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Identification and quantification of cyclolinopeptides in five flaxseed cultivars.
J Agric Food Chem. 2012 Sep 05; 60(35):8571-9.JA

Abstract

Cyclolinopeptides are a group of naturally occurring hydrophobic cyclic peptides found in flaxseed and flax oil that have immunosuppressive activity. This study describes the measurement of flaxseed cyclolinopeptide concentrations using an internal standard HPLC method. In addition, the concentration of cyclolinopeptides in the seed of Canadian flax cultivars grown at two locations over two years is reported. The data are consistent with the formation of flaxseed cyclolinopeptides from two ribosome-derived precursors. Each precursor protein includes the sequences corresponding to three cyclolinopeptides from which those cyclolinopeptides are presumably derived by precursor processing. The concentrations of cyclolinopeptides C and E, which are encoded by the same gene sequence, are highly correlated, and the concentrations of cyclolinopeptides D, F, and G, which are encoded by a second gene sequence, are also highly correlated. The strong correlation between the cyclolinopeptides arising from the same gene may prove to be important in understanding how peptide concentration is controlled. Additional research may lead to approaches to improve flax either as a platform for peptide production or as a source of oil with improved drying properties and flavor.

Authors+Show Affiliations

Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22897677

Citation

Gui, Bo, et al. "Identification and Quantification of Cyclolinopeptides in Five Flaxseed Cultivars." Journal of Agricultural and Food Chemistry, vol. 60, no. 35, 2012, pp. 8571-9.
Gui B, Shim YY, Datla RS, et al. Identification and quantification of cyclolinopeptides in five flaxseed cultivars. J Agric Food Chem. 2012;60(35):8571-9.
Gui, B., Shim, Y. Y., Datla, R. S., Covello, P. S., Stone, S. L., & Reaney, M. J. (2012). Identification and quantification of cyclolinopeptides in five flaxseed cultivars. Journal of Agricultural and Food Chemistry, 60(35), 8571-9. https://doi.org/10.1021/jf301847u
Gui B, et al. Identification and Quantification of Cyclolinopeptides in Five Flaxseed Cultivars. J Agric Food Chem. 2012 Sep 5;60(35):8571-9. PubMed PMID: 22897677.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and quantification of cyclolinopeptides in five flaxseed cultivars. AU - Gui,Bo, AU - Shim,Youn Young, AU - Datla,Raju S S, AU - Covello,Patrick S, AU - Stone,Sandra L, AU - Reaney,Martin J T, Y1 - 2012/08/24/ PY - 2012/8/18/entrez PY - 2012/8/18/pubmed PY - 2013/1/8/medline SP - 8571 EP - 9 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 60 IS - 35 N2 - Cyclolinopeptides are a group of naturally occurring hydrophobic cyclic peptides found in flaxseed and flax oil that have immunosuppressive activity. This study describes the measurement of flaxseed cyclolinopeptide concentrations using an internal standard HPLC method. In addition, the concentration of cyclolinopeptides in the seed of Canadian flax cultivars grown at two locations over two years is reported. The data are consistent with the formation of flaxseed cyclolinopeptides from two ribosome-derived precursors. Each precursor protein includes the sequences corresponding to three cyclolinopeptides from which those cyclolinopeptides are presumably derived by precursor processing. The concentrations of cyclolinopeptides C and E, which are encoded by the same gene sequence, are highly correlated, and the concentrations of cyclolinopeptides D, F, and G, which are encoded by a second gene sequence, are also highly correlated. The strong correlation between the cyclolinopeptides arising from the same gene may prove to be important in understanding how peptide concentration is controlled. Additional research may lead to approaches to improve flax either as a platform for peptide production or as a source of oil with improved drying properties and flavor. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/22897677/Identification_and_quantification_of_cyclolinopeptides_in_five_flaxseed_cultivars_ L2 - https://dx.doi.org/10.1021/jf301847u DB - PRIME DP - Unbound Medicine ER -