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Buckwheat (Fagopyrum esculentum Moench) grain and fractions: antioxidant compounds and activities.
J Food Sci. 2012 Sep; 77(9):C954-9.JF

Abstract

Buckwheat (Fagopyrum esculentum Moench) is an alternative crop belonging to the Polygonaceae family. In comparison to antioxidant activity of frequently used cereals, buckwheat has been reported to possess higher antioxidant activity (AOA), mainly due to high rutin content. The objective of this work was to determine the main antioxidant compounds and AOA of buckwheat grain fractions (whole grain, hull, and groat). Buckwheat grain fractions were extracted with ethanol/water (80/20, v/v), followed by determination of total phenolic and flavonoid content. Quantification of phenolic compounds and tocopherols was performed by high-performance liquid chromatography (HPLC). The AOA was estimated by 2 direct electron spin resonance (ESR) and 4 indirect (spectrophotometric) tests. Significantly higher contents of total phenolics and total flavonoids were found in buckwheat hull than in whole grain and groat. Protocatechuic, syringic, and sinapic acid, rutin, and quercetin were found in all tested fractions, whereas vanilic acid was found in whole grain and hull. The content of total tocopherols in investigated samples ranged from 23.3 mmol/g for hull to 61.8 mmol/g for groat. Hull was superior in scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), hydroxyl ((•)OH), and superoxide anion (O(2)(•-)) radicals, reducing activity, AOA by β-carotene bleaching method, and chelating activity on Fe(2+) as evidenced by its lower IC(50) value. Obtained results can broaden the utilization of buckwheat, especially a share of hull in whole grain flour production.

PRACTICAL APPLICATION

Obtained results suggest possibility to supplement the whole grain buckwheat flour with hull, which leads toward better usage of by-products in buckwheat production, and enhancement of antioxidant potential of the final product.

Authors+Show Affiliations

Inst of Food Technology in Novi Sad, Univ of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia. ivana.sedej@fins.uns.ac.rsNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22888949

Citation

Sedej, Ivana, et al. "Buckwheat (Fagopyrum Esculentum Moench) Grain and Fractions: Antioxidant Compounds and Activities." Journal of Food Science, vol. 77, no. 9, 2012, pp. C954-9.
Sedej I, Sakač M, Mandić A, et al. Buckwheat (Fagopyrum esculentum Moench) grain and fractions: antioxidant compounds and activities. J Food Sci. 2012;77(9):C954-9.
Sedej, I., Sakač, M., Mandić, A., Mišan, A., Tumbas, V., & Čanadanović-Brunet, J. (2012). Buckwheat (Fagopyrum esculentum Moench) grain and fractions: antioxidant compounds and activities. Journal of Food Science, 77(9), C954-9. https://doi.org/10.1111/j.1750-3841.2012.02867.x
Sedej I, et al. Buckwheat (Fagopyrum Esculentum Moench) Grain and Fractions: Antioxidant Compounds and Activities. J Food Sci. 2012;77(9):C954-9. PubMed PMID: 22888949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Buckwheat (Fagopyrum esculentum Moench) grain and fractions: antioxidant compounds and activities. AU - Sedej,Ivana, AU - Sakač,Marijana, AU - Mandić,Anamarija, AU - Mišan,Aleksandra, AU - Tumbas,Vesna, AU - Čanadanović-Brunet,Jasna, Y1 - 2012/08/13/ PY - 2012/8/15/entrez PY - 2012/8/15/pubmed PY - 2013/2/1/medline SP - C954 EP - 9 JF - Journal of food science JO - J Food Sci VL - 77 IS - 9 N2 - UNLABELLED: Buckwheat (Fagopyrum esculentum Moench) is an alternative crop belonging to the Polygonaceae family. In comparison to antioxidant activity of frequently used cereals, buckwheat has been reported to possess higher antioxidant activity (AOA), mainly due to high rutin content. The objective of this work was to determine the main antioxidant compounds and AOA of buckwheat grain fractions (whole grain, hull, and groat). Buckwheat grain fractions were extracted with ethanol/water (80/20, v/v), followed by determination of total phenolic and flavonoid content. Quantification of phenolic compounds and tocopherols was performed by high-performance liquid chromatography (HPLC). The AOA was estimated by 2 direct electron spin resonance (ESR) and 4 indirect (spectrophotometric) tests. Significantly higher contents of total phenolics and total flavonoids were found in buckwheat hull than in whole grain and groat. Protocatechuic, syringic, and sinapic acid, rutin, and quercetin were found in all tested fractions, whereas vanilic acid was found in whole grain and hull. The content of total tocopherols in investigated samples ranged from 23.3 mmol/g for hull to 61.8 mmol/g for groat. Hull was superior in scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), hydroxyl ((•)OH), and superoxide anion (O(2)(•-)) radicals, reducing activity, AOA by β-carotene bleaching method, and chelating activity on Fe(2+) as evidenced by its lower IC(50) value. Obtained results can broaden the utilization of buckwheat, especially a share of hull in whole grain flour production. PRACTICAL APPLICATION: Obtained results suggest possibility to supplement the whole grain buckwheat flour with hull, which leads toward better usage of by-products in buckwheat production, and enhancement of antioxidant potential of the final product. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/22888949/Buckwheat__Fagopyrum_esculentum_Moench__grain_and_fractions:_antioxidant_compounds_and_activities_ L2 - https://doi.org/10.1111/j.1750-3841.2012.02867.x DB - PRIME DP - Unbound Medicine ER -