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Tracking retinal microgliosis in models of retinal ganglion cell damage.
Invest Ophthalmol Vis Sci. 2012 Sep 14; 53(10):6254-62.IO

Abstract

PURPOSE

To investigate the longitudinal profiles of microgliosis after optic nerve injury induced by optic nerve crush and acute elevation of intraocular pressure (IOP).

METHODS

A confocal scanning laser ophthalmoscope was used to image the retinal microglia of the CX3CR1(GFP/+) transgenic mice in vivo at baseline, 3 days and then weekly for 4 weeks after optic nerve crush (n = 3), and after elevating the IOP to 110 mm Hg for 30 (n = 3) or 60 (n = 3) minutes.

RESULTS

After optic nerve crush, the density of microglia increased by 2.43 ± 0.19-fold at week 1 and then gradually declined with 2.04 ± 0.24-, 1.69 ± 0.25-, and 1.29 ± 0.11-fold increases at week 2, 3, and 4, respectively. Microgliosis followed a similar pattern after acute IOP elevation and the increase in microglia was associated with the duration of IOP elevation. There were 1.35 ± 0.17- and 2.03 ± 0.08-fold increases in microglia at week 1, and 1.15 ± 0.11- and 1.11 ± 0.10-fold increases at week 4, after 30 and 60 minutes of acute IOP elevation, respectively. The morphology of microglia changed from ramified to ameboid form in 1 week, and then returned to ramified form in the subsequent weeks. There was a significant negative association between the number of surviving retinal ganglion cells (RGCs) and the extent of microgliosis during the follow-up period (R² = 0.72, P = 0.004).

CONCLUSIONS

Longitudinal in vivo imaging of the retinal microglia can provide an effective approach to study microgliosis and its association with RGC degeneration.

Authors+Show Affiliations

Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22879415

Citation

Liu, Shu, et al. "Tracking Retinal Microgliosis in Models of Retinal Ganglion Cell Damage." Investigative Ophthalmology & Visual Science, vol. 53, no. 10, 2012, pp. 6254-62.
Liu S, Li ZW, Weinreb RN, et al. Tracking retinal microgliosis in models of retinal ganglion cell damage. Invest Ophthalmol Vis Sci. 2012;53(10):6254-62.
Liu, S., Li, Z. W., Weinreb, R. N., Xu, G., Lindsey, J. D., Ye, C., Yung, W. H., Pang, C. P., Lam, D. S., & Leung, C. K. (2012). Tracking retinal microgliosis in models of retinal ganglion cell damage. Investigative Ophthalmology & Visual Science, 53(10), 6254-62. https://doi.org/10.1167/iovs.12-9450
Liu S, et al. Tracking Retinal Microgliosis in Models of Retinal Ganglion Cell Damage. Invest Ophthalmol Vis Sci. 2012 Sep 14;53(10):6254-62. PubMed PMID: 22879415.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tracking retinal microgliosis in models of retinal ganglion cell damage. AU - Liu,Shu, AU - Li,Zhi-wai, AU - Weinreb,Robert N, AU - Xu,Guihua, AU - Lindsey,James D, AU - Ye,Cong, AU - Yung,Wing-ho, AU - Pang,Chi-Pui, AU - Lam,Dennis Shun Chiu, AU - Leung,Christopher Kai-shun, Y1 - 2012/09/14/ PY - 2012/8/11/entrez PY - 2012/8/11/pubmed PY - 2012/12/10/medline SP - 6254 EP - 62 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 53 IS - 10 N2 - PURPOSE: To investigate the longitudinal profiles of microgliosis after optic nerve injury induced by optic nerve crush and acute elevation of intraocular pressure (IOP). METHODS: A confocal scanning laser ophthalmoscope was used to image the retinal microglia of the CX3CR1(GFP/+) transgenic mice in vivo at baseline, 3 days and then weekly for 4 weeks after optic nerve crush (n = 3), and after elevating the IOP to 110 mm Hg for 30 (n = 3) or 60 (n = 3) minutes. RESULTS: After optic nerve crush, the density of microglia increased by 2.43 ± 0.19-fold at week 1 and then gradually declined with 2.04 ± 0.24-, 1.69 ± 0.25-, and 1.29 ± 0.11-fold increases at week 2, 3, and 4, respectively. Microgliosis followed a similar pattern after acute IOP elevation and the increase in microglia was associated with the duration of IOP elevation. There were 1.35 ± 0.17- and 2.03 ± 0.08-fold increases in microglia at week 1, and 1.15 ± 0.11- and 1.11 ± 0.10-fold increases at week 4, after 30 and 60 minutes of acute IOP elevation, respectively. The morphology of microglia changed from ramified to ameboid form in 1 week, and then returned to ramified form in the subsequent weeks. There was a significant negative association between the number of surviving retinal ganglion cells (RGCs) and the extent of microgliosis during the follow-up period (R² = 0.72, P = 0.004). CONCLUSIONS: Longitudinal in vivo imaging of the retinal microglia can provide an effective approach to study microgliosis and its association with RGC degeneration. SN - 1552-5783 UR - https://www.unboundmedicine.com/medline/citation/22879415/Tracking_retinal_microgliosis_in_models_of_retinal_ganglion_cell_damage_ L2 - https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.12-9450 DB - PRIME DP - Unbound Medicine ER -