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MANF regulates dopaminergic neuron development in larval zebrafish.
Dev Biol 2012; 370(2):237-49DB

Abstract

Mesencephalic astrocyte derived neurotrophic factor (MANF) is recognized as a dopaminergic neurotrophic factor, which can protect dopaminergic neurons from neurotoxic damage. However, little is known about the function of MANF during the vertebrate development. Here, we report that MANF expression is widespread during embryonic development and in adult organs analyzed by qPCR and in situ hybridization in zebrafish. Knockdown of MANF expression with antisense splice-blocking morpholino oligonucleotides resulted in no apparent abnormal phenotype. Nevertheless, the dopamine level of MANF morphants was lower than that of the wild type larvae, the expression levels of the two tyrosine hydroxylase gene transcripts were decreased and a decrease in neuron number in certain groups of th1 and th2 cells in the diencephalon region in MANF morphants was observed. These defects were rescued by injection of exogenous manf mRNA. Strikingly, manf mRNA could partly restore the decrease of th1 positive cells in Nr4a2-deficient larvae. These results suggest that MANF is involved in the regulation of the development of dopaminergic system in zebrafish.

Authors+Show Affiliations

Neuroscience Center and Institute of Biomedicine/AnatomyUniversity of Helsinki, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22898306

Citation

Chen, Y-C, et al. "MANF Regulates Dopaminergic Neuron Development in Larval Zebrafish." Developmental Biology, vol. 370, no. 2, 2012, pp. 237-49.
Chen YC, Sundvik M, Rozov S, et al. MANF regulates dopaminergic neuron development in larval zebrafish. Dev Biol. 2012;370(2):237-49.
Chen, Y. C., Sundvik, M., Rozov, S., Priyadarshini, M., & Panula, P. (2012). MANF regulates dopaminergic neuron development in larval zebrafish. Developmental Biology, 370(2), pp. 237-49. doi:10.1016/j.ydbio.2012.07.030.
Chen YC, et al. MANF Regulates Dopaminergic Neuron Development in Larval Zebrafish. Dev Biol. 2012 Oct 15;370(2):237-49. PubMed PMID: 22898306.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MANF regulates dopaminergic neuron development in larval zebrafish. AU - Chen,Y-C, AU - Sundvik,M, AU - Rozov,S, AU - Priyadarshini,M, AU - Panula,P, Y1 - 2012/08/07/ PY - 2012/01/26/received PY - 2012/06/08/revised PY - 2012/07/31/accepted PY - 2012/8/18/entrez PY - 2012/8/18/pubmed PY - 2012/12/10/medline SP - 237 EP - 49 JF - Developmental biology JO - Dev. Biol. VL - 370 IS - 2 N2 - Mesencephalic astrocyte derived neurotrophic factor (MANF) is recognized as a dopaminergic neurotrophic factor, which can protect dopaminergic neurons from neurotoxic damage. However, little is known about the function of MANF during the vertebrate development. Here, we report that MANF expression is widespread during embryonic development and in adult organs analyzed by qPCR and in situ hybridization in zebrafish. Knockdown of MANF expression with antisense splice-blocking morpholino oligonucleotides resulted in no apparent abnormal phenotype. Nevertheless, the dopamine level of MANF morphants was lower than that of the wild type larvae, the expression levels of the two tyrosine hydroxylase gene transcripts were decreased and a decrease in neuron number in certain groups of th1 and th2 cells in the diencephalon region in MANF morphants was observed. These defects were rescued by injection of exogenous manf mRNA. Strikingly, manf mRNA could partly restore the decrease of th1 positive cells in Nr4a2-deficient larvae. These results suggest that MANF is involved in the regulation of the development of dopaminergic system in zebrafish. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/22898306/MANF_regulates_dopaminergic_neuron_development_in_larval_zebrafish_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(12)00414-9 DB - PRIME DP - Unbound Medicine ER -