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Distribution of cyclolinopeptides in flaxseed fractions and products.
J Agric Food Chem. 2012 Sep 05; 60(35):8580-9.JA

Abstract

Hydrophobic cyclic peptides, termed cyclolinopeptides, found in flaxseed are known for their immunosuppressive activity. This study is the first report of the occurrence of cyclolinopeptides in flaxseed fractions and products produced by aqueous processing and cold pressing. The distribution of cyclolinopeptides in flaxseed was determined after processing of flaxseed by various industrial and laboratory processes. Extracts of the water-soluble mucilage did not contain cyclolinopeptides. The cotyledon had the highest concentration of cyclolinopeptides, whereas seed coat had lower levels. An oil body fraction separated from seed after homogenization in water, followed by centrifugation, had the highest concentration of cyclolinopeptides of the fractions produced by this method. Further washing of the oil body fraction led to a loss of cyclolinopeptides. When oilseed was extruded using an expeller press, cyclolinopeptides were found in greater concentrations in crude oil and the solid sediment present in the oil fraction than in meal or the unprocessed seed. The concentration of cyclolinopeptides in crude flaxseed oil immediately after pressing was much higher than that observed in flaxseed oils purchased from a retail outlet. The effect of oil refining treatments on the removal of cyclolinopeptides was also tested. Acid degumming using aqueous H(3)PO(4) removed cyclolinopeptides from crude flaxseed oil. Alkali refining was less effective as this treatment failed to remove all peptides equally. This work illustrates ways that cyclolinopeptides may be extracted from flaxseed oil that could be developed for large-scale industrial extraction. The ability to extract cyclolinopeptides on a larger scale would allow faster exploitation of commercial applications of these molecules and provide the flaxseed industry with value-added coproducts.

Authors+Show Affiliations

Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22897751

Citation

Gui, Bo, et al. "Distribution of Cyclolinopeptides in Flaxseed Fractions and Products." Journal of Agricultural and Food Chemistry, vol. 60, no. 35, 2012, pp. 8580-9.
Gui B, Shim YY, Reaney MJ. Distribution of cyclolinopeptides in flaxseed fractions and products. J Agric Food Chem. 2012;60(35):8580-9.
Gui, B., Shim, Y. Y., & Reaney, M. J. (2012). Distribution of cyclolinopeptides in flaxseed fractions and products. Journal of Agricultural and Food Chemistry, 60(35), 8580-9. https://doi.org/10.1021/jf3023832
Gui B, Shim YY, Reaney MJ. Distribution of Cyclolinopeptides in Flaxseed Fractions and Products. J Agric Food Chem. 2012 Sep 5;60(35):8580-9. PubMed PMID: 22897751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of cyclolinopeptides in flaxseed fractions and products. AU - Gui,Bo, AU - Shim,Youn Young, AU - Reaney,Martin J T, Y1 - 2012/08/24/ PY - 2012/8/18/entrez PY - 2012/8/18/pubmed PY - 2013/1/8/medline SP - 8580 EP - 9 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 60 IS - 35 N2 - Hydrophobic cyclic peptides, termed cyclolinopeptides, found in flaxseed are known for their immunosuppressive activity. This study is the first report of the occurrence of cyclolinopeptides in flaxseed fractions and products produced by aqueous processing and cold pressing. The distribution of cyclolinopeptides in flaxseed was determined after processing of flaxseed by various industrial and laboratory processes. Extracts of the water-soluble mucilage did not contain cyclolinopeptides. The cotyledon had the highest concentration of cyclolinopeptides, whereas seed coat had lower levels. An oil body fraction separated from seed after homogenization in water, followed by centrifugation, had the highest concentration of cyclolinopeptides of the fractions produced by this method. Further washing of the oil body fraction led to a loss of cyclolinopeptides. When oilseed was extruded using an expeller press, cyclolinopeptides were found in greater concentrations in crude oil and the solid sediment present in the oil fraction than in meal or the unprocessed seed. The concentration of cyclolinopeptides in crude flaxseed oil immediately after pressing was much higher than that observed in flaxseed oils purchased from a retail outlet. The effect of oil refining treatments on the removal of cyclolinopeptides was also tested. Acid degumming using aqueous H(3)PO(4) removed cyclolinopeptides from crude flaxseed oil. Alkali refining was less effective as this treatment failed to remove all peptides equally. This work illustrates ways that cyclolinopeptides may be extracted from flaxseed oil that could be developed for large-scale industrial extraction. The ability to extract cyclolinopeptides on a larger scale would allow faster exploitation of commercial applications of these molecules and provide the flaxseed industry with value-added coproducts. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/22897751/Distribution_of_cyclolinopeptides_in_flaxseed_fractions_and_products_ L2 - https://dx.doi.org/10.1021/jf3023832 DB - PRIME DP - Unbound Medicine ER -