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Growth-inhibitory effects of pigmented rice bran extracts and three red bran fractions against human cancer cells: relationships with composition and antioxidative activities.
J Agric Food Chem. 2012 Sep 12; 60(36):9151-61.JA

Abstract

We determined the phenolic, anthocyanin, and proanthocyanidin content of three brown, purple, and red rice brans isolated from different rice varieties using HPLC-PDA with the aid of 27 standards of known structure and matching unknown peaks to a spectral library of known compounds. Antioxidative capacities were determined by DPPH and ORAC and cell-inhibiting effects using an MTT assay. Based on the calculated IC(50) values, the light-brown bran had no effect, the purple bran exhibited a minor effect on leukemia and cervical cancer cells, and the red bran exhibited strong inhibitory effects on leukemia, cervical, and stomach cancer cells. High concentrations of protocatechuic acid and anthocyanins in purple bran and proanthocyanidins in red bran were identified. The red bran was further fractionated on a Sephadex column. Fraction 3 rich in proanthocyanidin oligomers and polymers had the greatest activity. Red bran has the potential to serve as a functional food supplement for human consumption.

Authors+Show Affiliations

Dale Bumpers National Rice Research Center, Agricultural Research Service, U.S. Department of Agriculture, 2890 Highway 130 East, Stuttgart, Arkansas 72160, USA. ming.chen@ars.usda.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22900641

Citation

Chen, Ming-Hsuan, et al. "Growth-inhibitory Effects of Pigmented Rice Bran Extracts and Three Red Bran Fractions Against Human Cancer Cells: Relationships With Composition and Antioxidative Activities." Journal of Agricultural and Food Chemistry, vol. 60, no. 36, 2012, pp. 9151-61.
Chen MH, Choi SH, Kozukue N, et al. Growth-inhibitory effects of pigmented rice bran extracts and three red bran fractions against human cancer cells: relationships with composition and antioxidative activities. J Agric Food Chem. 2012;60(36):9151-61.
Chen, M. H., Choi, S. H., Kozukue, N., Kim, H. J., & Friedman, M. (2012). Growth-inhibitory effects of pigmented rice bran extracts and three red bran fractions against human cancer cells: relationships with composition and antioxidative activities. Journal of Agricultural and Food Chemistry, 60(36), 9151-61. https://doi.org/10.1021/jf3025453
Chen MH, et al. Growth-inhibitory Effects of Pigmented Rice Bran Extracts and Three Red Bran Fractions Against Human Cancer Cells: Relationships With Composition and Antioxidative Activities. J Agric Food Chem. 2012 Sep 12;60(36):9151-61. PubMed PMID: 22900641.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth-inhibitory effects of pigmented rice bran extracts and three red bran fractions against human cancer cells: relationships with composition and antioxidative activities. AU - Chen,Ming-Hsuan, AU - Choi,Suk Hyun, AU - Kozukue,Nobuyuke, AU - Kim,Hyun-Jeong, AU - Friedman,Mendel, Y1 - 2012/08/27/ PY - 2012/8/21/entrez PY - 2012/8/21/pubmed PY - 2013/1/25/medline SP - 9151 EP - 61 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 60 IS - 36 N2 - We determined the phenolic, anthocyanin, and proanthocyanidin content of three brown, purple, and red rice brans isolated from different rice varieties using HPLC-PDA with the aid of 27 standards of known structure and matching unknown peaks to a spectral library of known compounds. Antioxidative capacities were determined by DPPH and ORAC and cell-inhibiting effects using an MTT assay. Based on the calculated IC(50) values, the light-brown bran had no effect, the purple bran exhibited a minor effect on leukemia and cervical cancer cells, and the red bran exhibited strong inhibitory effects on leukemia, cervical, and stomach cancer cells. High concentrations of protocatechuic acid and anthocyanins in purple bran and proanthocyanidins in red bran were identified. The red bran was further fractionated on a Sephadex column. Fraction 3 rich in proanthocyanidin oligomers and polymers had the greatest activity. Red bran has the potential to serve as a functional food supplement for human consumption. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/22900641/Growth_inhibitory_effects_of_pigmented_rice_bran_extracts_and_three_red_bran_fractions_against_human_cancer_cells:_relationships_with_composition_and_antioxidative_activities_ L2 - https://doi.org/10.1021/jf3025453 DB - PRIME DP - Unbound Medicine ER -