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Regulation of postnatal forebrain amoeboid microglial cell proliferation and development by the transcription factor Runx1.
J Neurosci 2012; 32(33):11285-98JN

Abstract

Microglia are the immune cells of the nervous system, where they act as resident macrophages during inflammatory events underlying many neuropathological conditions. Microglia derive from primitive myeloid precursors that colonize the nervous system during embryonic development. In the postnatal brain, microglia are initially mitotic, rounded in shape (amoeboid), and phagocytically active. As brain development proceeds, they gradually undergo a transition to a surveillant nonphagocytic state characterized by a highly branched (ramified) morphology. This ramification process is almost recapitulated in reverse during the process of microglia activation in the adult brain, when surveillant microglia undergo a ramified-to-amoeboid morphological transformation and become phagocytic in response to injury or disease. Little is known about the mechanisms controlling amoeboid microglial cell proliferation, activation, and ramification during brain development, despite the critical role of these processes in the establishment of the adult microglia pool and their relevance to microglia activation in the adult brain. Here we show that the mouse transcription factor Runx1, a key regulator of myeloid cell proliferation and differentiation, is expressed in forebrain amoeboid microglia during the first two postnatal weeks. Runx1 expression is then downregulated in ramified microglia. Runx1 inhibits mouse amoeboid microglia proliferation and promotes progression to the ramified state. We show further that Runx1 expression is upregulated in microglia following nerve injury in the adult mouse nervous system. These findings provide insight into the regulation of postnatal microglia activation and maturation to the ramified state and have implications for microglia biology in the developing and injured brain.

Authors+Show Affiliations

Montreal Neurological Institute, McGill University, Montreal, Quebec, H3A 2B4 Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22895712

Citation

Zusso, Morena, et al. "Regulation of Postnatal Forebrain Amoeboid Microglial Cell Proliferation and Development By the Transcription Factor Runx1." The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, vol. 32, no. 33, 2012, pp. 11285-98.
Zusso M, Methot L, Lo R, et al. Regulation of postnatal forebrain amoeboid microglial cell proliferation and development by the transcription factor Runx1. J Neurosci. 2012;32(33):11285-98.
Zusso, M., Methot, L., Lo, R., Greenhalgh, A. D., David, S., & Stifani, S. (2012). Regulation of postnatal forebrain amoeboid microglial cell proliferation and development by the transcription factor Runx1. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 32(33), pp. 11285-98. doi:10.1523/JNEUROSCI.6182-11.2012.
Zusso M, et al. Regulation of Postnatal Forebrain Amoeboid Microglial Cell Proliferation and Development By the Transcription Factor Runx1. J Neurosci. 2012 Aug 15;32(33):11285-98. PubMed PMID: 22895712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of postnatal forebrain amoeboid microglial cell proliferation and development by the transcription factor Runx1. AU - Zusso,Morena, AU - Methot,Laurent, AU - Lo,Rita, AU - Greenhalgh,Andrew D, AU - David,Samuel, AU - Stifani,Stefano, PY - 2012/8/17/entrez PY - 2012/8/17/pubmed PY - 2012/11/3/medline SP - 11285 EP - 98 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J. Neurosci. VL - 32 IS - 33 N2 - Microglia are the immune cells of the nervous system, where they act as resident macrophages during inflammatory events underlying many neuropathological conditions. Microglia derive from primitive myeloid precursors that colonize the nervous system during embryonic development. In the postnatal brain, microglia are initially mitotic, rounded in shape (amoeboid), and phagocytically active. As brain development proceeds, they gradually undergo a transition to a surveillant nonphagocytic state characterized by a highly branched (ramified) morphology. This ramification process is almost recapitulated in reverse during the process of microglia activation in the adult brain, when surveillant microglia undergo a ramified-to-amoeboid morphological transformation and become phagocytic in response to injury or disease. Little is known about the mechanisms controlling amoeboid microglial cell proliferation, activation, and ramification during brain development, despite the critical role of these processes in the establishment of the adult microglia pool and their relevance to microglia activation in the adult brain. Here we show that the mouse transcription factor Runx1, a key regulator of myeloid cell proliferation and differentiation, is expressed in forebrain amoeboid microglia during the first two postnatal weeks. Runx1 expression is then downregulated in ramified microglia. Runx1 inhibits mouse amoeboid microglia proliferation and promotes progression to the ramified state. We show further that Runx1 expression is upregulated in microglia following nerve injury in the adult mouse nervous system. These findings provide insight into the regulation of postnatal microglia activation and maturation to the ramified state and have implications for microglia biology in the developing and injured brain. SN - 1529-2401 UR - https://www.unboundmedicine.com/medline/citation/22895712/Regulation_of_postnatal_forebrain_amoeboid_microglial_cell_proliferation_and_development_by_the_transcription_factor_Runx1_ L2 - http://www.jneurosci.org/cgi/pmidlookup?view=long&pmid=22895712 DB - PRIME DP - Unbound Medicine ER -