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Antioxidant, antihemolytic, and inhibitory activities of endemic Primula heterochroma against Fe2+-induced lipid peroxidation and oxidative stress in rat brain in vitro.
Pharm Biol. 2012 Nov; 50(11):1391-6.PB

Abstract

CONTEXT

The genus Primula (Primulaceae) has been used in traditional medicine to treat convulsion and microbial or viral infections.

OBJECTIVE

In the present study, we evaluate antioxidant, antihemolytic, and protective effects of flavonoid-rich fractions of endemic Primula heterochroma Stapf. (Primulaceae) against Fe(2+)-induced lipid peroxidation and oxidative stress in rat brain in vitro.

MATERIALS AND METHODS

Aerial parts of plant were defatted and extracted with 60% acetone. Then, 60% acetone extract was fractionated sequentially with n-hexane, ethyl acetate, and water. Antioxidant activity of fractions was evaluated by employing six different assays, i.e., 1,1-diphenyl-2-picryl hydrazyl (DPPH) and hydrogen peroxide scavenging, metal chelating and reducing power activities and hemoglobin-induced linoleic acid system and Fe(2+)-induced lipid peroxidation and oxidative stress in rat brain. Also, its antihemolytic activity was determined using 2,2'-azobis(2-amidinopropane) dihydrochloride-induced hemolysis in rat erythrocyts.

RESULTS

Among the flavonoid-rich fractions of Primula heterochroma, aqueous fraction demonstrated the most protective effect through decreasing brain thiobarbituric acid reactive substances (TBARS) levels at a dose 200 μg mL(-1) (40%, p < 0.001 versus iron group). Also, the aqueous fraction showed better activity in Fe(2+) chelating (89 ± 3.8 μg mL(-1)) and DPPH radical scavenging (394.4 ± 18.4 μg mL(-1)) models than other fractions. The probable protective mechanism of flavonoid-rich fractions may be attributed to their Fe(2+) chelating, DPPH radical scavenging and reducing power activities. Also, the n-hexane fraction demonstrated a higher protective effect in the hemoglobin-induced linoleic acid system and 2,2'-azobis(2-amidinopropane) dihydrochloride-induced hemolysis (67 ± 2.6 μg mL(-1)).

DISCUSSION AND CONCLUSION

Results of this study demonstrate Primula heterochroma is a rich source of natural antioxidant compounds.

Authors+Show Affiliations

Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

22900682

Citation

Alinezhad, Heshmatollah, et al. "Antioxidant, Antihemolytic, and Inhibitory Activities of Endemic Primula Heterochroma Against Fe2+-induced Lipid Peroxidation and Oxidative Stress in Rat Brain in Vitro." Pharmaceutical Biology, vol. 50, no. 11, 2012, pp. 1391-6.
Alinezhad H, Zare M, Nabavi SF, et al. Antioxidant, antihemolytic, and inhibitory activities of endemic Primula heterochroma against Fe2+-induced lipid peroxidation and oxidative stress in rat brain in vitro. Pharm Biol. 2012;50(11):1391-6.
Alinezhad, H., Zare, M., Nabavi, S. F., Naqinezhad, A., & Nabavi, S. M. (2012). Antioxidant, antihemolytic, and inhibitory activities of endemic Primula heterochroma against Fe2+-induced lipid peroxidation and oxidative stress in rat brain in vitro. Pharmaceutical Biology, 50(11), 1391-6. https://doi.org/10.3109/13880209.2012.676050
Alinezhad H, et al. Antioxidant, Antihemolytic, and Inhibitory Activities of Endemic Primula Heterochroma Against Fe2+-induced Lipid Peroxidation and Oxidative Stress in Rat Brain in Vitro. Pharm Biol. 2012;50(11):1391-6. PubMed PMID: 22900682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant, antihemolytic, and inhibitory activities of endemic Primula heterochroma against Fe2+-induced lipid peroxidation and oxidative stress in rat brain in vitro. AU - Alinezhad,Heshmatollah, AU - Zare,Mahboobeh, AU - Nabavi,Seyed Fazel, AU - Naqinezhad,Alireza, AU - Nabavi,Seyed Mohammad, Y1 - 2012/09/11/ PY - 2012/8/21/entrez PY - 2012/8/21/pubmed PY - 2013/3/2/medline SP - 1391 EP - 6 JF - Pharmaceutical biology JO - Pharm Biol VL - 50 IS - 11 N2 - CONTEXT: The genus Primula (Primulaceae) has been used in traditional medicine to treat convulsion and microbial or viral infections. OBJECTIVE: In the present study, we evaluate antioxidant, antihemolytic, and protective effects of flavonoid-rich fractions of endemic Primula heterochroma Stapf. (Primulaceae) against Fe(2+)-induced lipid peroxidation and oxidative stress in rat brain in vitro. MATERIALS AND METHODS: Aerial parts of plant were defatted and extracted with 60% acetone. Then, 60% acetone extract was fractionated sequentially with n-hexane, ethyl acetate, and water. Antioxidant activity of fractions was evaluated by employing six different assays, i.e., 1,1-diphenyl-2-picryl hydrazyl (DPPH) and hydrogen peroxide scavenging, metal chelating and reducing power activities and hemoglobin-induced linoleic acid system and Fe(2+)-induced lipid peroxidation and oxidative stress in rat brain. Also, its antihemolytic activity was determined using 2,2'-azobis(2-amidinopropane) dihydrochloride-induced hemolysis in rat erythrocyts. RESULTS: Among the flavonoid-rich fractions of Primula heterochroma, aqueous fraction demonstrated the most protective effect through decreasing brain thiobarbituric acid reactive substances (TBARS) levels at a dose 200 μg mL(-1) (40%, p < 0.001 versus iron group). Also, the aqueous fraction showed better activity in Fe(2+) chelating (89 ± 3.8 μg mL(-1)) and DPPH radical scavenging (394.4 ± 18.4 μg mL(-1)) models than other fractions. The probable protective mechanism of flavonoid-rich fractions may be attributed to their Fe(2+) chelating, DPPH radical scavenging and reducing power activities. Also, the n-hexane fraction demonstrated a higher protective effect in the hemoglobin-induced linoleic acid system and 2,2'-azobis(2-amidinopropane) dihydrochloride-induced hemolysis (67 ± 2.6 μg mL(-1)). DISCUSSION AND CONCLUSION: Results of this study demonstrate Primula heterochroma is a rich source of natural antioxidant compounds. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/22900682/Antioxidant_antihemolytic_and_inhibitory_activities_of_endemic_Primula_heterochroma_against_Fe2+_induced_lipid_peroxidation_and_oxidative_stress_in_rat_brain_in_vitro_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2012.676050 DB - PRIME DP - Unbound Medicine ER -