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Prevalence and diversity of Synergistetes taxa in periodontal health and disease.
J Periodontal Res. 2013 Apr; 48(2):159-68.JP

Abstract

BACKGROUND AND OBJECTIVE

Members of the phylum Synergistetes have previously been identified within periodontitis subgingival plaque and are considered putative periodontopathogens. This study compared the diversity of subginigval Synergistetes in a cohort of subjects with periodontitis (n = 10) vs. periodontitis-free controls (n = 10).

MATERIAL AND METHODS

Pooled subgingival plaque samples from all deep periodontal pockets or all sulci were collected from the periodontitis and periodontitis-free subjects, respectively. Bacterial 16S rRNA genes were PCR-amplified from purified subgingival plaque DNA using a Synergistetes 'selective' primer set. PCR products were cloned and sequenced to analyze the prevalence and diversity of Synergistetes operational taxonomic units (OTUs) present in plaque samples of both subject groups.

RESULTS

A total of 1030 non-chimeric 16S rRNA clones were obtained, of which 162 corresponded to members of the phylum Synergistetes. A significantly larger number of Synergistetes clones were obtained from periodontitis subgingival plaque than from periodontitis-free controls (25.4% vs. 5.9%, p < 0.001). All Synergistetes clones corresponded to cluster A oral Synergistetes, and fell into 31 OTUs (99% sequence identity cut-off). Twenty-nine Synergistetes OTUs were detected in the periodontitis group while eight were detected in the periodontitis-free group (p < 0.001). Five Synergistetes OTUs; including one OTU corresponding to the recently-characterized species Fretibacterium fastidiosum, were more prevalent in the periodontitis subjects (p < 0.05).

CONCLUSION

OTUs belonging to oral Synergistetes cluster A were more readily detectable and were more diverse in subgingival plaque from periodontitis subjects compared with periodontitis-free controls. Specific Synergistetes OTUs appear to be associated with periodontitis.

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Authors+Show Affiliations

You M
Oral Diagnosis and Polyclinics, Prince Philip Dental Hospital, The University of Hong Kong, Hong Kong SAR, China.
Mo S
No affiliation info available
Watt RM
No affiliation info available
Leung WK
No affiliation info available

MeSH

ActinobacteriaAdultDNA PrimersDNA, BacterialDental PlaqueFemaleFusobacteriaGingival HemorrhageGram-Negative Anaerobic BacteriaHumansMaleMiddle AgedPeriodontal PocketPeriodontitisPeriodontiumPhylogenyPolymerase Chain ReactionProteobacteriaRNA, BacterialRNA, Ribosomal, 16SSpirochaetales

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22881378

Citation

You, M, et al. "Prevalence and Diversity of Synergistetes Taxa in Periodontal Health and Disease." Journal of Periodontal Research, vol. 48, no. 2, 2013, pp. 159-68.
You M, Mo S, Watt RM, et al. Prevalence and diversity of Synergistetes taxa in periodontal health and disease. J Periodontal Res. 2013;48(2):159-68.
You, M., Mo, S., Watt, R. M., & Leung, W. K. (2013). Prevalence and diversity of Synergistetes taxa in periodontal health and disease. Journal of Periodontal Research, 48(2), 159-68. https://doi.org/10.1111/j.1600-0765.2012.01516.x
You M, et al. Prevalence and Diversity of Synergistetes Taxa in Periodontal Health and Disease. J Periodontal Res. 2013;48(2):159-68. PubMed PMID: 22881378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence and diversity of Synergistetes taxa in periodontal health and disease. AU - You,M, AU - Mo,S, AU - Watt,R M, AU - Leung,W K, Y1 - 2012/08/06/ PY - 2012/06/26/accepted PY - 2012/8/14/entrez PY - 2012/8/14/pubmed PY - 2013/8/24/medline SP - 159 EP - 68 JF - Journal of periodontal research JO - J Periodontal Res VL - 48 IS - 2 N2 - BACKGROUND AND OBJECTIVE: Members of the phylum Synergistetes have previously been identified within periodontitis subgingival plaque and are considered putative periodontopathogens. This study compared the diversity of subginigval Synergistetes in a cohort of subjects with periodontitis (n = 10) vs. periodontitis-free controls (n = 10). MATERIAL AND METHODS: Pooled subgingival plaque samples from all deep periodontal pockets or all sulci were collected from the periodontitis and periodontitis-free subjects, respectively. Bacterial 16S rRNA genes were PCR-amplified from purified subgingival plaque DNA using a Synergistetes 'selective' primer set. PCR products were cloned and sequenced to analyze the prevalence and diversity of Synergistetes operational taxonomic units (OTUs) present in plaque samples of both subject groups. RESULTS: A total of 1030 non-chimeric 16S rRNA clones were obtained, of which 162 corresponded to members of the phylum Synergistetes. A significantly larger number of Synergistetes clones were obtained from periodontitis subgingival plaque than from periodontitis-free controls (25.4% vs. 5.9%, p < 0.001). All Synergistetes clones corresponded to cluster A oral Synergistetes, and fell into 31 OTUs (99% sequence identity cut-off). Twenty-nine Synergistetes OTUs were detected in the periodontitis group while eight were detected in the periodontitis-free group (p < 0.001). Five Synergistetes OTUs; including one OTU corresponding to the recently-characterized species Fretibacterium fastidiosum, were more prevalent in the periodontitis subjects (p < 0.05). CONCLUSION: OTUs belonging to oral Synergistetes cluster A were more readily detectable and were more diverse in subgingival plaque from periodontitis subjects compared with periodontitis-free controls. Specific Synergistetes OTUs appear to be associated with periodontitis. SN - 1600-0765 UR - https://www.unboundmedicine.com/medline/citation/22881378/Prevalence_and_diversity_of_Synergistetes_taxa_in_periodontal_health_and_disease_ L2 - https://doi.org/10.1111/j.1600-0765.2012.01516.x DB - PRIME DP - Unbound Medicine ER -