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Effects of salinity acclimation on Na(+)/K(+)-ATPase responses and FXYD11 expression in the gills and kidneys of the Japanese eel (Anguilla japonica).
Comp Biochem Physiol A Mol Integr Physiol. 2012 Nov; 163(3-4):302-10.CB

Abstract

Na(+)/K(+)-ATPase (NKA) is a primary active pump provides the driving force for ion-transporting systems in the osmoregulatory tissues of teleosts. Therefore, modulation of NKA expression or activity and its regulatory subunit, FXYD protein, is essential for teleosts in salinity adaptation. To understand the mechanisms for modulation of NKA in catadromous fishes, NKA expression and activity, cloning and mRNA expression of FXYD11 (AjFXYD11) were examined in Japanese eel (Anguilla japonica) exposed to fresh water (FW) and seawater (SW; 35‰). Expression and activity of NKA as well as mRNA expression of AjFXYD11 in gills were elevated in SW eel compared to FW eel. Conversely, NKA responses in eel kidneys were higher in FW group than SW group, whereas no significant difference was found in renal AjFXYD11 expression between the two groups. Comparison of NKA activity and AjFXYD11 expression between two osmoregulatory tissues suggested that AjFXYD11 plays a specific, functional role in gills. However, since cortisol plays an important role for regulation of ion transport in teleost SW acclimation and gill AjFXYD11 expression was elevated in SW eel, the organ culture approach was used to study the effect of cortisol on gill AjFXYD11 mRNA expression. Our results revealed that cortisol treatment increased the levels of gill AjFXYD11 transcripts. This finding suggested that cortisol could be involved in the regulation of NKA by altering AjFXYD11 expression during the process of SW acclimation in A. japonica. Taken together, the differential expression of branchial and renal NKA and AjFXYD11 implicated their roles in the osmotic homeostasis of Japanese eel exposed to environments of different salinities.

Authors+Show Affiliations

Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22885345

Citation

Tang, Cheng-Hao, et al. "Effects of Salinity Acclimation On Na(+)/K(+)-ATPase Responses and FXYD11 Expression in the Gills and Kidneys of the Japanese Eel (Anguilla Japonica)." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 163, no. 3-4, 2012, pp. 302-10.
Tang CH, Lai DY, Lee TH. Effects of salinity acclimation on Na(+)/K(+)-ATPase responses and FXYD11 expression in the gills and kidneys of the Japanese eel (Anguilla japonica). Comp Biochem Physiol A Mol Integr Physiol. 2012;163(3-4):302-10.
Tang, C. H., Lai, D. Y., & Lee, T. H. (2012). Effects of salinity acclimation on Na(+)/K(+)-ATPase responses and FXYD11 expression in the gills and kidneys of the Japanese eel (Anguilla japonica). Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 163(3-4), 302-10. https://doi.org/10.1016/j.cbpa.2012.07.017
Tang CH, Lai DY, Lee TH. Effects of Salinity Acclimation On Na(+)/K(+)-ATPase Responses and FXYD11 Expression in the Gills and Kidneys of the Japanese Eel (Anguilla Japonica). Comp Biochem Physiol A Mol Integr Physiol. 2012;163(3-4):302-10. PubMed PMID: 22885345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of salinity acclimation on Na(+)/K(+)-ATPase responses and FXYD11 expression in the gills and kidneys of the Japanese eel (Anguilla japonica). AU - Tang,Cheng-Hao, AU - Lai,Dong-Yang, AU - Lee,Tsung-Han, Y1 - 2012/08/03/ PY - 2012/05/08/received PY - 2012/07/25/revised PY - 2012/07/30/accepted PY - 2012/8/14/entrez PY - 2012/8/14/pubmed PY - 2013/1/29/medline SP - 302 EP - 10 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 163 IS - 3-4 N2 - Na(+)/K(+)-ATPase (NKA) is a primary active pump provides the driving force for ion-transporting systems in the osmoregulatory tissues of teleosts. Therefore, modulation of NKA expression or activity and its regulatory subunit, FXYD protein, is essential for teleosts in salinity adaptation. To understand the mechanisms for modulation of NKA in catadromous fishes, NKA expression and activity, cloning and mRNA expression of FXYD11 (AjFXYD11) were examined in Japanese eel (Anguilla japonica) exposed to fresh water (FW) and seawater (SW; 35‰). Expression and activity of NKA as well as mRNA expression of AjFXYD11 in gills were elevated in SW eel compared to FW eel. Conversely, NKA responses in eel kidneys were higher in FW group than SW group, whereas no significant difference was found in renal AjFXYD11 expression between the two groups. Comparison of NKA activity and AjFXYD11 expression between two osmoregulatory tissues suggested that AjFXYD11 plays a specific, functional role in gills. However, since cortisol plays an important role for regulation of ion transport in teleost SW acclimation and gill AjFXYD11 expression was elevated in SW eel, the organ culture approach was used to study the effect of cortisol on gill AjFXYD11 mRNA expression. Our results revealed that cortisol treatment increased the levels of gill AjFXYD11 transcripts. This finding suggested that cortisol could be involved in the regulation of NKA by altering AjFXYD11 expression during the process of SW acclimation in A. japonica. Taken together, the differential expression of branchial and renal NKA and AjFXYD11 implicated their roles in the osmotic homeostasis of Japanese eel exposed to environments of different salinities. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/22885345/Effects_of_salinity_acclimation_on_Na_+_/K_+__ATPase_responses_and_FXYD11_expression_in_the_gills_and_kidneys_of_the_Japanese_eel__Anguilla_japonica__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(12)00438-2 DB - PRIME DP - Unbound Medicine ER -