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An Fe3O4-nanoparticles-based amperometric biosensor for creatine determination.
Artif Cells Nanomed Biotechnol. 2013 Feb; 41(1):2-7.AC

Abstract

An amperometric biosensor for the detection of creatine was designed, based on carbon paste electrode modified with Fe(3)O(4) nanoparticles. Electron transfer properties of unmodified and Fe(3)O(4)-nanoparticles-modified carbon paste electrodes were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods. Fe(3)O(4) nanoparticles increased the surface area and electric conductivity of the electrode, thus enhancing the sensitivity of the electrode. Optimum pH, buffer concentration, working potential and enzyme loading were selected as 7.0; 0.05 mol L(-1); +0.30 V and 2.0 Unit creatinase (CI), 1.0 Unit sarcosine oxidase (SO), respectively. The purposed biosensor exhibited linear response from 2.0 × 10(-7) mol L(-1) to 3.8 × 10(-6) mol L(-1) and from 9.0 × 10(-6) mol L(-1) to 1.2 × 10(-4) mol L(-1) with a detection limit of 2.0 × 10(-7) mol L(-1). Biosensor was used for determination of creatine in commercial creatine powder samples and showed a good sensing performance.

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Authors+Show Affiliations

Kaçar C
Faculty of Science, Department of Chemistry, Ankara University, Tandoğan, Ankara, Turkey.
Erden PE
No affiliation info available
Pekyardimci S
No affiliation info available
Kiliç E
No affiliation info available

MeSH

Biosensing TechniquesCarbonCreatineDielectric SpectroscopyEnzymes, ImmobilizedFerrosoferric OxideHumansHydrogen-Ion ConcentrationMetal NanoparticlesSarcosine OxidaseSensitivity and SpecificityUreohydrolases

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22889270

Citation

Kaçar, Ceren, et al. "An Fe3O4-nanoparticles-based Amperometric Biosensor for Creatine Determination." Artificial Cells, Nanomedicine, and Biotechnology, vol. 41, no. 1, 2013, pp. 2-7.
Kaçar C, Erden PE, Pekyardimci S, et al. An Fe3O4-nanoparticles-based amperometric biosensor for creatine determination. Artif Cells Nanomed Biotechnol. 2013;41(1):2-7.
Kaçar, C., Erden, P. E., Pekyardimci, S., & Kiliç, E. (2013). An Fe3O4-nanoparticles-based amperometric biosensor for creatine determination. Artificial Cells, Nanomedicine, and Biotechnology, 41(1), 2-7. https://doi.org/10.3109/10731199.2012.712044
Kaçar C, et al. An Fe3O4-nanoparticles-based Amperometric Biosensor for Creatine Determination. Artif Cells Nanomed Biotechnol. 2013;41(1):2-7. PubMed PMID: 22889270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An Fe3O4-nanoparticles-based amperometric biosensor for creatine determination. AU - Kaçar,Ceren, AU - Erden,Pinar Esra, AU - Pekyardimci,Sule, AU - Kiliç,Esma, Y1 - 2012/08/14/ PY - 2012/8/15/entrez PY - 2012/8/15/pubmed PY - 2013/7/24/medline SP - 2 EP - 7 JF - Artificial cells, nanomedicine, and biotechnology JO - Artif Cells Nanomed Biotechnol VL - 41 IS - 1 N2 - An amperometric biosensor for the detection of creatine was designed, based on carbon paste electrode modified with Fe(3)O(4) nanoparticles. Electron transfer properties of unmodified and Fe(3)O(4)-nanoparticles-modified carbon paste electrodes were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods. Fe(3)O(4) nanoparticles increased the surface area and electric conductivity of the electrode, thus enhancing the sensitivity of the electrode. Optimum pH, buffer concentration, working potential and enzyme loading were selected as 7.0; 0.05 mol L(-1); +0.30 V and 2.0 Unit creatinase (CI), 1.0 Unit sarcosine oxidase (SO), respectively. The purposed biosensor exhibited linear response from 2.0 × 10(-7) mol L(-1) to 3.8 × 10(-6) mol L(-1) and from 9.0 × 10(-6) mol L(-1) to 1.2 × 10(-4) mol L(-1) with a detection limit of 2.0 × 10(-7) mol L(-1). Biosensor was used for determination of creatine in commercial creatine powder samples and showed a good sensing performance. SN - 2169-141X UR - https://www.unboundmedicine.com/medline/citation/22889270/An_Fe3O4_nanoparticles_based_amperometric_biosensor_for_creatine_determination_ L2 - https://www.tandfonline.com/doi/full/10.3109/10731199.2012.712044 DB - PRIME DP - Unbound Medicine ER -