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Biological activity assessment and phenolic compounds characterization from the fruit pericarp of Litchi chinensis for cosmetic applications.
Pharm Biol. 2012 Nov; 50(11):1384-90.PB

Abstract

CONTEXT

Litchi chinensis Sonn. (Spindaceae) is an important economic fruit of Thailand. Therapeutic effects of the fruits are contributed by anti-inflammatory phenolics.

OBJECTIVE

To extract the litchi fruit pericarp in order to identify biologically actives substances with potential for cosmetic application.

MATERIALS AND METHODS

The litchi pericarp was macerated by 70% ethanol (EtOH) and partitioned using n-hexane and ethyl acetate (EtOAc). In vitro antioxidant activities were assessed by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), ABTS and ferric reducing ability of plasma (FRAP) assays including tyrosinase inhibitory effect. Cellular radical scavenging capacity was monitored in a normal human fibroblast cell culture (NHF). Total phenolic content was determined and characterized by HPLC.

RESULTS

The EtOAc fraction was a significant antioxidant, stronger than ascorbic acid (p < 0.01), as assessed by ABTS (IC(50) = 7.137 ± 0.021 μg/mL), DPPH (IC(50) = 2.288 ± 0.063 μg/mL) and FRAP (EC(1mMFeSO4) = 8013.183 ± 58.804 μg/mL) assays. It demonstrated an antityrosinase effect (IC(50) = 197.860 ± 1.230 μg/mL) and showed no cytotoxic activity toward Vero and NHF cells, at a maximum tested concentration (50 μg/mL), with cellular antioxidant activity. Total phenolic content was highest in the most potent antioxidant fraction. Quercetin, rosmarinic and gallic acids were found. Total phenolic content is highly related to FRAP, antityrosinase, and ABTS activities.

DISCUSSION AND CONCLUSION

Pericarp from litchi fruit can be obtained abundantly from agricultural waste, and the strong antioxidant activity demonstrated in this report may have application in topical cosmetic products. This ecological antioxidant can be prepared using a feasible method resulting in less waste and increased agro-industrial profitability.

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Publisher Full Text

Authors+Show Affiliations

Kanlayavattanakul M
School of Cosmetic Science, Mae Fah Luang University, Chiang Rai, Thailand. mayuree@mfu.ac.th
Ospondpant D
No affiliation info available
Ruktanonchai U
No affiliation info available
Lourith N
No affiliation info available

MeSH

AnimalsAntioxidantsAscorbic AcidCells, CulturedChlorocebus aethiopsChromatography, High Pressure LiquidCosmeticsDose-Response Relationship, DrugFeasibility StudiesFibroblastsFree Radical ScavengersFruitHumansInhibitory Concentration 50LitchiMonophenol MonooxygenasePhenolsPlant ExtractsThailandVero Cells

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22889092

Citation

Kanlayavattanakul, Mayuree, et al. "Biological Activity Assessment and Phenolic Compounds Characterization From the Fruit Pericarp of Litchi Chinensis for Cosmetic Applications." Pharmaceutical Biology, vol. 50, no. 11, 2012, pp. 1384-90.
Kanlayavattanakul M, Ospondpant D, Ruktanonchai U, et al. Biological activity assessment and phenolic compounds characterization from the fruit pericarp of Litchi chinensis for cosmetic applications. Pharm Biol. 2012;50(11):1384-90.
Kanlayavattanakul, M., Ospondpant, D., Ruktanonchai, U., & Lourith, N. (2012). Biological activity assessment and phenolic compounds characterization from the fruit pericarp of Litchi chinensis for cosmetic applications. Pharmaceutical Biology, 50(11), 1384-90. https://doi.org/10.3109/13880209.2012.675342
Kanlayavattanakul M, et al. Biological Activity Assessment and Phenolic Compounds Characterization From the Fruit Pericarp of Litchi Chinensis for Cosmetic Applications. Pharm Biol. 2012;50(11):1384-90. PubMed PMID: 22889092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biological activity assessment and phenolic compounds characterization from the fruit pericarp of Litchi chinensis for cosmetic applications. AU - Kanlayavattanakul,Mayuree, AU - Ospondpant,Dusadee, AU - Ruktanonchai,Uracha, AU - Lourith,Nattaya, Y1 - 2012/09/11/ PY - 2012/8/15/entrez PY - 2012/8/15/pubmed PY - 2013/3/2/medline SP - 1384 EP - 90 JF - Pharmaceutical biology JO - Pharm Biol VL - 50 IS - 11 N2 - CONTEXT: Litchi chinensis Sonn. (Spindaceae) is an important economic fruit of Thailand. Therapeutic effects of the fruits are contributed by anti-inflammatory phenolics. OBJECTIVE: To extract the litchi fruit pericarp in order to identify biologically actives substances with potential for cosmetic application. MATERIALS AND METHODS: The litchi pericarp was macerated by 70% ethanol (EtOH) and partitioned using n-hexane and ethyl acetate (EtOAc). In vitro antioxidant activities were assessed by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), ABTS and ferric reducing ability of plasma (FRAP) assays including tyrosinase inhibitory effect. Cellular radical scavenging capacity was monitored in a normal human fibroblast cell culture (NHF). Total phenolic content was determined and characterized by HPLC. RESULTS: The EtOAc fraction was a significant antioxidant, stronger than ascorbic acid (p < 0.01), as assessed by ABTS (IC(50) = 7.137 ± 0.021 μg/mL), DPPH (IC(50) = 2.288 ± 0.063 μg/mL) and FRAP (EC(1mMFeSO4) = 8013.183 ± 58.804 μg/mL) assays. It demonstrated an antityrosinase effect (IC(50) = 197.860 ± 1.230 μg/mL) and showed no cytotoxic activity toward Vero and NHF cells, at a maximum tested concentration (50 μg/mL), with cellular antioxidant activity. Total phenolic content was highest in the most potent antioxidant fraction. Quercetin, rosmarinic and gallic acids were found. Total phenolic content is highly related to FRAP, antityrosinase, and ABTS activities. DISCUSSION AND CONCLUSION: Pericarp from litchi fruit can be obtained abundantly from agricultural waste, and the strong antioxidant activity demonstrated in this report may have application in topical cosmetic products. This ecological antioxidant can be prepared using a feasible method resulting in less waste and increased agro-industrial profitability. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/22889092/Biological_activity_assessment_and_phenolic_compounds_characterization_from_the_fruit_pericarp_of_Litchi_chinensis_for_cosmetic_applications_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2012.675342 DB - PRIME DP - Unbound Medicine ER -