Prime

Type your tag names separated by a space and hit enter

Evaluation of antitumor activity of Mimusops elengi leaves on Ehrlich's ascites carcinoma-treated mice.

Abstract

CONTEXT

Mimusops elengi (M. elengi) Linn. (Sapotaceae) has been used as a folk medicine in wound healing, and the treatment of pain, and inflammation in many parts of India.

OBJECTIVE

The purpose of this investigation was to explore the antitumor activity of methanol extract of M. elengi (MEME) in Swiss albino mice against Ehrlich ascites carcinoma (EAC) cell line.

MATERIALS AND METHODS

Twenty-four hours after intraperitoneal (i.p.) inoculation of tumor (EAC) cells in mice (n = 12), MEME was administered at 200 and 300 mg/kg body weight daily for 9 consecutive days. On day 10, half of the mice were dissected and the rest were kept alive for assessment of increase in life span. The antitumor effect of MEME was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameter, and biochemical estimations. In vivo antioxidant parameters were assayed by estimating liver tissue enzyme. In vitro cytotoxicity assay of MEME was measured by using trypan blue exclusion method.

RESULTS AND DISCUSSION

MEME showed significant (p < .001) decrease in tumor volume, packed cell volume, and viable cell count, and increased the life span of EAC bearing mice. Hematological, biochemical profile, and in vivo antioxidant parameters were significantly restored toward normal levels in MEME-treated mice as compared to EAC control. MEME also showed direct cytotoxicity on EAC cell line in a dose-dependent manner.

CONCLUSIONS

The present study demonstrates that M. elengi leaves exhibited antitumor activity in Swiss mice, which may be due to its cytotoxic effect and antioxidant properties.

Links

  • Aggregator Full Text
  • Authors+Show Affiliations

    ,

    Division of Pharmacology, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.

    , , , ,

    Source

    Journal of dietary supplements 9:3 2012 Sep pg 166-77

    MeSH

    Animals
    Antineoplastic Agents, Phytogenic
    Antioxidants
    Ascites
    Carcinoma, Ehrlich Tumor
    Male
    Mice
    Mice, Inbred Strains
    Mimusops
    Phytotherapy
    Plant Extracts
    Plant Leaves

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22891989

    Citation

    TY - JOUR T1 - Evaluation of antitumor activity of Mimusops elengi leaves on Ehrlich's ascites carcinoma-treated mice. AU - Kar,Biswakanth, AU - Kumar,R B Suresh, AU - Bala,Asis, AU - Dolai,Narayan, AU - Mazumder,Upal Kanti, AU - Haldar,Pallab Kanti, PY - 2012/8/16/entrez PY - 2012/8/16/pubmed PY - 2012/12/18/medline SP - 166 EP - 77 JF - Journal of dietary supplements JO - J Diet Suppl VL - 9 IS - 3 N2 - CONTEXT: Mimusops elengi (M. elengi) Linn. (Sapotaceae) has been used as a folk medicine in wound healing, and the treatment of pain, and inflammation in many parts of India. OBJECTIVE: The purpose of this investigation was to explore the antitumor activity of methanol extract of M. elengi (MEME) in Swiss albino mice against Ehrlich ascites carcinoma (EAC) cell line. MATERIALS AND METHODS: Twenty-four hours after intraperitoneal (i.p.) inoculation of tumor (EAC) cells in mice (n = 12), MEME was administered at 200 and 300 mg/kg body weight daily for 9 consecutive days. On day 10, half of the mice were dissected and the rest were kept alive for assessment of increase in life span. The antitumor effect of MEME was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameter, and biochemical estimations. In vivo antioxidant parameters were assayed by estimating liver tissue enzyme. In vitro cytotoxicity assay of MEME was measured by using trypan blue exclusion method. RESULTS AND DISCUSSION: MEME showed significant (p < .001) decrease in tumor volume, packed cell volume, and viable cell count, and increased the life span of EAC bearing mice. Hematological, biochemical profile, and in vivo antioxidant parameters were significantly restored toward normal levels in MEME-treated mice as compared to EAC control. MEME also showed direct cytotoxicity on EAC cell line in a dose-dependent manner. CONCLUSIONS: The present study demonstrates that M. elengi leaves exhibited antitumor activity in Swiss mice, which may be due to its cytotoxic effect and antioxidant properties. SN - 1939-022X UR - https://www.unboundmedicine.com/medline/citation/22891989/Evaluation_of_antitumor_activity_of_Mimusops_elengi_leaves_on_Ehrlich's_ascites_carcinoma_treated_mice_ L2 - http://openurl.ebscohost.com/linksvc/linking.aspx?genre=article&amp;sid=PubMed&amp;issn=1939-0211&amp;title=J Diet Suppl&amp;volume=9&amp;issue=3&amp;spage=166&amp;atitle=Evaluation of antitumor activity of Mimusops elengi leaves on Ehrlich's ascites carcinoma-treated mice.&amp;aulast=Kar&amp;date=2012 ER -