Detection of mutations in the gyrA and parC genes in Escherichia coli isolates carrying plasmid-mediated quinolone resistance genes from diseased food-producing animals. J Med Microbiol. 2012;61(Pt 11):1591-1599.
Liu, B. T., Liao, X. P., Yang, S. S., Wang, X. M., Li, L. L., Sun, J., Yang, Y. R., Fang, L. X., Li, L., Zhao, D. H., & Liu, Y. H. (2012). Detection of mutations in the gyrA and parC genes in Escherichia coli isolates carrying plasmid-mediated quinolone resistance genes from diseased food-producing animals. Journal of Medical Microbiology, 61(Pt 11), 1591-1599. https://doi.org/10.1099/jmm.0.043307-0
Liu BT, et al. Detection of Mutations in the gyrA and parC Genes in Escherichia Coli Isolates Carrying Plasmid-mediated Quinolone Resistance Genes From Diseased Food-producing Animals. J Med Microbiol. 2012;61(Pt 11):1591-1599. PubMed PMID: 22878251.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of mutations in the gyrA and parC genes in Escherichia coli isolates carrying plasmid-mediated quinolone resistance genes from diseased food-producing animals. AU - Liu,Bao-Tao, AU - Liao,Xiao-Ping, AU - Yang,Shou-Shen, AU - Wang,Xiu-Mei, AU - Li,Lu-Lu, AU - Sun,Jian, AU - Yang,Yu-Rong, AU - Fang,Liang-Xing, AU - Li,Liang, AU - Zhao,Dong-Hao, AU - Liu,Ya-Hong, Y1 - 2012/08/09/ PY - 2012/8/11/entrez PY - 2012/8/11/pubmed PY - 2013/1/4/medline SP - 1591 EP - 1599 JF - Journal of medical microbiology JO - J Med Microbiol VL - 61 IS - Pt 11 N2 - In this study, the prevalence of plasmid-mediated quinolone resistance (PMQR) was investigated in 495 Escherichia coli isolates from diseased food-producing animals in Guangdong province, China. The quinolone resistance-determining regions (QRDRs) of the gyrA and parC genes were analysed for mutations in 55 isolates harbouring only oqxAB and all isolates harbouring other PMQR genes. Overall, 282 (57.0 %) E. coli isolates had at least one PMQR gene. oqxAB was detected in 215 isolates and predominated the PMQR genes, followed by qnrS (63 isolates), aac(6')-Ib-cr (56 isolates), qnrB (39 isolates) and qepA (18 isolates). qnrA, qnrC and qnrD were not found in any of the isolates. The rates of resistance to ciprofloxacin, enrofloxacin, levofloxacin and nalidixic acid were 75.2, 81.0, 70.5 and 97.4 %, respectively, among the 495 isolates. Eight types of mutation in gyrA were detected in 154 PMQR-positive isolates, and 147 isolates were found to have mutations in parC. PFGE analysis indicated that the PMQR-positive E. coli isolates were genetically diverse. This study demonstrated that the number of mutations in QRDRs of gyrA and/or parC was significantly associated with the MICs of quinolones (P<0.01). The rates of resistance to ciprofloxacin, enrofloxacin and nalidixic acid in PMQR-positive isolates were significantly higher than those in PMQR-negative isolates (P<0.05). In addition, the prevalence of oqxAB had significant Spearman correlation coefficients in relation to the MICs of all four tested quinolones (P<0.01). SN - 1473-5644 UR - https://www.unboundmedicine.com/medline/citation/22878251/Detection_of_mutations_in_the_gyrA_and_parC_genes_in_Escherichia_coli_isolates_carrying_plasmid_mediated_quinolone_resistance_genes_from_diseased_food_producing_animals_ L2 - http://jmm.microbiologyresearch.org/pubmed/content/journal/jmm/10.1099/jmm.0.043307-0 DB - PRIME DP - Unbound Medicine ER -