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The same oleosin isoforms are present in oil bodies of rice embryo and aleurone layer while caleosin exists only in those of the embryo.
Plant Physiol Biochem. 2012 Nov; 60:18-24.PP

Abstract

Oil bodies of similar sizes were observed in the cells of embryo and aleurone layer of rice seeds, and remained their structural integrity in vitro after isolation. Comparably, two abundant oleosin isoforms were found in both preparations of oil bodies isolated from the embryo and the aleurone layer. Immunological detection and mass spectrometric analyses indicated that the two oleosin isoforms, termed oleosin-H and oleosin-L, in the embryo and those in the aleurone layer were identical proteins encoded by the same genes (BAF12898.1 and BAF15387.1 for oleosin-H and oleosin-L, respectively). In contrast, one caleosin was found in oil bodies isolated from the embryo but not those isolated from the aleurone layer. Immunological staining of rice seeds confirms that oleosin is present in both embryo and aleurone layer while caleosin exists only in embryo. Caleosin extracted from oil bodies of rice embryo migrated faster on SDS-PAGE in the presence of Ca(2+), in a manner identical to caleosin extracted from sesame oil bodies. Similar to other known monocot caleosins, the rice caleosin possesses an N-terminal appendix that is absent in dicotyledonous caleosins.

Authors+Show Affiliations

Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22892331

Citation

Chen, Da-Huang, et al. "The Same Oleosin Isoforms Are Present in Oil Bodies of Rice Embryo and Aleurone Layer While Caleosin Exists Only in Those of the Embryo." Plant Physiology and Biochemistry : PPB, vol. 60, 2012, pp. 18-24.
Chen DH, Chyan CL, Jiang PL, et al. The same oleosin isoforms are present in oil bodies of rice embryo and aleurone layer while caleosin exists only in those of the embryo. Plant Physiol Biochem. 2012;60:18-24.
Chen, D. H., Chyan, C. L., Jiang, P. L., Chen, C. S., & Tzen, J. T. (2012). The same oleosin isoforms are present in oil bodies of rice embryo and aleurone layer while caleosin exists only in those of the embryo. Plant Physiology and Biochemistry : PPB, 60, 18-24. https://doi.org/10.1016/j.plaphy.2012.07.022
Chen DH, et al. The Same Oleosin Isoforms Are Present in Oil Bodies of Rice Embryo and Aleurone Layer While Caleosin Exists Only in Those of the Embryo. Plant Physiol Biochem. 2012;60:18-24. PubMed PMID: 22892331.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The same oleosin isoforms are present in oil bodies of rice embryo and aleurone layer while caleosin exists only in those of the embryo. AU - Chen,Da-Huang, AU - Chyan,Chia-Lin, AU - Jiang,Pei-Luen, AU - Chen,Chii-Shiarng, AU - Tzen,Jason T C, Y1 - 2012/08/03/ PY - 2012/05/30/received PY - 2012/07/23/accepted PY - 2012/8/16/entrez PY - 2012/8/16/pubmed PY - 2013/12/25/medline SP - 18 EP - 24 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 60 N2 - Oil bodies of similar sizes were observed in the cells of embryo and aleurone layer of rice seeds, and remained their structural integrity in vitro after isolation. Comparably, two abundant oleosin isoforms were found in both preparations of oil bodies isolated from the embryo and the aleurone layer. Immunological detection and mass spectrometric analyses indicated that the two oleosin isoforms, termed oleosin-H and oleosin-L, in the embryo and those in the aleurone layer were identical proteins encoded by the same genes (BAF12898.1 and BAF15387.1 for oleosin-H and oleosin-L, respectively). In contrast, one caleosin was found in oil bodies isolated from the embryo but not those isolated from the aleurone layer. Immunological staining of rice seeds confirms that oleosin is present in both embryo and aleurone layer while caleosin exists only in embryo. Caleosin extracted from oil bodies of rice embryo migrated faster on SDS-PAGE in the presence of Ca(2+), in a manner identical to caleosin extracted from sesame oil bodies. Similar to other known monocot caleosins, the rice caleosin possesses an N-terminal appendix that is absent in dicotyledonous caleosins. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/22892331/The_same_oleosin_isoforms_are_present_in_oil_bodies_of_rice_embryo_and_aleurone_layer_while_caleosin_exists_only_in_those_of_the_embryo_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(12)00201-X DB - PRIME DP - Unbound Medicine ER -