Tags

Type your tag names separated by a space and hit enter

Isoflurane-loaded nanoemulsion prepared by high-pressure homogenization: investigation of stability and dose reduction in general anesthesia.
J Biomed Nanotechnol. 2012 Oct; 8(5):849-58.JB

Abstract

Isoflurane is a halogenated ether which is used for general anesthesia. To stabilize a new formulation in order to evaluate the potential to reduce the dose required for general anesthesia, an isoflurane-loaded nanoemulsion was proposed. A high-pressure homogenization technique was used to develop drug-loaded nanoemulsions which presented droplet size of 150 +/- 0.78 nm with a narrow size distribution and low polydispersity index (0.08 +/- 0.01). The zeta potential was -18 +/- 2.4 mV and pH was 6.03 +/- 0.04. Rheological analysis showed Newtonian behavior for the formulations, whose physical stability was confirmed by multiple light scattering. It was verified that isoflurane volatilization did not occur in these formulations. The preclinical evaluation, carried out via the end-tidal isoflurane concentration, showed that the dose required for anesthetic maintenance significantly decreased when the nanostructured formulation was administered compared to inhaled isoflurane. There was no significant difference (p < 0.05) between experimental groups (inhaled isoflurane and intravenous isoflurane-loaded nanoemulsion) in terms of the cardiac rate, oxygen hemoglobin saturation, and arterial blood pressure, as well as the biomarkers of renal, hepatic and skeletal muscle system functionalities. Slight tachypnea, edema, and erythema were observed after isoflurane-loaded or unloaded-nanoemulsion. The stability and significant dose reduction observed for drug-loaded nanoemulsion render this formulation a promising option for intravenous delivery of isoflurane.

Authors+Show Affiliations

Programa de Pós-Graduação em Ciências Farmacéuticas, Faculdade de Farmàcia, Universidade Federal do Rio Grande do Sul, Porto Alegre, 90040-060, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22888757

Citation

Krahn, Carolina L., et al. "Isoflurane-loaded Nanoemulsion Prepared By High-pressure Homogenization: Investigation of Stability and Dose Reduction in General Anesthesia." Journal of Biomedical Nanotechnology, vol. 8, no. 5, 2012, pp. 849-58.
Krahn CL, Raffin RP, Santos GS, et al. Isoflurane-loaded nanoemulsion prepared by high-pressure homogenization: investigation of stability and dose reduction in general anesthesia. J Biomed Nanotechnol. 2012;8(5):849-58.
Krahn, C. L., Raffin, R. P., Santos, G. S., Queiroga, L. B., Cavalcanti, R. L., Serpa, P., Dallegrave, E., Mayorga, P. E., Pohlmann, A. R., Natalini, C. C., Guterres, S. S., & Limberger, R. P. (2012). Isoflurane-loaded nanoemulsion prepared by high-pressure homogenization: investigation of stability and dose reduction in general anesthesia. Journal of Biomedical Nanotechnology, 8(5), 849-58.
Krahn CL, et al. Isoflurane-loaded Nanoemulsion Prepared By High-pressure Homogenization: Investigation of Stability and Dose Reduction in General Anesthesia. J Biomed Nanotechnol. 2012;8(5):849-58. PubMed PMID: 22888757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isoflurane-loaded nanoemulsion prepared by high-pressure homogenization: investigation of stability and dose reduction in general anesthesia. AU - Krahn,Carolina L, AU - Raffin,Renata P, AU - Santos,Guilherme S, AU - Queiroga,Luciana B, AU - Cavalcanti,Ruben L, AU - Serpa,Priscila, AU - Dallegrave,Eliane, AU - Mayorga,Paulo E, AU - Pohlmann,Adriana R, AU - Natalini,Cláudio C, AU - Guterres,Silvia S, AU - Limberger,Renata P, PY - 2012/8/15/entrez PY - 2012/8/15/pubmed PY - 2012/9/7/medline SP - 849 EP - 58 JF - Journal of biomedical nanotechnology JO - J Biomed Nanotechnol VL - 8 IS - 5 N2 - Isoflurane is a halogenated ether which is used for general anesthesia. To stabilize a new formulation in order to evaluate the potential to reduce the dose required for general anesthesia, an isoflurane-loaded nanoemulsion was proposed. A high-pressure homogenization technique was used to develop drug-loaded nanoemulsions which presented droplet size of 150 +/- 0.78 nm with a narrow size distribution and low polydispersity index (0.08 +/- 0.01). The zeta potential was -18 +/- 2.4 mV and pH was 6.03 +/- 0.04. Rheological analysis showed Newtonian behavior for the formulations, whose physical stability was confirmed by multiple light scattering. It was verified that isoflurane volatilization did not occur in these formulations. The preclinical evaluation, carried out via the end-tidal isoflurane concentration, showed that the dose required for anesthetic maintenance significantly decreased when the nanostructured formulation was administered compared to inhaled isoflurane. There was no significant difference (p < 0.05) between experimental groups (inhaled isoflurane and intravenous isoflurane-loaded nanoemulsion) in terms of the cardiac rate, oxygen hemoglobin saturation, and arterial blood pressure, as well as the biomarkers of renal, hepatic and skeletal muscle system functionalities. Slight tachypnea, edema, and erythema were observed after isoflurane-loaded or unloaded-nanoemulsion. The stability and significant dose reduction observed for drug-loaded nanoemulsion render this formulation a promising option for intravenous delivery of isoflurane. SN - 1550-7033 UR - https://www.unboundmedicine.com/medline/citation/22888757/Isoflurane_loaded_nanoemulsion_prepared_by_high_pressure_homogenization:_investigation_of_stability_and_dose_reduction_in_general_anesthesia_ L2 - https://www.lens.org/lens/search?q=citation_id:22888757 DB - PRIME DP - Unbound Medicine ER -