Tags

Type your tag names separated by a space and hit enter

Ki-67 grading of nonfunctioning pancreatic neuroendocrine tumors on histologic samples obtained by EUS-guided fine-needle tissue acquisition: a prospective study.
Gastrointest Endosc. 2012 Sep; 76(3):570-7.GE

Abstract

BACKGROUND

Preoperative determination of Ki-67 expression, an important prognostic factor for grading nonfunctioning pancreatic endocrine tumors (NF-PETs), remains an important clinical challenge.

OBJECTIVE

To prospectively evaluate the feasibility, yield, and clinical impact of EUS-guided fine-needle tissue acquisition (EUS-FNTA) with a large-gauge needle to obtain tissue samples for histologic diagnosis and Ki-67 analysis in patients with suspected NF-PETs.

DESIGN

Prospective cohort study.

SETTING

Tertiary-care academic medical center.

PATIENTS

Consecutive patients with a single pancreatic lesion suspicious for NF-PET on imaging.

INTERVENTION

EUS-FNTA with a 19-gauge needle.

MAIN OUTCOME MEASUREMENTS

Feasibility and yield of EUS-FNTA for diagnosis and Ki-67 expression determination.

RESULTS

Thirty patients (mean [± SD] age 55.7 ± 14.9 years), with a mean (± SD) lesion size of 16.9 ± 6.1 mm were enrolled. EUS-FNTA was successfully performed without complications in all patients, with a mean (± SD) of 2.7 ± 0.5 passes per patient. Adequate samples for histologic examination were obtained in 28 of the 30 patients (93.3%). Ki-67 determination could be performed in 26 of these 28 patients (92.9%, 86.6% overall), 12 of whom underwent surgical resection. Preoperative and postoperative Ki-67 proliferation indexes were concordant in 10 patients (83.3%), whereas 2 patients were upstaged from G1 to G2 or downstaged from G2 to G1, respectively.

LIMITATIONS

Single center study with a single operator.

CONCLUSION

In patients with suspected nonfunctioning low-grade to intermediate-grade pancreatic neuroendocrine tumors (p-NETs), retrieval of tissue specimens with EUS-FNTA by using a 19-gauge needle is safe, feasible, and highly accurate for both diagnosis and Ki-67 determination. A Ki-67 proliferative index acquired through this technique might be of great help for further therapeutic decisions.

Authors+Show Affiliations

Digestive Endoscopy Unit, Divisionof Digestive and Liver Disease, Catholic University, Rome, Italy. albertolarghi@yahoo.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article

Language

eng

PubMed ID

22898415

Citation

Larghi, Alberto, et al. "Ki-67 Grading of Nonfunctioning Pancreatic Neuroendocrine Tumors On Histologic Samples Obtained By EUS-guided Fine-needle Tissue Acquisition: a Prospective Study." Gastrointestinal Endoscopy, vol. 76, no. 3, 2012, pp. 570-7.
Larghi A, Capurso G, Carnuccio A, et al. Ki-67 grading of nonfunctioning pancreatic neuroendocrine tumors on histologic samples obtained by EUS-guided fine-needle tissue acquisition: a prospective study. Gastrointest Endosc. 2012;76(3):570-7.
Larghi, A., Capurso, G., Carnuccio, A., Ricci, R., Alfieri, S., Galasso, D., Lugli, F., Bianchi, A., Panzuto, F., De Marinis, L., Falconi, M., Delle Fave, G., Doglietto, G. B., Costamagna, G., & Rindi, G. (2012). Ki-67 grading of nonfunctioning pancreatic neuroendocrine tumors on histologic samples obtained by EUS-guided fine-needle tissue acquisition: a prospective study. Gastrointestinal Endoscopy, 76(3), 570-7. https://doi.org/10.1016/j.gie.2012.04.477
Larghi A, et al. Ki-67 Grading of Nonfunctioning Pancreatic Neuroendocrine Tumors On Histologic Samples Obtained By EUS-guided Fine-needle Tissue Acquisition: a Prospective Study. Gastrointest Endosc. 2012;76(3):570-7. PubMed PMID: 22898415.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ki-67 grading of nonfunctioning pancreatic neuroendocrine tumors on histologic samples obtained by EUS-guided fine-needle tissue acquisition: a prospective study. AU - Larghi,Alberto, AU - Capurso,Gabriele, AU - Carnuccio,Antonella, AU - Ricci,Riccardo, AU - Alfieri,Sergio, AU - Galasso,Domenico, AU - Lugli,Francesca, AU - Bianchi,Antonio, AU - Panzuto,Francesco, AU - De Marinis,Laura, AU - Falconi,Massimo, AU - Delle Fave,Gianfranco, AU - Doglietto,Giovanni Battista, AU - Costamagna,Guido, AU - Rindi,Guido, PY - 2012/02/04/received PY - 2012/04/25/accepted PY - 2012/8/18/entrez PY - 2012/8/18/pubmed PY - 2013/1/24/medline SP - 570 EP - 7 JF - Gastrointestinal endoscopy JO - Gastrointest Endosc VL - 76 IS - 3 N2 - BACKGROUND: Preoperative determination of Ki-67 expression, an important prognostic factor for grading nonfunctioning pancreatic endocrine tumors (NF-PETs), remains an important clinical challenge. OBJECTIVE: To prospectively evaluate the feasibility, yield, and clinical impact of EUS-guided fine-needle tissue acquisition (EUS-FNTA) with a large-gauge needle to obtain tissue samples for histologic diagnosis and Ki-67 analysis in patients with suspected NF-PETs. DESIGN: Prospective cohort study. SETTING: Tertiary-care academic medical center. PATIENTS: Consecutive patients with a single pancreatic lesion suspicious for NF-PET on imaging. INTERVENTION: EUS-FNTA with a 19-gauge needle. MAIN OUTCOME MEASUREMENTS: Feasibility and yield of EUS-FNTA for diagnosis and Ki-67 expression determination. RESULTS: Thirty patients (mean [± SD] age 55.7 ± 14.9 years), with a mean (± SD) lesion size of 16.9 ± 6.1 mm were enrolled. EUS-FNTA was successfully performed without complications in all patients, with a mean (± SD) of 2.7 ± 0.5 passes per patient. Adequate samples for histologic examination were obtained in 28 of the 30 patients (93.3%). Ki-67 determination could be performed in 26 of these 28 patients (92.9%, 86.6% overall), 12 of whom underwent surgical resection. Preoperative and postoperative Ki-67 proliferation indexes were concordant in 10 patients (83.3%), whereas 2 patients were upstaged from G1 to G2 or downstaged from G2 to G1, respectively. LIMITATIONS: Single center study with a single operator. CONCLUSION: In patients with suspected nonfunctioning low-grade to intermediate-grade pancreatic neuroendocrine tumors (p-NETs), retrieval of tissue specimens with EUS-FNTA by using a 19-gauge needle is safe, feasible, and highly accurate for both diagnosis and Ki-67 determination. A Ki-67 proliferative index acquired through this technique might be of great help for further therapeutic decisions. SN - 1097-6779 UR - https://www.unboundmedicine.com/medline/citation/22898415/Ki_67_grading_of_nonfunctioning_pancreatic_neuroendocrine_tumors_on_histologic_samples_obtained_by_EUS_guided_fine_needle_tissue_acquisition:_a_prospective_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-5107(12)02321-8 DB - PRIME DP - Unbound Medicine ER -