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Miniaturized molecularly imprinted matrix solid-phase dispersion coupled with high performance liquid chromatography for rapid determination of auxins in orange samples.
J Chromatogr A. 2012 Sep 21; 1256:1-8.JC

Abstract

A simple, convenient and high selective miniaturized molecularly imprinted matrix solid-phase dispersion (mini-MI-MSPD) technique using l-tryptophan-imprinted polymers as sorbents was proposed for simultaneous extraction of four auxins (indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA)) from orange samples coupled with high performance liquid chromatography-ultraviolet detection. The molecularly imprinted polymers (MIPs) synthesized using l-tryptophan as dummy template showed high affinity and selectivity to auxins, and so they were applied as the specific dispersant of MSPD to simultaneously eliminate the effect of template leakage on quantitative analysis. Under the optimized conditions, good linearity was obtained in a range of 0.02-2.00 μg g(-1) and the average recoveries of four auxins at three spiked levels ranged from 87.6% to 104.5% with the relative standard deviation (RSD) ≤4.4%. The presented mini-MI-MSPD method combined the advantages of MSPD for allowing the extraction, dispersion and homogenization in a single step and the advantages of MIPs for high affinity and selectivity towards four auxins, which could be applied to the determination of auxins in complicated vegetal samples.

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Authors+Show Affiliations

Yan H
College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China. yanhy@hbu.edu.cn
Wang F
No affiliation info available
Wang H
No affiliation info available
Yang G
No affiliation info available

MeSH

AdsorptionChromatography, High Pressure LiquidCitrus sinensisIndoleacetic AcidsKineticsLimit of DetectionMiniaturizationMolecular ImprintingSpectrophotometry, Ultraviolet

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

22885049

Citation

Yan, Hongyuan, et al. "Miniaturized Molecularly Imprinted Matrix Solid-phase Dispersion Coupled With High Performance Liquid Chromatography for Rapid Determination of Auxins in Orange Samples." Journal of Chromatography. A, vol. 1256, 2012, pp. 1-8.
Yan H, Wang F, Wang H, et al. Miniaturized molecularly imprinted matrix solid-phase dispersion coupled with high performance liquid chromatography for rapid determination of auxins in orange samples. J Chromatogr A. 2012;1256:1-8.
Yan, H., Wang, F., Wang, H., & Yang, G. (2012). Miniaturized molecularly imprinted matrix solid-phase dispersion coupled with high performance liquid chromatography for rapid determination of auxins in orange samples. Journal of Chromatography. A, 1256, 1-8. https://doi.org/10.1016/j.chroma.2012.07.037
Yan H, et al. Miniaturized Molecularly Imprinted Matrix Solid-phase Dispersion Coupled With High Performance Liquid Chromatography for Rapid Determination of Auxins in Orange Samples. J Chromatogr A. 2012 Sep 21;1256:1-8. PubMed PMID: 22885049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Miniaturized molecularly imprinted matrix solid-phase dispersion coupled with high performance liquid chromatography for rapid determination of auxins in orange samples. AU - Yan,Hongyuan, AU - Wang,Fang, AU - Wang,Hui, AU - Yang,Gengliang, Y1 - 2012/07/20/ PY - 2012/04/13/received PY - 2012/07/10/revised PY - 2012/07/12/accepted PY - 2012/8/14/entrez PY - 2012/8/14/pubmed PY - 2012/12/10/medline SP - 1 EP - 8 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1256 N2 - A simple, convenient and high selective miniaturized molecularly imprinted matrix solid-phase dispersion (mini-MI-MSPD) technique using l-tryptophan-imprinted polymers as sorbents was proposed for simultaneous extraction of four auxins (indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA)) from orange samples coupled with high performance liquid chromatography-ultraviolet detection. The molecularly imprinted polymers (MIPs) synthesized using l-tryptophan as dummy template showed high affinity and selectivity to auxins, and so they were applied as the specific dispersant of MSPD to simultaneously eliminate the effect of template leakage on quantitative analysis. Under the optimized conditions, good linearity was obtained in a range of 0.02-2.00 μg g(-1) and the average recoveries of four auxins at three spiked levels ranged from 87.6% to 104.5% with the relative standard deviation (RSD) ≤4.4%. The presented mini-MI-MSPD method combined the advantages of MSPD for allowing the extraction, dispersion and homogenization in a single step and the advantages of MIPs for high affinity and selectivity towards four auxins, which could be applied to the determination of auxins in complicated vegetal samples. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/22885049/Miniaturized_molecularly_imprinted_matrix_solid_phase_dispersion_coupled_with_high_performance_liquid_chromatography_for_rapid_determination_of_auxins_in_orange_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(12)01072-2 DB - PRIME DP - Unbound Medicine ER -