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Quantification of abscisic Acid, cytokinin, and auxin content in salt-stressed plant tissues.
Methods Mol Biol. 2012; 913:251-61.MM

Abstract

Plant hormones cytokinins, auxin (indole-3-acetic acid), and abscisic acid are central to regulation of plant growth and defence to abiotic stresses such as salinity. Quantification of the hormone levels and determination of their ratios can reveal different plant strategies to cope with the stress, e.g., suppression of growth or mobilization of plant metabolism. This chapter describes a procedure enabling such quantification. Due to the high variability of these hormones in plant tissues, it is advantageous to determine their content in the same sample. Reverse phase and ion exchange chromatography allows separation of the individual hormone fractions. Hormones as well as their metabolites can be identified and quantified by LC/MS.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Dobrev PI
Institute of Experimental Botany AS CR, Prague, Czech Republic. dobrev@ueb.cas.cz
Vankova R
No affiliation info available

MeSH

Abscisic AcidChromatography, High Pressure LiquidCytokininsIndoleacetic AcidsMass SpectrometryPlant ExtractsPlant LeavesPlant RootsPlantsSalinitySalt ToleranceStress, Physiological

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22895765

Citation

Dobrev, Petre I., and Radomira Vankova. "Quantification of Abscisic Acid, Cytokinin, and Auxin Content in Salt-stressed Plant Tissues." Methods in Molecular Biology (Clifton, N.J.), vol. 913, 2012, pp. 251-61.
Dobrev PI, Vankova R. Quantification of abscisic Acid, cytokinin, and auxin content in salt-stressed plant tissues. Methods Mol Biol. 2012;913:251-61.
Dobrev, P. I., & Vankova, R. (2012). Quantification of abscisic Acid, cytokinin, and auxin content in salt-stressed plant tissues. Methods in Molecular Biology (Clifton, N.J.), 913, 251-61. https://doi.org/10.1007/978-1-61779-986-0_17
Dobrev PI, Vankova R. Quantification of Abscisic Acid, Cytokinin, and Auxin Content in Salt-stressed Plant Tissues. Methods Mol Biol. 2012;913:251-61. PubMed PMID: 22895765.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of abscisic Acid, cytokinin, and auxin content in salt-stressed plant tissues. AU - Dobrev,Petre I, AU - Vankova,Radomira, PY - 2012/8/17/entrez PY - 2012/8/17/pubmed PY - 2013/4/5/medline SP - 251 EP - 61 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 913 N2 - Plant hormones cytokinins, auxin (indole-3-acetic acid), and abscisic acid are central to regulation of plant growth and defence to abiotic stresses such as salinity. Quantification of the hormone levels and determination of their ratios can reveal different plant strategies to cope with the stress, e.g., suppression of growth or mobilization of plant metabolism. This chapter describes a procedure enabling such quantification. Due to the high variability of these hormones in plant tissues, it is advantageous to determine their content in the same sample. Reverse phase and ion exchange chromatography allows separation of the individual hormone fractions. Hormones as well as their metabolites can be identified and quantified by LC/MS. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/22895765/Quantification_of_abscisic_Acid_cytokinin_and_auxin_content_in_salt_stressed_plant_tissues_ L2 - https://dx.doi.org/10.1007/978-1-61779-986-0_17 DB - PRIME DP - Unbound Medicine ER -