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Highly sensitive high-throughput profiling of six phytohormones using MS-probe modification and liquid chromatography-tandem mass spectrometry.
Methods Mol Biol. 2012; 918:151-64.MM

Abstract

We describe a method for highly sensitive high-throughput analysis of six major phytohormones; cytokinins (23 species), auxins (7 species), abscisic acid (ABA), gibberellins (GAs, 11 species), salicylic acid (SA), and jasmonic acid (JA). The method consists of solid-phase extraction using 96-well column plates and liquid chromatography coupled with a tandem quadrupole mass spectrometer. In order to improve the quantification limit of negatively charged compounds and especially GAs, we use a chemical modification with an "MS-probe" that contains a quaternary amine moiety. The method requires plant tissue of <100 mg fresh weight for quantification and enables us to analyze large number of plant samples at a time.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kojima M
RIKEN Plant Science Center, Tsurumi, Yokohama, Japan.
Sakakibara H
No affiliation info available

MeSH

AminesChromatography, Ion ExchangeChromatography, LiquidHydrophobic and Hydrophilic InteractionsPhosphorylationPlant Growth RegulatorsPlantsSolid Phase ExtractionTandem Mass Spectrometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22893291

Citation

Kojima, Mikiko, and Hitoshi Sakakibara. "Highly Sensitive High-throughput Profiling of Six Phytohormones Using MS-probe Modification and Liquid Chromatography-tandem Mass Spectrometry." Methods in Molecular Biology (Clifton, N.J.), vol. 918, 2012, pp. 151-64.
Kojima M, Sakakibara H. Highly sensitive high-throughput profiling of six phytohormones using MS-probe modification and liquid chromatography-tandem mass spectrometry. Methods Mol Biol. 2012;918:151-64.
Kojima, M., & Sakakibara, H. (2012). Highly sensitive high-throughput profiling of six phytohormones using MS-probe modification and liquid chromatography-tandem mass spectrometry. Methods in Molecular Biology (Clifton, N.J.), 918, 151-64. https://doi.org/10.1007/978-1-61779-995-2_11
Kojima M, Sakakibara H. Highly Sensitive High-throughput Profiling of Six Phytohormones Using MS-probe Modification and Liquid Chromatography-tandem Mass Spectrometry. Methods Mol Biol. 2012;918:151-64. PubMed PMID: 22893291.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly sensitive high-throughput profiling of six phytohormones using MS-probe modification and liquid chromatography-tandem mass spectrometry. AU - Kojima,Mikiko, AU - Sakakibara,Hitoshi, PY - 2012/8/16/entrez PY - 2012/8/16/pubmed PY - 2012/12/21/medline SP - 151 EP - 64 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 918 N2 - We describe a method for highly sensitive high-throughput analysis of six major phytohormones; cytokinins (23 species), auxins (7 species), abscisic acid (ABA), gibberellins (GAs, 11 species), salicylic acid (SA), and jasmonic acid (JA). The method consists of solid-phase extraction using 96-well column plates and liquid chromatography coupled with a tandem quadrupole mass spectrometer. In order to improve the quantification limit of negatively charged compounds and especially GAs, we use a chemical modification with an "MS-probe" that contains a quaternary amine moiety. The method requires plant tissue of <100 mg fresh weight for quantification and enables us to analyze large number of plant samples at a time. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/22893291/Highly_sensitive_high_throughput_profiling_of_six_phytohormones_using_MS_probe_modification_and_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://dx.doi.org/10.1007/978-1-61779-995-2_11 DB - PRIME DP - Unbound Medicine ER -
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