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Investigation of optimal extraction, antioxidant, and antimicrobial activities of Achillea biebersteinii and A. wilhelmsii.
Pharm Biol. 2012 Sep; 50(9):1168-76.PB

Abstract

OBJECTIVE

Achillea species are endowed with multiple biological activities including antioxidant properties. However, no study has yet investigated the impact of extraction method and pH on the biological activities of these plants. The present study aimed to investigate the antioxidant and antimicrobial effects of methanol extracts from the aerial parts of the species Achillea biebersteinii Afan and Achillea wilhelmsii C. Koch (Asteraceae). In addition, the impact of extraction method and pH on these biological activities was evaluated.

MATERIALS AND METHODS

Methanol extracts of A. biebersteinii and A. wilhelmsii were prepared using classical maceration and high-intensity ultrasound methods. Ultrasound-assisted extraction was performed at three different pH values: 5.7, 6.3 and 6.9.

RESULTS

Total phenolic compounds (range: 20.16-108.54 vs. 17.18-59.61 mg gallic acid equivalent/g sample in A. biebersteinii and A. wilhelmsii, respectively), total flavonoids (range: 8.33-12.97 vs. 7.79-9.41 mg catechin equivalent/g sample), 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (IC₅₀: 40.63-346.34 vs. 84.02-462.41) and reducing power (IC₅₀: 504.44-4406.67 vs. 1710.00-5501.67) were significantly higher in A. biebersteinii vs. A. wilhelmsii and ultrasound-assisted vs. classical maceration extracts of both species. The aforementioned items were higher at pH = 6.3, followed by pHs of 6.9 and 5.7, respectively. Overall, A. biebersteinii extracts were more active against all of the tested microorganisms than A. wilhelmsii. Sensitivities of Gram-positive bacteria were higher for both Achillea extracts than the Gram-negative bacteria. No observable inhibitory activity was found from different extracts against Aspergillus niger.

CONCLUSION

The findings of the present study suggest that methanol extracts of A. biebersteinii and A. wilhelmsii possess antioxidant and antimicrobial activity, being higher in the former. Ultrasound-assisted extraction and pH of 6.3 have significant augmenting impact on the total phenolic and flavonoid content as well as antioxidant activities of both species.

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Authors+Show Affiliations

Bashi DS
Department of Food Science and Technology, Sabzevar Branch, Islamic Azad Univereity, Sabzevar, Iran. salarbashi@iaus.ac.ir
Fazly Bazzaz BS
No affiliation info available
Sahebkar A
No affiliation info available
Karimkhani MM
No affiliation info available
Ahmadi A
No affiliation info available

MeSH

AchilleaAnti-Bacterial AgentsAntifungal AgentsAntioxidantsCandida albicansFlavonoidsFree Radical ScavengersGram-Negative BacteriaGram-Positive BacteriaHydrogen-Ion ConcentrationIranMethanolMicrobial Sensitivity TestsPhenolsPlant Components, AerialPlant ExtractsSolventsUltrasonics

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

22881142

Citation

Bashi, Davoud Salar, et al. "Investigation of Optimal Extraction, Antioxidant, and Antimicrobial Activities of Achillea Biebersteinii and A. Wilhelmsii." Pharmaceutical Biology, vol. 50, no. 9, 2012, pp. 1168-76.
Bashi DS, Fazly Bazzaz BS, Sahebkar A, et al. Investigation of optimal extraction, antioxidant, and antimicrobial activities of Achillea biebersteinii and A. wilhelmsii. Pharm Biol. 2012;50(9):1168-76.
Bashi, D. S., Fazly Bazzaz, B. S., Sahebkar, A., Karimkhani, M. M., & Ahmadi, A. (2012). Investigation of optimal extraction, antioxidant, and antimicrobial activities of Achillea biebersteinii and A. wilhelmsii. Pharmaceutical Biology, 50(9), 1168-76. https://doi.org/10.3109/13880209.2012.662235
Bashi DS, et al. Investigation of Optimal Extraction, Antioxidant, and Antimicrobial Activities of Achillea Biebersteinii and A. Wilhelmsii. Pharm Biol. 2012;50(9):1168-76. PubMed PMID: 22881142.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigation of optimal extraction, antioxidant, and antimicrobial activities of Achillea biebersteinii and A. wilhelmsii. AU - Bashi,Davoud Salar, AU - Fazly Bazzaz,Bibi Sedigheh, AU - Sahebkar,Amirhossein, AU - Karimkhani,Mohamad Mahdi, AU - Ahmadi,Abbas, PY - 2012/8/14/entrez PY - 2012/8/14/pubmed PY - 2013/4/26/medline SP - 1168 EP - 76 JF - Pharmaceutical biology JO - Pharm Biol VL - 50 IS - 9 N2 - OBJECTIVE: Achillea species are endowed with multiple biological activities including antioxidant properties. However, no study has yet investigated the impact of extraction method and pH on the biological activities of these plants. The present study aimed to investigate the antioxidant and antimicrobial effects of methanol extracts from the aerial parts of the species Achillea biebersteinii Afan and Achillea wilhelmsii C. Koch (Asteraceae). In addition, the impact of extraction method and pH on these biological activities was evaluated. MATERIALS AND METHODS: Methanol extracts of A. biebersteinii and A. wilhelmsii were prepared using classical maceration and high-intensity ultrasound methods. Ultrasound-assisted extraction was performed at three different pH values: 5.7, 6.3 and 6.9. RESULTS: Total phenolic compounds (range: 20.16-108.54 vs. 17.18-59.61 mg gallic acid equivalent/g sample in A. biebersteinii and A. wilhelmsii, respectively), total flavonoids (range: 8.33-12.97 vs. 7.79-9.41 mg catechin equivalent/g sample), 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (IC₅₀: 40.63-346.34 vs. 84.02-462.41) and reducing power (IC₅₀: 504.44-4406.67 vs. 1710.00-5501.67) were significantly higher in A. biebersteinii vs. A. wilhelmsii and ultrasound-assisted vs. classical maceration extracts of both species. The aforementioned items were higher at pH = 6.3, followed by pHs of 6.9 and 5.7, respectively. Overall, A. biebersteinii extracts were more active against all of the tested microorganisms than A. wilhelmsii. Sensitivities of Gram-positive bacteria were higher for both Achillea extracts than the Gram-negative bacteria. No observable inhibitory activity was found from different extracts against Aspergillus niger. CONCLUSION: The findings of the present study suggest that methanol extracts of A. biebersteinii and A. wilhelmsii possess antioxidant and antimicrobial activity, being higher in the former. Ultrasound-assisted extraction and pH of 6.3 have significant augmenting impact on the total phenolic and flavonoid content as well as antioxidant activities of both species. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/22881142/Investigation_of_optimal_extraction_antioxidant_and_antimicrobial_activities_of_Achillea_biebersteinii_and_A__wilhelmsii_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2012.662235 DB - PRIME DP - Unbound Medicine ER -