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Defensin like peptide from Panulirus argus relates structurally with beta defensin from vertebrates.
Fish Shellfish Immunol 2012; 33(4):872-9FS

Abstract

Naturally occurring antimicrobial peptides take place in the first line of host defense against pathogen as part of the humoral innate immune response. β-defensins are among the most abundant antimicrobial peptides in mammals, and thought to be solely found in vertebrates until a recent report describing the cloning and sequencing of defensin like peptides in the spiny lobster Panulirus japonicus. In the current study, we cloned and sequenced two genes from the hemocytes of the spiny lobster Panulirus argus encoding for two isoforms of defensin-like peptides, thus confirming the presence of this protein in the Panulirus genus. The 44 amino acids mature peptides showed the conservation of cysteine pattern characterizing the β-defensins, as well as known amino acids residues critical to exert their antimicrobial activity. They are also amphipathics, hydrophobics, and display an overall positive charge (+1) located at the C-terminus. The tertiary structure obtained by homology modeling indicated that likely conformations of lobster peptides are highly similar to β-defensins from vertebrates. The phylogenetic study carried out by probabilistic methods confirmed the relation with ancestral β-defensin from vertebrates. The finding of a putative defensin-like peptide in the expressed sequence tag (EST) of the lobster Homarus americanus with high homology with those of P. argus described in this study, would indicate the presence of this peptides in Palinuridae family. Taking into account all similarities between these peptides with β-defensins from vertebrates, it is conceivable to further support the finding of a new family of β-defensins in invertebrate.

Authors+Show Affiliations

Biochemistry Department, Center for Pharmaceuticals Research and Development, Ave. 26, No. 1605, CP 10 400 Habana, Cuba. vivian.montero@fq.uh.cuNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22885029

Citation

Montero-Alejo, V, et al. "Defensin Like Peptide From Panulirus Argus Relates Structurally With Beta Defensin From Vertebrates." Fish & Shellfish Immunology, vol. 33, no. 4, 2012, pp. 872-9.
Montero-Alejo V, Acosta-Alba J, Perdomo-Morales R, et al. Defensin like peptide from Panulirus argus relates structurally with beta defensin from vertebrates. Fish Shellfish Immunol. 2012;33(4):872-9.
Montero-Alejo, V., Acosta-Alba, J., Perdomo-Morales, R., Perera, E., Hernández-Rodríguez, E. W., Estrada, M. P., & Porto-Verdecia, M. (2012). Defensin like peptide from Panulirus argus relates structurally with beta defensin from vertebrates. Fish & Shellfish Immunology, 33(4), pp. 872-9. doi:10.1016/j.fsi.2012.07.013.
Montero-Alejo V, et al. Defensin Like Peptide From Panulirus Argus Relates Structurally With Beta Defensin From Vertebrates. Fish Shellfish Immunol. 2012;33(4):872-9. PubMed PMID: 22885029.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Defensin like peptide from Panulirus argus relates structurally with beta defensin from vertebrates. AU - Montero-Alejo,V, AU - Acosta-Alba,J, AU - Perdomo-Morales,R, AU - Perera,E, AU - Hernández-Rodríguez,E W, AU - Estrada,M P, AU - Porto-Verdecia,M, Y1 - 2012/08/05/ PY - 2012/03/20/received PY - 2012/07/08/revised PY - 2012/07/27/accepted PY - 2012/8/14/entrez PY - 2012/8/14/pubmed PY - 2013/3/5/medline SP - 872 EP - 9 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 33 IS - 4 N2 - Naturally occurring antimicrobial peptides take place in the first line of host defense against pathogen as part of the humoral innate immune response. β-defensins are among the most abundant antimicrobial peptides in mammals, and thought to be solely found in vertebrates until a recent report describing the cloning and sequencing of defensin like peptides in the spiny lobster Panulirus japonicus. In the current study, we cloned and sequenced two genes from the hemocytes of the spiny lobster Panulirus argus encoding for two isoforms of defensin-like peptides, thus confirming the presence of this protein in the Panulirus genus. The 44 amino acids mature peptides showed the conservation of cysteine pattern characterizing the β-defensins, as well as known amino acids residues critical to exert their antimicrobial activity. They are also amphipathics, hydrophobics, and display an overall positive charge (+1) located at the C-terminus. The tertiary structure obtained by homology modeling indicated that likely conformations of lobster peptides are highly similar to β-defensins from vertebrates. The phylogenetic study carried out by probabilistic methods confirmed the relation with ancestral β-defensin from vertebrates. The finding of a putative defensin-like peptide in the expressed sequence tag (EST) of the lobster Homarus americanus with high homology with those of P. argus described in this study, would indicate the presence of this peptides in Palinuridae family. Taking into account all similarities between these peptides with β-defensins from vertebrates, it is conceivable to further support the finding of a new family of β-defensins in invertebrate. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/22885029/Defensin_like_peptide_from_Panulirus_argus_relates_structurally_with_beta_defensin_from_vertebrates_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(12)00276-8 DB - PRIME DP - Unbound Medicine ER -