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Genetic map of Triticum turgidum based on a hexaploid wheat population without genetic recombination for D genome.
BMC Genet. 2012 Aug 13; 13:69.BG

Abstract

BACKGROUND

A synthetic doubled-haploid hexaploid wheat population, SynDH1, derived from the spontaneous chromosome doubling of triploid F1 hybrid plants obtained from the cross of hybrids Triticum turgidum ssp. durum line Langdon (LDN) and ssp. turgidum line AS313, with Aegilops tauschii ssp. tauschii accession AS60, was previously constructed. SynDH1 is a tetraploidization-hexaploid doubled haploid (DH) population because it contains recombinant A and B chromosomes from two different T. turgidum genotypes, while all the D chromosomes from Ae. tauschii are homogenous across the whole population. This paper reports the construction of a genetic map using this population.

RESULTS

Of the 606 markers used to assemble the genetic map, 588 (97%) were assigned to linkage groups. These included 513 Diversity Arrays Technology (DArT) markers, 72 simple sequence repeat (SSR), one insertion site-based polymorphism (ISBP), and two high-molecular-weight glutenin subunit (HMW-GS) markers. These markers were assigned to the 14 chromosomes, covering 2048.79 cM, with a mean distance of 3.48 cM between adjacent markers. This map showed good coverage of the A and B genome chromosomes, apart from 3A, 5A, 6A, and 4B. Compared with previously reported maps, most shared markers showed highly consistent orders. This map was successfully used to identify five quantitative trait loci (QTL), including two for spikelet number on chromosomes 7A and 5B, two for spike length on 7A and 3B, and one for 1000-grain weight on 4B. However, differences in crossability QTL between the two T. turgidum parents may explain the segregation distortion regions on chromosomes 1A, 3B, and 6B.

CONCLUSIONS

A genetic map of T. turgidum including 588 markers was constructed using a synthetic doubled haploid (SynDH) hexaploid wheat population. Five QTLs for three agronomic traits were identified from this population. However, more markers are needed to increase the density and resolution of this map in the future study.

Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22888829

Citation

Zhang, Li, et al. "Genetic Map of Triticum Turgidum Based On a Hexaploid Wheat Population Without Genetic Recombination for D Genome." BMC Genetics, vol. 13, 2012, p. 69.
Zhang L, Luo JT, Hao M, et al. Genetic map of Triticum turgidum based on a hexaploid wheat population without genetic recombination for D genome. BMC Genet. 2012;13:69.
Zhang, L., Luo, J. T., Hao, M., Zhang, L. Q., Yuan, Z. W., Yan, Z. H., Liu, Y. X., Zhang, B., Liu, B. L., Liu, C. J., Zhang, H. G., Zheng, Y. L., & Liu, D. C. (2012). Genetic map of Triticum turgidum based on a hexaploid wheat population without genetic recombination for D genome. BMC Genetics, 13, 69. https://doi.org/10.1186/1471-2156-13-69
Zhang L, et al. Genetic Map of Triticum Turgidum Based On a Hexaploid Wheat Population Without Genetic Recombination for D Genome. BMC Genet. 2012 Aug 13;13:69. PubMed PMID: 22888829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic map of Triticum turgidum based on a hexaploid wheat population without genetic recombination for D genome. AU - Zhang,Li, AU - Luo,Jiang-Tao, AU - Hao,Ming, AU - Zhang,Lian-Quan, AU - Yuan,Zhong-Wei, AU - Yan,Ze-Hong, AU - Liu,Ya-Xi, AU - Zhang,Bo, AU - Liu,Bao-Long, AU - Liu,Chun-Ji, AU - Zhang,Huai-Gang, AU - Zheng,You-Liang, AU - Liu,Deng-Cai, Y1 - 2012/08/13/ PY - 2012/02/20/received PY - 2012/08/08/accepted PY - 2012/8/15/entrez PY - 2012/8/15/pubmed PY - 2013/2/21/medline SP - 69 EP - 69 JF - BMC genetics JO - BMC Genet VL - 13 N2 - BACKGROUND: A synthetic doubled-haploid hexaploid wheat population, SynDH1, derived from the spontaneous chromosome doubling of triploid F1 hybrid plants obtained from the cross of hybrids Triticum turgidum ssp. durum line Langdon (LDN) and ssp. turgidum line AS313, with Aegilops tauschii ssp. tauschii accession AS60, was previously constructed. SynDH1 is a tetraploidization-hexaploid doubled haploid (DH) population because it contains recombinant A and B chromosomes from two different T. turgidum genotypes, while all the D chromosomes from Ae. tauschii are homogenous across the whole population. This paper reports the construction of a genetic map using this population. RESULTS: Of the 606 markers used to assemble the genetic map, 588 (97%) were assigned to linkage groups. These included 513 Diversity Arrays Technology (DArT) markers, 72 simple sequence repeat (SSR), one insertion site-based polymorphism (ISBP), and two high-molecular-weight glutenin subunit (HMW-GS) markers. These markers were assigned to the 14 chromosomes, covering 2048.79 cM, with a mean distance of 3.48 cM between adjacent markers. This map showed good coverage of the A and B genome chromosomes, apart from 3A, 5A, 6A, and 4B. Compared with previously reported maps, most shared markers showed highly consistent orders. This map was successfully used to identify five quantitative trait loci (QTL), including two for spikelet number on chromosomes 7A and 5B, two for spike length on 7A and 3B, and one for 1000-grain weight on 4B. However, differences in crossability QTL between the two T. turgidum parents may explain the segregation distortion regions on chromosomes 1A, 3B, and 6B. CONCLUSIONS: A genetic map of T. turgidum including 588 markers was constructed using a synthetic doubled haploid (SynDH) hexaploid wheat population. Five QTLs for three agronomic traits were identified from this population. However, more markers are needed to increase the density and resolution of this map in the future study. SN - 1471-2156 UR - https://www.unboundmedicine.com/medline/citation/22888829/Genetic_map_of_Triticum_turgidum_based_on_a_hexaploid_wheat_population_without_genetic_recombination_for_D_genome_ L2 - https://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-13-69 DB - PRIME DP - Unbound Medicine ER -